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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vetpress</journal-id><journal-title-group><journal-title xml:lang="ru">Аграрная наука</journal-title><trans-title-group xml:lang="en"><trans-title>Agrarian science</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0869-8155</issn><issn pub-type="epub">2686-701X</issn><publisher><publisher-name>Редакция журнала "Аграрная наука"</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.32634/0869-8155-2023-369-4-57-61</article-id><article-id custom-type="elpub" pub-id-type="custom">vetpress-2578</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ЗООТЕХНИЯ И ВЕТЕРИНАРИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>ZOOTECHNICS AND VETERINARY MEDICINE</subject></subj-group></article-categories><title-group><article-title>Полиморфные варианты генов CD62L и ACSL1 в связи с устойчивостью коров к маститу</article-title><trans-title-group xml:lang="en"><trans-title>Polymorphic variants of the CD62L and ACSL1 genes in connection with the resistance of cows to mastitis</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-8658-2026</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Позовникова</surname><given-names>М. B.</given-names></name><name name-style="western" xml:lang="en"><surname>Pozovnikova</surname><given-names>M. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Марина Владимировна Позовникова, кандидат биологических наук</p><p>Московское шоссе, д. 55А, Санкт-Петербург, Пушкин, 196601</p></bio><bio xml:lang="en"><p>Marina Vladimirovna Pozovnikova, candidate of biological sciences</p><p>55А  Moskovskoe shosse, St. Petersburg, Pushkin, 196601</p></bio><email xlink:type="simple">pozovnikova@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-4225-5533</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Романова</surname><given-names>Е. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Romanova</surname><given-names>E. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Елена Анатольевна Романова, младший научный сотрудник</p><p>Московское шоссе, д. 55А, Санкт-Петербург, Пушкин, 196601</p></bio><bio xml:lang="en"><p>Elena Anatolievna Romanova, junior researcher</p><p>55А  Moskovskoe shosse, St. Petersburg, Pushkin, 196601</p></bio><email xlink:type="simple">splicing86@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Тулинова</surname><given-names>О. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Tulinova</surname><given-names>O. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ольга Васильевна Тулинова, кандидат сельскохозяйственных наук</p><p>Московское шоссе, д. 55А, Санкт-Петербург, Пушкин, 196601</p></bio><bio xml:lang="en"><p>Olga Vasilevna Tulinova, Candidate of Agricultural Sciences</p><p>55А  Moskovskoe shosse, St. Petersburg, Pushkin, 196601</p><p> </p></bio><email xlink:type="simple">tulinova_59@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Всероссийский научно-исследовательский институт генетики и разведения сельскохозяйственных животных — филиал Федерального исследовательского центра животноводства — ВИЖ им. академика Л.К. Эрнста</institution><country>Россия</country></aff><aff xml:lang="en"><institution>The All-Russian Research Institute of Genetics and Breeding of Farm Animals is a branch of the Federal Research Center of Animal Husbandry — VIZ Academician L.K. Ernst</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2023</year></pub-date><pub-date pub-type="epub"><day>10</day><month>05</month><year>2023</year></pub-date><volume>0</volume><issue>4</issue><fpage>57</fpage><lpage>61</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Позовникова М.B., Романова Е.А., Тулинова О.В., 2023</copyright-statement><copyright-year>2023</copyright-year><copyright-holder xml:lang="ru">Позовникова М.B., Романова Е.А., Тулинова О.В.</copyright-holder><copyright-holder xml:lang="en">Pozovnikova M.V., Romanova E.A., Tulinova O.