vetpressАграрная наукаAgrarian science0869-81552686-701XРедакция журнала "Аграрная наука"10.32634/0869-8155-2023-370-5-27-32vetpress-2614Research ArticleЗООТЕХНИЯ И ВЕТЕРИНАРИЯZOOTECHNICS AND VETERINARY MEDICINEИспользование проб молока при эпизоотическом контроле болезней крупного рогатого скотаUse of milk samples in epizootic surveillance of cattle diseaseshttps://orcid.org/0000-0002-9752-6337МищенкоА. В.MishchenkoA. V.

Алексей Владимирович Мищенко, доктор ветеринарных наук, Рязанский пр-т, д. 24, к. 1, Москва, 109428;

пос. Биокомбината, 24, Московская обл., 141142

Aleksey Vladimirovich Mishchenko, Doctor of Veterinary Sciences, 24 Ryazan Ave., 1 building, Moscow, 109428;

24 village of Biocombinata, Moscow region, 141142

studebaker@yandex.ruhttps://orcid.org/0000-0003-2160-4770ГулюкинА. М.GulyukinA. M.

Алексей Михайлович Гулюкин, доктор ветеринарных наук, член-корреспондент РАН, директор,

Рязанский пр-т, д. 24, к. 1, Москва, 109428

Aleksey Mikhailovich Gulyukin, Doctor of Veterinary Sciences, Corresponding Member of the Russian Academy of Sciences, Director, 

24 Ryazan Ave., 1 building, Moscow, 109428

admin@viev.ruhttps://orcid.org/0000-0002-0061-5799ОганесянА. С.OganesyanA. S.

Андрей Серожович Оганесян, кандидат ветеринарных наук, заведующий сектором анализа риска,

мкр. Юрьевец, Владимир, 600901

Andrey Serozhovich Oganesyan, Candidate of Veterinary Sciences, Head of the Risk Analysis Sector, 

Yurievets, Vladimir, 600901

oganesyan@arriah.ruhttps://orcid.org/0000-0003-3751-2168МищенкоВ. А.MishchenkoV. A.

Владимир Александрович Мищенко, доктор ветеринарных наук, профессор, главный научный сотрудник,

Рязанский пр-т, д. 24, к. 1, Москва, 109428

Vladimir Alexandrovich Mishchenko, Doctor of Veterinary Sciences, Professor, Chief Researcher, 

24 Ryazan Ave., 1 building, Moscow, 109428

mishenko@arriah.ruhttps://orcid.org/0000-0002-7489-6175ГулюкинМ. И.GulyukinM. I.

Михайл Иванович Гулюкин, доктор ветеринарных наук, профессор, академик РАН,

Рязанский пр-т, д. 24, к. 1, Москва, 109428

Mikhail Ivanovich Gulyukin, Doctor of Veterinary Sciences, Professor, Academician of the Russian Academy of Sciences, 

24 Ryazan Ave., 1 building, Moscow, 109428

admin@viev.ruhttps://orcid.org/0000-0003-3201-1065ЛопуновС. В.LopunovS. V.

Сергей Витальевич Лопунов, кандидат ветеринарных наук, старший научный сотрудник,

Рязанский пр-т, д. 24, к. 1, Москва, 109428

Sergey Vitalievich Lopunov, Candidate of Veterinary Sciences, Senior Researcher, 

24 Ryazan Ave., 1 building, Moscow, 109428

admin@viev.ruhttps://orcid.org/0000-0002-7173-5501ЗаболотнаяИ. М.ZabolotnayaI. M.

Ирина Михайловна Заболотная, кандидат биологических наук, старший научный сотрудник,

Рязанский пр-т, д. 24, к. 1, Москва, 109428

Irina Mikhailovna Zabolotnaya, Candidate of Biological Sciences, Senior Researcher,

