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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vetpress</journal-id><journal-title-group><journal-title xml:lang="ru">Аграрная наука</journal-title><trans-title-group xml:lang="en"><trans-title>Agrarian science</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0869-8155</issn><issn pub-type="epub">2686-701X</issn><publisher><publisher-name>Редакция журнала "Аграрная наука"</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.32634/0869-8155-2023-368-3-46-51</article-id><article-id custom-type="elpub" pub-id-type="custom">vetpress-2537</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ЗООТЕХНИЯ И ВЕТЕРИНАРИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>ZOOTECHNICS AND VETERINARY MEDICINE</subject></subj-group></article-categories><title-group><article-title>Влияние Artemisiae absinthil herba и Inulae rhizomata et radices на процессы ферментации и метаногенез в рубце молодняка крупного рогатого скота</article-title><trans-title-group xml:lang="en"><trans-title>Influence of Artemisiae absinthil herba and Inulae rhizomata et radices  on fermentation processes and methanogenesis in the rumen of young cattle</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-2586-613X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Шейда</surname><given-names>Е. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Sheida</surname><given-names>E. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Елена Владимировна Шейда, кандидат биологических наукул. 9 Января, 29, Оренбург, 460000, Российская Федерацияпр. Победы, 13, Оренбург, 460018, Российская Федерация </p></bio><bio xml:lang="en"><p>Elena Vladimirovna Sheida, Candidate of Biological Sciences29 January 9 Str., Orenburg, 460000, Russian Federation13 Pobedy Ave., Orenburg, 460018, Russian Federation </p></bio><email xlink:type="simple">elena-shaejjda@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-0903-9561</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Рязанов</surname><given-names>В. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Ryazanov</surname><given-names>V. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Виталий Александрович Рязанов, кандидат сельскохозяйственных наук,ул. 9 Января, 29, Оренбург, 460000, Российская Федерация</p></bio><bio xml:lang="en"><p>Vitaly Aleksandrovich Ryazanov, Candidate of Agricultural Sciences29 January 9 Str., Orenburg, 460000, Russian Federation </p></bio><email xlink:type="simple">vita7456@yandex.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-9015-8367</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Дускаев</surname><given-names>Г. К.</given-names></name><name name-style="western" xml:lang="en"><surname>Duskaev</surname><given-names>G. K.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Галимжан Калиханович Дускаев, доктор биологических наук,ул. 9 Января, 29, Оренбург, 460000, Российская Федерация</p></bio><bio xml:lang="en"><p>Galimzhan Kalihanovich Duskaev, Doctor of Biological Sciences29 January 9 Str., Orenburg, 460000, Russian Federation </p></bio><email xlink:type="simple">gduskaev@mail.ru</email><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-0143-9499</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Рахматуллин</surname><given-names>Ш. Г.</given-names></name><name name-style="western" xml:lang="en"><surname>Rakhmatullin</surname><given-names>Sh. G.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Шамиль Гафиуллович Рахматуллин, кандидат биологических наукул. 9 Января, 29, Оренбург, 460000, Российская Федерация</p></bio><bio xml:lang="en"><p>Shamil Gafiullovich Rakhmatullin, Candidate of Biological Sciences29 January 9 Str., Orenburg, 460000, Russian Federation </p></bio><email xlink:type="simple">shahm2005@rambler.ru</email><xref ref-type="aff" rid="aff-4"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-0561-7002</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кван</surname><given-names>О. