vetpressАграрная наукаAgrarian science0869-81552686-701XРедакция журнала "Аграрная наука"10.32634/0869-8155-2023-372-7-40-47vetpress-2697Research ArticleЗООТЕХНИЯ И ВЕТЕРИНАРИЯZOOTECHNICS AND VETERINARY MEDICINEПрименение фитобиотиков в кормлении рыб в качестве альтернативы антибактериальным и пробиотическим препаратам (обзор)The use of phytobiotics in fish feeding as an alternative to antibacterial and probiotic preparations (review)https://orcid.org/0000-0003-3804-5151МирошниковаЕ. П.MiroshnikovaE. P.

Елена Петровна Мирошникова, доктор биологических наук, профессор, заведующая кафедрой биотехнологии животного сырья и аквакультуры,

пр-т Победы, 13, корп. 20, Оренбург, 460018

Elena Petrovna Miroshnikova, Doctor of Biological Sciences, Professor, Head of the Department of Biotechnology of Animal Raw Materials and Aquaculture,

13 Pobedy Ave., 20 building, Orenburg, 460018

elenaakva@rambler.ruhttps://orcid.org/0000-0001-6534-7118АринжановА. Е.ArinzhanovA. E.

Азамат Ерсаинович Аринжанов, кандидат сельскохозяйственных наук, доцент кафедры биотехнологии животного сырья и аквакультуры,

пр-т Победы, 13, корп. 20, Оренбург, 460018

Azamat Ersainovich Arinzhanov, Candidate of Agricultural Sciences, Associate Professor of the Department of Biotechnology of Animal Raw Materials and Aquaculture,

 13 Pobedy Ave., 20 building, Orenburg, 460018

arin.azamat@mail.ruhttps://orcid.org/0000-0002-2385-264XКиляковаЮ. В.KilyakovaYu. V.

Юлия Владимировна Килякова, кандидат биологических наук, доцент кафедры биотехнологии животного сырья и аквакультуры,

пр-т Победы, 13, корп. 20, Оренбург, 460018

Yulia Vladimirovna Kilyakova, Candidate of Biological Sciences, Associate Professor of theDepartment of Biotechnology of Animal Raw Materials and Aquaculture,

13 Pobedy Ave., 20 building, Orenburg, 460018

fish-ka06@mail.ruhttps://orcid.org/0000-0003-1099-3117СизенцовА. Н.SizentsovA. N.

Алексей Николаевич Сизенцов, кандидат биологических наук, доцент кафедры биохимии и микробиологии,

пр-т Победы, 13, корп. 20, Оренбург, 460018

Alexey Nikolaevich Sizentsov, Candidate of Biological Sciences, Associate Professor of theDepartment of Biochemistry and Microbiology,

13 Pobedy Ave., 20 building, Orenburg, 460018

kwan111@yandex.ruОренбургский государственный университетРоссияOrenburg State UniversityRussian Federation202325072023074047Copyright © Мирошникова Е.П., Аринжанов А.Е., Килякова Ю.В., Сизенцов А.Н., 20232023Мирошникова Е.П., Аринжанов А.Е., Килякова Ю.В., Сизенцов А.Н.Miroshnikova E.P., Arinzhanov A.E., Kilyakova Y.V., Sizentsov A.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vetpress.ru/jour/article/view/2697Актуальность

Актуальность. Использование антибактериальных препаратов в качестве кормовых добавок на протяжении многих лет позволяло снижать уровни риска развития инфекционных патологий и, как следствие, повышало продуктивность отрасли, помимо очевидных преимуществ, постоянное использование антибиотиков развивает бактериальную резистентность, негативно влияет на представителей нормофлоры биоценозов и обладает аккумулирующим эффектом в аквакультуре. Одним из возможных направлений повышения продуктивности является использование пробиотических штаммов с выраженными антагонистическими характеристиками в отношении патогенных микроорганизмов. Следует отметить, что для оптимального роста рыбы необходим высокий уровень видоспецифичности структурного микробиома, что в конечном итоге не может гарантировать эффективность применения коммерческих пробиотических препаратов в рационах кормления рыб.

Результаты

Результаты. Представленные научные данные по использованию фитобиотиков свидетельствуют не только о выраженном стимулирующем рост рыб действии, но также и о высоком антиоксидантном и иммуностимулирующем действии. Отдельно стоит отметить высокий уровень эффективности тестируемых растений в отношении патогенных штаммов микроорганизмов A. hydrophila (порошок листьев портулака; экстракт плодов кизила; комбинация эфирных масел тимьяна, красного тимьяна и перца розмарина; пищевые наночастицы корицы, комбинация экстрактов кориандра, мальвы обыкновенной, дубового желудя; экстракт пандана кровельного), A. veronii (порошок косточек абрикоса), L. monocytogenes (экстракт листьев базилика гвоздичного), S. agalactiae (экстракт плодов айвы бельгийской). Гипотетически данный механизм можно объяснить не только активацией показателей неспецифического иммунитета, но и влиянием большинства тестируемых в экспериментах растений анти Quorum Sensing эффектом. Данный обзор направлен на описание мировой тенденции использования фитобиотиков в кормлении рыб.