24 Ryazan Ave., 1 building, Moscow, 109428

admin@viev.ruВсероссийский научно-исследовательский институт экспериментальной ветеринарии им. К.И. Скрябина и Я.Р. Коваленко; Всероссийский научно-исследовательский и технологический институт биологической промышленностиРоссияFederal Scientific Centre VIEV; All-Russian Research and Technological Institution of Biological Industry (VNITIBP)Russian FederationВсероссийский научно-исследовательский институт экспериментальной ветеринарии им. К.И. Скрябина и Я.Р. КоваленкоРоссияFederal Scientific Centre VIEVRussian FederationФедеральный центр охраны здоровья животныхРоссияFederal Centre for Animal HealthRussian FederationВсероссийский научно-исследовательский институт экспериментальной ветеринарии им. К.И. Скрябина и Я.Р. Коваленко; Федеральный центр охраны здоровья животныхРоссияFederal Scientific Centre VIEV; Federal Centre for Animal HealthRussian Federation202304062023052732Copyright © Мищенко А.В., Гулюкин А.М., Оганесян А.С., Мищенко В.А., Гулюкин М.И., Лопунов С.В., Заболотная И.М., 20232023Мищенко А.В., Гулюкин А.М., Оганесян А.С., Мищенко В.А., Гулюкин М.И., Лопунов С.В., Заболотная И.М.Mishchenko A.V., Gulyukin A.M., Oganesyan A.S., Mishchenko V.A., Gulyukin M.I., Lopunov S.V., Zabolotnaya I.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vetpress.ru/jour/article/view/2614Актуальность

Актуальность. Приведен анализ возможности использования молока в качестве неинвазивного типа проб при эпизоотологическом контроле заболеваний крупного рогатого скота. В процессе патогенеза многие возбудители вызывают поражения молочной железы или выделяются вместе с молоком, что делает молоко идеальной пробой для лабораторной диагностики инфекционных болезней крупного рогатого скота, поскольку оно доступно в любом количестве и его пробы легко собрать.

Методы

Методы. Использованы общепринятые методы анализа документов.

Результаты

Результаты. Показано, что пробы молока возможно использовать как на индивидуальном, так и на популяционном уровне для ранней идентификации инфицированных стад, проводить скрининг инфицированных стад и использовать для получения доказательств благополучия стад. Доступность коммерческих диагностических тест-систем для выявления антител в молоке к возбудителям лейкоза КРС, вирусной диареи КРС, Brucella abortus, Mycobacterium avium subspecies paratuberculosis, Fasciola hepatica, Ку-лихорадки (Coxiella burnetii) делает доступными программы контроля и искоренения болезней в дойных стадах и на уровне целых стран. Использование объединенных неинвазивных проб молока позволяет вести борьбу с медленно прогрессирующими и хроническими инфекциями дойного скота (лейкоз КРС, паратуберкулез КРС, бруцеллез) и в режиме реального времени исключать животных-носителей из производственной цепочки, что свидетельствует о целесообразности внедрения данного вида проб в ветеринарную практику в Российской Федерации.

Relevance

Relevance. The analysis of the possibility of using milk as a non-invasive type of samples in the epizootological control of diseases of cattle is given. During pathogenesis, many etiologic agents cause breast lesions or are excreted together with milk, which makes milk an ideal sample for laboratory diagnostics of infectious diseases of cattle, since it is available in any quantity and its samples are easy to collect.

Methods

Methods. Conventional methods of document analysis were used.

Results

Results. It is shown that milk samples can be used both at the individual and at the population level for early identification of infected herds, screening of infected herds and use to obtain evidence of the well-being of herds. The availability of commercial diagnostic test systems for detecting antibodies in milk to the causative agents of leukemia of cattle, viral diarrhea of cattle, Brucella abortus, Mycobacterium avium subspecies paratuberculosis, Fasciola hepatica, Q fever (Coxiella burnetii) makes available programs for the control and eradication of diseases in dairy herds and at the level of countries. The use of combined non-invasive milk samples makes it possible to combat slowly progressive and chronic cattle infections of dairy cattle (bovine leucosis, paratuberculosis, brucellosis), and exclude carrier animals from the production chain in real time, which indicates the feasibility of introducing this type of samples into veterinary practice in the Russian Federation.

вирус вирусной диареи крупного рогатого скотавирус энзоотического лейкоза крупного рогатого скотавирус инфекционного ринотрахеита крупного рогатого скотаBrucella abortusкрупный рогатый скотмелкий рогатый скотмолокоиммуноферментный анализполимеразная цепная реакцияскринингмониторингbovine viral diarrhea virusbovine enzootic leukemia virusinfectious bovine rhinotracheitis virusBrucella abortuscattlesmall ruminantsmilkenzyme-linked immunosorbent assaypolymerase chain reactionscreeningmonitoringРабота проведена в рамках выполнения государственных заданий № FGUG-2022-0007 и FGUG-2022-0009.The work was carried out as part of the implementation of state assignments No. FGUG-2022-0007 and FGUG-2022-0009.ReferencesЧугаева Н.А., Мищенко А.В., Кондаков С.Э., Белецкий С.О., Гордеев В.В., Дроздова Е.И. Перспективы использования технологии ДБС (сухие пятна крови) в ветеринарии. 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The authors declare that there are no conflicts of interest present.