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Kvan</surname><given-names>O. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ольга Вилориевна Кван, кандидат биологических наукул. 9 Января, 29, Оренбург, 460000, Российская Федерацияпр. Победы, 13, Оренбург, 460018, Российская Федерация</p></bio><bio xml:lang="en"><p>Olga Vilorievna Kvan, Candidate of Biological Sciences29 January 9 Str., Orenburg, 460000, Russian Federation</p><p>13 Pobedy Ave., Orenburg, 460018, Russian Federation </p></bio><email xlink:type="simple">kwan111@yandex.ru</email><xref ref-type="aff" rid="aff-5"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Федеральный научный центр биологических систем и агротехнологий Российской академии наук;&#13;
Оренбургский государственный университет<country>Россия</country></aff><aff xml:lang="en">Federal Scientific Center of Biological Systems and Agrotechnologies of the Russian Academy of Sciences;&#13;
Orenburg State University<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru">Федеральный научный центр биологических систем и агротехнологий Российской академии наук<country>Россия</country></aff><aff xml:lang="en">Federal Scientific Center of Biological Systems and Agrotechnologies of the Russian Academy of Sciences<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru">Федеральный научный центр биологических систем&#13;
и агротехнологий Российской академии наук<country>Россия</country></aff><aff xml:lang="en">Federal Scientific Center of Biological Systems and Agrotechnologies of the Russian Academy of Sciences<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-4"><aff xml:lang="ru">Федеральный научный центр биологических систем и агротехнологий Российской академии наук<country>Россия</country></aff><aff xml:lang="en">Federal Scientific Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-5"><aff xml:lang="ru">Федеральный научный центр биологических систем и агротехнологий Российской академии наук;&#13;
Оренбургский государственный университет<country>Россия</country></aff><aff xml:lang="en">Federal Scientific Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences;&#13;
Orenburg State University<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2023</year></pub-date><pub-date pub-type="epub"><day>10</day><month>04</month><year>2023</year></pub-date><volume>0</volume><issue>3</issue><fpage>46</fpage><lpage>51</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Шейда Е.В., Рязанов В.А., Дускаев Г.К., Рахматуллин Ш.Г., Кван О.В., 2023</copyright-statement><copyright-year>2023</copyright-year><copyright-holder xml:lang="ru">Шейда Е.В., Рязанов В.А., Дускаев Г.К., Рахматуллин Ш.Г., Кван О.В.</copyright-holder><copyright-holder xml:lang="en">Sheida E.V., Ryazanov V.A., Duskaev G.K., Rakhmatullin S.G., Kvan O.V.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.vetpress.ru/jour/article/view/2537">https://www.vetpress.ru/jour/article/view/2537</self-uri><abstract><p>Актуальность. Растительные препараты и их биологически активные соединения могут улучшить использование корма и увеличить продуктивность животных за счет изменения микробной ферментации рубца. Проведена оценка влияния растительных препаратов — корневищ и корней девясила и травы полыни горькой на метаболические процессы в рубце и метаногенез.Методы. Для изучения использовали: Inulae rhizomata et radices в дозировках на 1 кг СВ: 3,0 г — 1-й образец, 1,0 г — 2-й образец, 6,0 г — 3-й образец; Artemisiae absinthil herba в дозировках на 1 кг СВ: 5,0 г — 4-й образец; 2,0 г — 5-й образец; 10,0 г — 6-й образец. Исследование проводили методом in vitro с использованием инкубатора ANKOM Daisy II (модификации D200 и D200I) по специализированной методике. Рубцовое содержимое получали от бычков казахской белоголовой породы с хронической фистулой рубца средней массой 220–225 кг в возрасте 9–10 месяцев. Отбор проб воздуха для определения уровня метана производили на приборе «Кристаллюкс-2000М» методом газовой хроматографии. Уровень летучих жирных кислот (ЛЖК) в содержимом рубца определяли методом газовой хроматографии на хроматографе газовом «Кристаллюкс-4000М», определение форм азота — по ГОСТ 26180-84.