Relevance

Relevance. The use of antibacterial drugs as feed additives for many years has made it possible to reduce the risk of developing infectious pathologies and, as a result, increased the productivity of the industry, in addition to obvious advantages, the constant use of antibiotics develops bacterial resistance, negatively affects representatives of the normoflora of biocenoses and has an accumulating effect in aquaculture. One of the possible ways to increase productivity is the use of probiotic strains with pronounced antagonistic characteristics against pathogenic microorganisms. It should be noted that for optimal fish growth, a high level of species-specificity of the structural microbiome is necessary, which ultimately cannot guarantee the effectiveness of the use of commercial probiotic drugs in fish feeding diets.

Results

Results. The presented scientific data on the use of phytobiotics indicate not only a pronounced effect stimulating the growth of fish, but also a high antioxidant and immunostimulating effect. Separately, it is worth noting the high level of effectiveness of the tested plants against pathogenic strains of A. hydrophila microorganisms (purslane leaf powder; dogwood fruit extract; a combination of essential oils of thyme, red thyme and rosemary pepper; food nanoparticles of cinnamon, a combination of extracts of coriander, mallow, oak acorn; pandanus roofing extract), A. veronii (apricot seed powder), L. monocytogenes (basil clove leaf extract), S. agalactiae (Belgian quince fruit extract). Hypothetically, this mechanism can be explained not only by the activation of indicators of nonspecific immunity, but also by the influence of the majority of plants tested in experiments with the anti Quorum Sensing effect. This review is aimed at describing the global trend in the use of phytobiotics in fish feeding.

