The article discusses the importance of the role of biological diversity of plants, including the problem of specific disappearing tomato varieties which are currently under threat of extinction due to environmental degradation and progress in science and technology, the importance of using them as a source material in breeding work in the aspect of adaptation to local environmental conditions, as well as the importance of preserving, restoring these materials, and obtaining clean lines from them. In the course of the study, several interline F1 hybrids were obtained from pure lines obtained from local and foreign tomato varieties. The article also reports on the results of studying the combining ability of pure lines used in the selection of parental pairs, giving high productivity and high-quality yield. Combining pure lines with a high combinative ability, we obtained F1 hybrids with the effect of heterosis, adapted to local ground conditions. Our work also presents data on the restoration of seeds of high-yielding, high-quality tomato varieties of the Scientific Research Institute of Vegetable Breeding. which are stored in the Gene bank of the Institute of Genetic Resources of the National Academy of Sciences of Azerbaijan. In the presented article, in addition to the above, the general and distinctive aspects of modern scientific hypotheses about methods and techniques for  creating F1 hybrids with the effect of heterosis are discussed, depending on the genetic origin of the variety of plant and the influence of local agroecological factors.