Результаты. Установлено, что различные дозировки растительных препаратов не оказывали существенного влияния на характеристики ферментации в ЖКТ. Трава полыни в дозировке 10,0 г на 1 кг СВ снижала выработку метана, чем другие ее дозировки (р ≤ 0,05). Так, доза 2,0 г показала повышение уровня метана в РЖ на 36,5% (р ≤ 0,01), доза 5,0 г — на 47,5% (р ≤ 0,01). Корневища и корни девясила показали высокий уровень выработки метана, однако min и max вводимые дозы данного фитобиотика показали снижение на 38,1–38,6%.</p></abstract><trans-abstract xml:lang="en"><p>Relevance. Herbal preparations and their biologically active compounds can improve the use of feed and increase the productivity of animals by changing the microbial fermentation of the rumen. In our work, we evaluated the effect of herbal preparations — rhizomes and roots of elecampane and wormwood herb, on metabolic processes in the rumen and methanogenesis.Methods. For the study: Inulae rhizomata et radices were used in dosages per 1 kg of CB: 3.0 g — 1st sample, 1.0 g — 2nd sample, 6.0 g — 3rd sample; Artemisiae absinthil herba in dosages per 1 kg of CB: 5.0 g — 4th sample, 2.0 g — 5th sample, 10.0 g — 6th sample. The study was carried out in vitro using the ANKOM Daisy II incubator (modifications D200 and D200I) according to a specialized technique. The scar content was obtained from Kazakh white-headed bulls with chronic scar fistula with an average weight of 220–225 kg at the age of 9–10 months. Air sampling to determine the level of methane was carried out on the device «Kristallux-2000M» by gas chromatography. The level of volatile fatty acids (VFA) in the contents of the scar was determined by gas chromatography on a gas chromatograph «Crystallux-4000M», determination of nitrogen forms — according to GOST 26180-84.Results. It was found that different dosages of herbal preparations did not significantly affect the characteristics of fermentation in the gastrointestinal tract. Wormwood grass at a dosage of 10.0 g per 1 kg of SV reduced methane production than its other dosages (p ≤ 0.05). Thus, a dose of 2.0 g showed an increase in the level of methane in rye by 36.5% (p ≤ 0.01), a dose of 5.0 g — by 47.5% (p ≤ 0.01). Rhizomes and roots of elecampane showed a high level of methane production, however, min and max administered doses of this phytobiotic showed a decrease of 38.1–38.6%.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>корни и корневища девясила</kwd><kwd>трава полыни горькой</kwd><kwd>азот</kwd><kwd>летучие жирные кислоты</kwd><kwd>метаногенез</kwd><kwd>метан</kwd><kwd>рубец</kwd><kwd>крупный рогатый скот</kwd></kwd-group><kwd-group xml:lang="en"><kwd>roots and rhizomes of elecampane</kwd><kwd>wormwood herb</kwd><kwd>nitrogen</kwd><kwd>volatile fatty acids</kwd><kwd>methanogenesis</kwd><kwd>methane</kwd><kwd>scar</kwd><kwd>cattle</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Lassey K.R. Livestock methane emission and its perspective in the global methane cycle. Aust J. Exp Agr. 2008; 48: 114–118. DOI:10.1071/EA07220</mixed-citation><mixed-citation xml:lang="en">Lassey K.R. Livestock methane emission and its perspective in the global methane cycle. Aust J. Exp Agr. 2008; 48: 114–118. DOI:10.1071/EA07220</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Johnson K.A., Johnson D.E. Methane emissions from cattle. J. Anim Sci. 1995; 73(95): 2483–2492. 630–638. DOI: 10.1111/jpn.12842</mixed-citation><mixed-citation xml:lang="en">Johnson K.A., Johnson D.E. Methane emissions from cattle. J. Anim Sci. 1995; 73(95): 2483–2492. 630–638. DOI: 10.1111/jpn.12842</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Bodas R. et al. In vitro screening of the potential of numerous plant species as antimethanogenic feed additives for ruminants. Anim Feed Sci Technol. 