фитобиотикикормление рыбпрофилактика инфекционных заболеванийантибиотикипробиотикилитературный обзорphytobioticsfish feedingprevention of infectious diseasesantibioticsprobioticsliterature reviewМатериалы подготовлены в рамках конкурса Российского научного фонда 2022 года «Проведение фундаментальных научных исследований и поисковых научных исследований малыми научными группами» (Соглашение от 27.12.2021 № 22-26-00281).The materials were prepared as part of the 2022 competition of the Russian Science Foundation «Conducting fundamental scientific research and exploratory scientific research by small scientific groups» (Agreement of 27.12.2021 No. 22-26-00281).ReferencesZhou L., Mchele Limbu S., Qiao F., Du Z.Y., Zhang M. Influence of Long-Term Feeding Antibiotics on the Gut Health of Zebrafish. Zebrafish. 2018; 15(4): 340–348. https://doi.org/10.1089/zeb.2017.1526Zhou L., Mchele Limbu S., Qiao F., Du Z.Y., Zhang M. Influence of Long-Term Feeding Antibiotics on the Gut Health of Zebrafish. Zebrafish. 2018; 15(4): 340–348. https://doi.org/10.1089/zeb.2017.1526Trushenski J.T. et al. Oxytetracycline does not cause growth promotion in finfish. Journal of Animal Science. 2018; 96(5): 1667–1677. https://doi.org/10.1093/jas/sky120Trushenski J.T. et al. Oxytetracycline does not cause growth promotion in finfish. Journal of Animal Science. 2018; 96(5): 1667–1677. https://doi.org/10.1093/jas/sky120Huang L. et al. Occurrence, distribution, and health risk assessment of quinolone antibiotics in water, sediment, and fish species of Qingshitan reservoir, South China. Scientific Reports. 2020; 10: 15777. https://doi.org/10.1038/s41598-020-72324-9Huang L. et al. Occurrence, distribution, and health risk assessment of quinolone antibiotics in water, sediment, and fish species of Qingshitan reservoir, South China. Scientific Reports. 2020; 10: 15777. https://doi.org/10.1038/s41598-020-72324-9Dawood M.A.O., Abo-Al-Ela H.G., Hasan M.T. Modulation of transcriptomic profile in aquatic animals: Probiotics, prebiotics and synbiotics scenarios. Fish & Shellfish Immunology. 2020; 97: 268–282. https://doi.org/10.1016/j.fsi.2019.12.054Dawood M.A.O., Abo-Al-Ela H.G., Hasan M.T. Modulation of transcriptomic profile in aquatic animals: Probiotics, prebiotics and synbiotics scenarios. Fish & Shellfish Immunology. 2020; 97: 268–282. https://doi.org/10.1016/j.fsi.2019.12.054Arsène M.M.J. et al. The use of probiotics in animal feeding for safe production and as potential alternatives to antibiotics. Veterinary World. 2021; 14(2): 319–328. https://doi.org/10.14202/vetworld.2021.319-328Arsène M.M.J. et al. The use of probiotics in animal feeding for safe production and as potential alternatives to antibiotics. Veterinary World. 2021; 14(2): 319–328. https://doi.org/10.14202/vetworld.2021.319-328Melara E.G., Avellaneda M.C., Valdivié M., García-Hernández Y., Aroche R., Martínez Y. Probiotics: Symbiotic Relationship with the Animal Host. Animals. 2022; 12: 719. https://doi.org/10.3390/ani12060719Melara E.G., Avellaneda M.C., Valdivié M., García-Hernández Y., Aroche R., Martínez Y. Probiotics: Symbiotic Relationship with the Animal Host. Animals. 2022; 12: 719. https://doi.org/10.3390/ani12060719Banerjee G., Ray A.K. The advancement of probiotics research and its application in fish farming industries. Research in Veterinary Science. 2017; 115: 66–77. https://doi.org/10.1016/j.rvsc.2017.01.016Banerjee G., Ray A.K. The advancement of probiotics research and its application in fish farming industries. Research in Veterinary Science. 2017; 115: 66–77. https://doi.org/10.1016/j.rvsc.2017.01.016Hai N.V. Research findings from the use of probiotics in tilapia aquaculture: A review. Fish & Shellfish Immunology. 2015; 45(2): 592–597. https://doi.org/10.1016/j.fsi.2015.05.026Hai N.V. Research findings from the use of probiotics in tilapia aquaculture: A review. Fish & Shellfish Immunology. 2015; 45(2): 592–597. https://doi.org/ 10.1016/j.fsi.2015.05.026Gatesoupe F.J. Updating the importance of lactic acid bacteria in fish farming: natural occurrence and probiotic treatments. Journal of Molecular Microbiology and Biotechnology. 2008; 14(1–3): 107–114. https://doi.org/10.1159/000106089Gatesoupe F.J. Updating the importance of lactic acid bacteria in fish farming: natural occurrence and probiotic treatments. Journal of Molecular Microbiology and Biotechnology. 2008; 14(1–3): 107–114. https://doi.org/10.1159/000106089Meidong R. et al. A novel probiotic Bacillus siamensis B44v isolated from Thai pickled vegetables (Phak-dong) for potential use as a feed supplement in aquaculture. The Journal of General and Applied Microbiology. 2017; 63(4): 246–253. https://doi.org/10.2323/jgam.2016.12.002Meidong R. et al. A novel probiotic Bacillus siamensis B44v isolated from Thai pickled vegetables (Phak-dong) for potential use as a feed supplement in aquaculture. The Journal of General and Applied Microbiology. 2017; 63(4): 246–253. https://doi.org/10.2323/jgam.2016.12.002Olmos J., Acosta M., Mendoza G., Pitones V. Bacillus subtilis, an ideal probiotic bacterium to shrimp and fish aquaculture that increase feed digestibility, prevent microbial diseases, and avoid water pollution. Archives of Microbiology. 