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.vetpress.ru/jour/article/view/2578">https://www.vetpress.ru/jour/article/view/2578</self-uri><abstract><sec><title>Актуальность</title><p>Актуальность. В условиях интенсивного ведения молочного скотоводства заболеваемость коров маститом продолжает оставаться актуальной проблемой. Количество соматических клеток (КСК) в молоке является селекционным показателем для улучшения состояния здоровья молочной железы коров. Изучение резистентности животных к заболеванию, в основе которой в том числе лежит и генетическая составляющая, позволит в перспективе повысить эффективность селекции. Цель работы — сравнительный анализ связи полиморфных вариантов генов ACS L1 (rs208522533) и CD62L (rs41803917 и rs41803917) с показателями молочной продуктивности и уровнем соматических клеток в молоке коров айрширской породы.</p></sec><sec><title>Методы</title><p>Методы. Сформирована выборка коров-первотелок айрширской породы (n = 191), принадлежащих одному из племенных хозяйств Ленинградской области. Генотипы животных определяли методом ПЦР-ПДРФ. Анализ частоты генотипов по rs208522533 гена ACSLI показал, что 99% животных были носителями генотипа GG.</p></sec><sec><title>Результаты</title><p>Результаты. Рeзультаты исследований гена CD62L показали, что по rs41803917 определена высокая частота аллеля G (80,4%) и в среднем более 60% животных имели генотип GG. По rs109966956 гена CD62L выявлена высокая частота аллеля С (80,1%) и 64,9% животных имели генотип СС. Установлены высокие значения ПЦ по БОКСК в малочисленных группах животных с генотипом АА по rs41803917 (р £ 0,05) и генотипом ТТ по rs109966956 (р £ 0,001). Особи с генотипом AG по rs41803917 гена CD62L имели высокие показатели процентного содержания белка (р £ 0,05) и низким БОКСК (р £ 0,05). Полученные результаты свидетельствуют о том, что SNPs rs41803917 и rs41803917 гена CD62L можно рассматривать как потенциальные маркеры резистентности к маститу у айрширских коров.</p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Relevance</title><p>Relevance. In conditions of intensive dairy cattle breeding, the incidence of cows with mastitis continues to be an urgent problem. The number of somatic cells (CCCs) in milk is a breeding indicator for improving the health of the mammary gland of cows. The study of animal resistance to the disease, which is based, among other things, on the genetic component, will make it possible to increase the efficiency of breeding in the future. The purpose of this work is a comparative analysis of the relationship of polymorphic variants of the ACS L1 (rs208522533) and CD62L (rs41803917 and rs41803917) genes with indicators of milk productivity and the level of somatic cells in the milk of Ayrshire cows.</p></sec><sec><title>Methods</title><p>Methods. A sample of first-calf cows of Ayrshire breed (n = 191) belonging to one of the breeding farms of the Leningrad region was formed. Animal genotypes were determined by PCR-PDRF. Analysis of the genotype frequency by rs208522533 of the ACS LI gene showed that 99% of the animals were carriers of the GG genotype.</p></sec><sec><title>Results</title><p>Results. The results of studies of the CD62L gene showed that rs41803917 determined a high frequency of the G allele (80.4%) and on average more than 60% of animals had the GG genotype. rs109966956 of the CD62L gene revealed a high frequency of the C allele (80.1%) and 64.9% of the animals had the CC genotype. High values of BOX PC were established in small groups of animals with the AA genotype according to rs41803917 (p £ 0.05) and the TT genotype according to rs109966956 (p £ 0.001). Individuals with the AG genotype according to rs41803917 of the CD62L gene had high protein percentages (p £ 0.05) and low BSC (p £ 0.05). The results obtained indicate that SNPs rs41803917 and rs41803917 of the CD62L gene can be considered as potential markers of resistance to mastitis in Ayrshire cows.</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>айрширская порода</kwd><kwd>количество соматических клеток</kwd><kwd>полиморфизм</kwd><kwd>SNP</kwd></kwd-group><kwd-group xml:lang="en"><kwd>аyrshire breed</kwd><kwd>somatic cell count</kwd><kwd>polymorphism</kwd><kwd>SNP</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Работа проведена в рамках выполнения научных исследований Министерства науки и высшего образования РФ по теме № 121052600344-8.</funding-statement><funding-statement xml:lang="en">The work was funded by the Ministry of Science and Higher Education of the Russian Federation by topic No. 121052600344-8.</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Cheng W.N., Han S.G. 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