2008; 145(1–4): 245–258. https://www.sciencedirect.com/science/article/abs/pii/S0377840107002763</mixed-citation><mixed-citation xml:lang="en">Bodas R. et al. In vitro screening of the potential of numerous plant species as antimethanogenic feed additives for ruminants. Anim Feed Sci Technol. 2008; 145(1–4): 245–258. https://www.sciencedirect.com/science/article/abs/pii/S0377840107002763</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Chesson A., Stewart C.S., Wallace R.J. Influence of plant phenolic acids on growth and cellulolytic activity of rumen bacteria. Appl Environ Microbiol. 1982; 44: 597–603. DOI: 10.1128/aem.44.3.597-603.1982</mixed-citation><mixed-citation xml:lang="en">Chesson A., Stewart C.S., Wallace R.J. Influence of plant phenolic acids on growth and cellulolytic activity of rumen bacteria. Appl Environ Microbiol. 1982; 44: 597–603. DOI: 10.1128/aem.44.3.597-603.1982</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Chattopadhyay M.K. Use of antibiotics as feed additives: A burning question. Frontiers in Microbiology. 2014; 5: 1–3. https://doi.org/10.3389/fmicb.2014.00334</mixed-citation><mixed-citation xml:lang="en">Chattopadhyay M.K. Use of antibiotics as feed additives: A burning question. Frontiers in Microbiology. 2014; 5: 1–3. https://doi.org/10.3389/fmicb.2014.00334</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Khiaosa-ard R., Zebeli Q. Meta-analysis of the effects of essential oils and their bioactive compounds on rumen fermentation characteristics and feed efficiency in ruminants. J. Anim Sci. 2013; 91: 1819–1830. https://doi.org/10.2527/jas.2012-5691</mixed-citation><mixed-citation xml:lang="en">Khiaosa-ard R., Zebeli Q. Meta-analysis of the effects of essential oils and their bioactive compounds on rumen fermentation characteristics and feed efficiency in ruminants. J. Anim Sci. 2013; 91: 1819–1830. https://doi.org/10.2527/jas.2012-5691</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Devant M., Anglada A., Bach A. Effects of plant extract supplementation on rumen fermentation and metabolism in young Holstein bulls consuming high levels of concentrate. Animal Feed Science and Technology. 2007; 137(1–2): 46–57. https://doi.org/10.1016/j.anifeedsci.2006.10.003</mixed-citation><mixed-citation xml:lang="en">Devant M., Anglada A., Bach A. Effects of plant extract supplementation on rumen fermentation and metabolism in young Holstein bulls consuming high levels of concentrate. Animal Feed Science and Technology. 2007; 137(1–2): 46–57. https://doi.org/10.1016/j.anifeedsci.2006.10.003</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Bampidis V.A., Christodoulou V., Florou-Paneri P., Christaki E. Effect of dried oregano leaves versus neomycin in treating newborn calves with colibacillosis. Journal of Veterinary Medicine Series A. 2006; 53: 154–156. https://doi.org/10.1111/j.1439-0442.2006.00806.x</mixed-citation><mixed-citation xml:lang="en">Bampidis V.A., Christodoulou V., Florou-Paneri P., Christaki E. Effect of dried oregano leaves versus neomycin in treating newborn calves with colibacillosis. Journal of Veterinary Medicine Series A. 2006; 53: 154–156. https://doi.org/10.1111/j.1439-0442.2006.00806.x</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Akbarian-Tefaghi M., Ghasemi E., Khorvash M. Performance, rumen fermentation and blood metabolites of dairy calves fed starter mixtures supplemented with herbal plants, essential oils or monensin. J. Anim Physiol Anim Nutr (Berl). 2018; 102(3): 630–638. DOI: 10.1111/jpn.12842</mixed-citation><mixed-citation xml:lang="en">Akbarian-Tefaghi M., Ghasemi E., Khorvash M. Performance, rumen fermentation and blood metabolites of dairy calves fed starter mixtures supplemented with herbal plants, essential oils or monensin. J. Anim Physiol Anim Nutr (Berl). 