vetpressАграрная наукаAgrarian science0869-81552686-701XРедакция журнала "Аграрная наука"10.32634/0869-8155-2022-364-11-41-44vetpress-2350Research ArticleЗООТЕХНИЯ И ВЕТЕРИНАРИЯZOOTECHNICS AND VETERINARY MEDICINEОпределение антигенных свойств хиноидного радиотоксина с помощью реакции непрямой гемагглютинации (РНГА)Determination of the antigenic properties of quinoidr adiotoxin using the indirect hemagglutination test (IHA)https://orcid.org/0000-0002-2061-489XКамаловаЭ. Р.KamalovaZ. R.

аспирант, старший лаборант, сектор радиационной  иммунологии, отделение радиобиологии 

Научный городок-2, Казань, 420075, Российская Федерация 

 Postgraduate Student, Senior Laboratory Assistant, Sector of Radiation Immunology, Department of RadiobiologyNauchny gorodok-2, Kazan, 420075, Russian Federation 

zikama01@mail.ruhttps://orcid.org/0000-0002-7411-0005ГайнуллинР. Р.GainullinR. R.

кандидат биологических наук, старший научный сотрудник, сектор радиационной иммунологии,  отделение радиобиологии 

Научный городок-2, Казань, 420075, Российская Федерация 

 Candidate of Biological Sciences, Senior Researcher, Sector of Radiation Immunology, Department of RadiobiologyNauchny gorodok-2, Kazan, 420075, Russian Federation 

gairuslan10@mail.ruhttps://orcid.org/0000-0002-8595-0800НизамовР. Н.NizamovR. N.

доктор ветеринарных наук, главный научный сотрудник, лаборатория радиационной  безопасности и ветеринарно-санитарной  экспертизы, отделение радиобиологии 

Научный городок-2, Казань, 420075, Российская Федерация 

 Doctor of Veterinary Sciences, Chief Researcher, Laboratory of radiation safety and veterinary and sanitary expertise, Department of RadiobiologyNauchny gorodok-2, Kazan, 420075, Russian Federation 

r.n.nizamov@mail.ruhttps://orcid.org/0000-0003-3652-1978КурбангалеевЯ. М.KurbangaleevYa. M.

кандидат биологических наук, ведущий научный  сотрудник, сектор радиационной иммунологии,  отделение радиобиологии 

Научный городок-2, Казань, 420075, Российская Федерация 

Candidate of Biological Sciences, Leading, Researcher, Sector of Radiation Immunology, Department of Radiobiology,Nauchny gorodok-2, Kazan, 420075, Russian Federation 

yag72@yandex.ruhttps://orcid.org/0000-0002-2563-5948ШакуровМ. М.ShakurovM. M.

кандидат биологических наук, ведущий научный сотрудник, сектор радиационной иммунологии,  отделение радиобиологии 

Научный городок-2, Казань, 420075, Российская Федерация 

 Candidate of Biological Sciences, Leading Researcher, Sector of Radiation Immunology, Department of RadiobiologyNauchny gorodok-2, Kazan, 420075, Russian Federation 

MulanurShakurov@mail.comФедеральный центр токсикологической, радиационной и биологической безопасностиРоссияFederal Center for Toxicological, Radiation and Biological SafetyRussian Federation2022110120231114144Copyright © Камалова Э.Р., Гайнуллин Р.Р., Низамов Р.Н., Курбангалеев Я.М., Шакуров М.М., 20232023Камалова Э.Р., Гайнуллин Р.Р., Низамов Р.Н., Курбангалеев Я.М., Шакуров М.М.Kamalova Z.R., Gainullin R.R., Nizamov R.N., Kurbangaleev Y.M., Shakurov M.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vetpress.ru/jour/article/view/2350

Актуальность. В статье приведены результаты проведенных опытов по определению антигенныхсвойств (способности хиноидного радиотоксина синтезировать антирадиотоксические антитела) растительного радиотоксина с помощью РНГА.Методы. Для проведения РНГА-теста использовали антигенный вариант эритроцитарного диагностикума (АГЭД), представляющего собой сенсибилизированные лучевыми антигенами эритроциты барана. В качестве контрольных (положительных) противолучевых антител в реакции использовали противолучевые антитела, полученные путем гипериммунизации кроликов лучевым антигеном — радиотоксином, выделенным из печеночной ткани летально облученных овец; испытуемые антитоксические антитела — гипериммунные сыворотки от гипериммунизированных хиноидным (растительным) радиотоксином овец, отрицательная сыворотка, полученная от интактных (необлученных, непривитых никакими вакцинами) овец, гетерологичные сыворотки (противоожоговая, противоколибактериозную). Реакцию ставили на микропланшетах в объеме 50 мкл и учитывали по четырехбалльной системе в крестах.Результаты. Изучение антигенной активности испытуемого лучевого антигена — растительного хиноидного радиотоксина (ХРТ) — с использованием РНГА-теста показали, что испытуемый антиген обладает высокой антигенной активностью, индуцируя в организме иммунизированных животных (овец) синтез антирадиотоксических антител, титры которых значительно превосходят таковые иммунизированных печеночным (животным) антигеном — радиотоксином животных. Хиноидный радиотоксин, полученный из растительной ткани (клубней картофеля) и конъюгированный с белково-липоидной группой (неполным адъювантом Фрейнда — НАФ), обладает высокой антигенностью, индуцируя синтез специфических антирадиотоксических антител, которые могут быть использованы в качестве важнейшего компонента иммунохимической тест-системы — сенситина, используемого для сенсибилизации микро- и наночастиц бентонита при конструировании противорадиационного антительного варианта бентонитового диагностикума (АТБД).

Relevance. The article presents the results of experiments to determine the antigenic properties (the ability of quinoid radiotoxin to synthesize antiradiotoxic antibodies) of plant radiotoxin using IHA.Methods. An antigenic variant of the erythrocyte diagnosticum (AGED), which is a ram erythrocytes sensitized by radiation antigens, was used to conduct the IHA-test. As control (positive) antiradiation antibodies in the reaction, we used antiradiation antibodies obtained by hyperimmunization of rabbits with a radiation antigen — radiotoxin isolated from the liver tissue of lethally irradiated sheep, tested antitoxic antibodies — hyperimmune sera from sheep hyperimmunized with quinoid (plant) radiotoxin, negative serum obtained from intact (non-irradiated, not vaccinated with any vaccines) sheep, heterologous sera (antiburn, anticolibacillary). The reaction was placed on microplates in a volume of 50 ml and was taken into account according to a four-point system in crosses.Results. The study of the antigenic activity of the test radiation antigen — plant quinoidr adiotoxin (QRT) — using the IHA-test showed that the test antigen has a high antigenic activity, inducing in the body of immunized animals (sheep) the synthesis of antiradiotoxic antibodies, the titers of which significantly exceed those immunized with liver (animal) antigen — animal radiotoxin. Quinoidr adiotoxin, obtained from plant tissue (potato tubers) and conjugated with a protein-lipoid group (incomplete Freund's adjuvant — IFA), has a high antigenicity, inducing the synthesis of specific antiradiotoxic antibodies, which can be used as an essential component of the immunochemical test system — sensitin used for sensitization of micro- and nanoparticles of bentonite in the design of antiradiation antibody variant of bentonited iagnosticum (ABBD).

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The authors declare that there are no conflicts of interest present.