2018; 102(3): 630–638. DOI: 10.1111/jpn.12842</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Calsamiglia S., Busquet M., Cardozo P.W., Castillejos L., Ferret A. Invited review: Essential oils as modifiers of rumen microbial fermentation. Journal of Dairy Science. 2007; 90: 2580–2595. https://doi.org/10.3168/jds.2006-644</mixed-citation><mixed-citation xml:lang="en">Calsamiglia S., Busquet M., Cardozo P.W., Castillejos L., Ferret A. Invited review: Essential oils as modifiers of rumen microbial fermentation. Journal of Dairy Science. 2007; 90: 2580–2595. https://doi.org/10.3168/jds.2006-644</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Franz C.K., Baser H.C., Windisch W. Essential oils and aromatic plants in animal feeding a European perspective: A review. Flavour and Fragrance Journal. 2010; 25(5): 327–340. https://doi.org/10.1002/ffj.1967</mixed-citation><mixed-citation xml:lang="en">Franz C.K., Baser H.C., Windisch W. Essential oils and aromatic plants in animal feeding a European perspective: A review. Flavour and Fragrance Journal. 2010; 25(5): 327–340. https://doi.org/10.1002/ffj.1967</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Нормы и рационы кормления сельскохозяйственных животных. Справочное пособие. 3-е издание переработанное и дополненное / под ред. А.П. Калашникова, В.И. Фисинина, В.В. Щеглова, Н.И. Клейменова. Москва: Издательство Знание. 2003; 456. eLIBRARY ID: 18902061</mixed-citation><mixed-citation xml:lang="en">Norms and rations of farm animals. Reference manual, 3rd edition revised and supplemented / edited by A.P. Kalashnikov, V.I. Fisinin, V.V. Shcheglova, N.I. Kleymenovo. Moscow: Publishing house «Knowledge» (Izdatelstvo «Znaniye»). 2003; 456. eLIBRARY ID: 18902061 (In Russian).</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Кузнецов В.М. Основы научных исследований в животноводстве. Киров: Зональный НИИСХ Северо-Востока. 2006; 568.</mixed-citation><mixed-citation xml:lang="en">Kuznetsov V.M. Fundamentals of scientific research in animal husbandry. Kirov: Zonal Research Institute of Agriculture of the North-East. 2006; 568. (In Russian).</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Султанаева Л.З., Балджи Ю.А. Эффективность использования фитобиотических добавок в рационе крупного и мелкого рогатого скота (обзор). Животноводство и кормопроизводство. 2021; 2(104): 96–110. DOI: 10.33284/2658-3135-104-2-96</mixed-citation><mixed-citation xml:lang="en">Sultanaeva L.Z., Baldzhi Yu.A. The efficiency of the use of phytobiotic additives in the diet of large and small cattle (review). Animal husbandry and fodder production (Zhivotnovodstvo i kormoproizvodstvo). 2021; 2(104): 96–110. DOI: 10.33284/2658-3135-104-2-96 (In Russian).</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Левахин Г.И., Мещеряков А.Г. К методике определения расщепляемости протеина кормов в лабораторных условиях. Доклады Российской академии сельскохозяйственных наук. 2003; 3: 12–13. eLIBRARY ID: 18029721</mixed-citation><mixed-citation xml:lang="en">Levakhin G.I., Meshcheryakov A.G. To the method for determining the digestibility of feed protein in laboratory conditions. Reports of the Russian Academy of Agricultural Sciences (Russian Agricultural Sciences). 2003; 3: 12–13. eLIBRARY ID: 18029721 (In Russian).</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Paiva P.M.G., Gomes F.S., Napoleão T.H., Sá R.A., Correia M.T.S., Coelho L.C.B. Antimicrobial Activity of Secondary Metabolites and Lectins from Plants. In book: Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology. Edition: 01 Chapter: Antimicrobial activity of secondary metabolites and lectins from plants. Editors: A. Méndez-Villas. Publisher: FORMATEX. 2010; 396–406.</mixed-citation><mixed-citation xml:lang="en">Paiva P.M.G., Gomes F.S., Napoleão T.H., Sá R.A., Correia M.T.S., Coelho L.C.B. Antimicrobial Activity of Secondary Metabolites and Lectins from Plants. In book: Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology. Edition: 01 Chapter: Antimicrobial activity of secondary metabolites and lectins from plants. Editors: A. Méndez-Villas. Publisher: FORMATEX. 2010; 396–406.</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Castillejos L., Calsamiglia S., Ferret A. Effect of essential oils active compounds on rumen microbial fermentation and nutrient flow in vitro systems. Journal of Dairy Science. 2006; 89(7): 2649–2658. https://doi.org/10.3168/jds.S0022-0302(06)72341-4</mixed-citation><mixed-citation xml:lang="en">Castillejos L., Calsamiglia S., Ferret A. Effect of essential oils active compounds on rumen microbial fermentation and nutrient flow in vitro systems. Journal of Dairy Science. 2006; 89(7): 2649–2658. https://doi.org/10.3168/jds.S0022-0302(06)72341-4</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Cardozo P.W., Calsamiglia S., Ferret A., Kamel C. Screening for the effects of natural plant extracts atdifferent pH on in vitro rumen microbial fermentation of a high concentrate diet for beef cattle. Journal of Animal Science. 2005; 83(11): 2572–2579. https://doi.org/10.2527/2005.83112572x</mixed-citation><mixed-citation xml:lang="en">Cardozo P.W., Calsamiglia S., Ferret A., Kamel C. Screening for the effects of natural plant extracts atdifferent pH on in vitro rumen microbial fermentation of a high concentrate diet for beef cattle. Journal of Animal Science. 2005; 83(11): 2572–2579. https://doi.org/10.2527/2005.83112572x</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">McIntosh F.M., Williams P., Losa R., Wallace R.J., Beever D.A., Newbold C.J. Effects of essential oils on ruminal microorganisms and their protein metabolism. Applied Environmental Microbiology. 2003; 69: 5011–5014. https://doi.org/10.1128/AEM.69.8.5011-5014.2003</mixed-citation><mixed-citation xml:lang="en">McIntosh F.M., Williams P., Losa R., Wallace R.J., Beever D.A., Newbold C.J. Effects of essential oils on ruminal microorganisms and their protein metabolism. Applied Environmental Microbiology. 2003; 69: 5011–5014. https://doi.org/10.1128/AEM.69.8.5011-5014.2003</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Guo Y.Q., Liu J.X., Lu Y., Zhu W.Y., Denman S.E., McSweeney C.S. Effect of tea saponin on methanogenesis, microbial community structure and expression of mcrA gene, in cultures of rumen micro-organisms. Lett Appl Microbiology. 2008; 47(5): 421–426. DOI: 10.1111/j.1472-765X.2008.02459.x</mixed-citation><mixed-citation xml:lang="en">Guo Y.Q., Liu J.X., Lu Y., Zhu W.Y., Denman S.E., McSweeney C.S. Effect of tea saponin on methanogenesis, microbial community structure and expression of mcrA gene, in cultures of rumen micro-organisms. Lett Appl Microbiology. 2008; 47(5): 421–426. DOI: 10.1111/j.1472-765X.2008.02459.x</mixed-citation></citation-alternatives></ref><ref id="cit21"><label>21</label><citation-alternatives><mixed-citation xml:lang="ru">Holtshausen L. et all. Feeding saponin-containing Yucca schidigera and Quillaja saponaria to decrease enteric methane production in dairy cows. Journal of Dairy Science. 2009; 92(6): 2809–2821. https://doi.org/10.3168/jds.2008-1843</mixed-citation><mixed-citation xml:lang="en">Holtshausen L. et all. Feeding saponin-containing Yucca schidigera and Quillaja saponaria to decrease enteric methane production in dairy cows. Journal of Dairy Science. 2009; 92(6): 2809–2821. https://doi.org/10.3168/jds.2008-1843</mixed-citation></citation-alternatives></ref><ref id="cit22"><label>22</label><citation-alternatives><mixed-citation xml:lang="ru">Beauchemin К.А., McGinn S.M. Methane emissions from beef cattle: effects of fumaric acid, essential oil, and canola oil. Anim Sci. 2006; 84(6): 1489–1496. doi: 10.2527/2006.8461489x.</mixed-citation><mixed-citation xml:lang="en">Beauchemin К.А., McGinn S.M. Methane emissions from beef cattle: effects of fumaric acid, essential oil, and canola oil. Anim Sci. 2006; 84(6): 1489–1496. doi: 10.2527/2006.8461489x.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