2020; 202(3): 427–435. https://doi.org/10.1007/s00203-019-01757-2Olmos J., Acosta M., Mendoza G., Pitones V. Bacillus subtilis, an ideal probiotic bacterium to shrimp and fish aquaculture that increase feed digestibility, prevent microbial diseases, and avoid water pollution. Archives of Microbiology. 2020; 202(3): 427–435. https://doi.org/10.1007/s00203-019-01757-2Hai N.V. The use of probiotics in aquaculture. Journal of Applied Microbiology. 2015; 119(4): 917–935. https://doi.org/10.1111/jam.12886Hai N.V. The use of probiotics in aquaculture. Journal of Applied Microbiology. 2015; 119(4): 917–935. https://doi.org/10.1111/jam.12886Rashmeei M., Shekarabi S.P.H., Mehrgan M.S., Paknejad H. Stimulatory effect of dietary chasteberry (Vitex agnus-castus) extract on immunity, some immune-related gene expression, and resistance against Aeromonas hydrophila infection in goldfish (Carassius auratus). Fish & Shellfish Immunology. 2020; 107(A): 129–136. https://doi.org/10.1016/j.fsi.2020.09.037Rashmeei M., Shekarabi S.P.H., Mehrgan M.S., Paknejad H. Stimulatory effect of dietary chasteberry (Vitex agnus-castus) extract on immunity, some immune-related gene expression, and resistance against Aeromonas hydrophila infection in goldfish (Carassius auratus). Fish & Shellfish Immunology. 2020; 107(A): 129–136. https://doi.org/10.1016/j.fsi.2020.09.037Reverter M., Bontemps N., Lecchini D., Banaigs B., Sasal P. Use of plant extracts in fish aquaculture as an alternative to chemotherapy: Current status and future perspectives. Aquaculture. 2014; 433: 50–61. https://doi.org/10.1016/j.aquaculture.2014.05.048Reverter M., Bontemps N., Lecchini D., Banaigs B., Sasal P. Use of plant extracts in fish aquaculture as an alternative to chemotherapy: Current status and future perspectives. Aquaculture. 2014; 433: 50–61. https://doi.org/10.1016/j.aquaculture.2014.05.048Awad E., Awaad A. Role of medicinal plants on growth performance and immune status in fish. Fish & Shellfish Immunology. 2017; 67: 40–54. https://doi.org/10.1016/j.fsi.2017.05.034Awad E., Awaad A. Role of medicinal plants on growth performance and immune status in fish. Fish & Shellfish Immunology. 2017; 67: 40–54. https://doi.org/10.1016/j.fsi.2017.05.034Hoseinifar S.H. et al. Dietary supplementation of lemon verbena (Aloysia citrodora) improved immunity, immune-related genes expression and antioxidant enzymes in rainbow trout (Oncorrhyncus mykiss). Fish & Shellfish Immunology. 2020; 99: 379–385. https://doi.org/10.1016/j.fsi.2020.02.006Hoseinifar S.H. et al. Dietary supplementation of lemon verbena (Aloysia citrodora) improved immunity, immune-related genes expression and antioxidant enzymes in rainbow trout (Oncorrhyncus mykiss). Fish & Shellfish Immunology. 2020; 99: 379–385. https://doi.org/10.1016/j.fsi.2020.02.006Yousefi M., Farsani M.N., Ghafarifarsani H., Hoseinifar S.H., Doan H.V. The effects of dietary supplementation of mistletoe (Viscum album) extract on the growth performance, antioxidant, and innate, immune responses of rainbow trout (Oncorhynchus mykiss). Aquaculture. 2021; 536: 736385. https://doi.org/10.1016/j.aquaculture.2021.736385Yousefi M., Farsani M.N., Ghafarifarsani H., Hoseinifar S.H., Doan H.V. The effects of dietary supplementation of mistletoe (Viscum album) extract on the growth performance, antioxidant, and innate, immune responses of rainbow trout (Oncorhynchus mykiss). Aquaculture. 2021; 536: 736385. https://doi.org/10.1016/j.aquaculture.2021.736385Ebrahimi E., Haghjou M., Nematollahi A., Goudarzian F. Effects of rosemary essential oil on growth performance and hematological parameters of young great sturgeon (Huso huso). Aquaculture. 2020; 521: 734909. https://doi.org/10.1016/j.aquaculture.2019.734909Ebrahimi E., Haghjou M., Nematollahi A., Goudarzian F. Effects of rosemary essential oil on growth performance and hematological parameters of young great sturgeon (Huso huso). Aquaculture. 2020; 521: 734909. https://doi.org/10.1016/j.aquaculture.2019.734909David L.H., Pinho S.M., Agostinho F., Kimpara J.M., Keesman K.J., Garcia F. Emergy synthesis for aquaculture: A review on its constraints and potentials. Reviews in Aquaculture. 2021; 13(2): 1119–1138. https://doi.org/10.1111/raq.12519David L.H., Pinho S.M., Agostinho F., Kimpara J.M., Keesman K.J., Garcia F. Emergy synthesis for aquaculture: A review on its constraints and potentials. Reviews in Aquaculture. 2021; 13(2): 1119–1138. https://doi.org/10.1111/raq.12519Paray B.A., El-Basuini M.F., Alagawany M., Albeshr M.F., Farah M.A., Dawood M.A.O. Yucca schidigera usage for healthy aquatic animals: potential roles for sustainability. Animals. 2021; 11(1): 93. https://doi.org/10.3390/ani11010093.Paray B.A., El-Basuini M.F., Alagawany M., Albeshr M.F., Farah M.A., Dawood M.A.O. Yucca schidigera usage for healthy aquatic animals: potential roles for sustainability. Animals. 2021; 11(1): 93. https://doi.org/10.3390/ani11010093.Dawood M.A.O., Koshio S., Esteban M.Á. Beneficial roles of feed additives as immunostimulants in aquaculture: a review. Reviews in Aquaculture. 2018; 10(4): 950–974. https://doi.org/10.1111/raq.12209Dawood M.A.O., Koshio S., Esteban M.Á. Beneficial roles of feed additives as immunostimulants in aquaculture: a review. Reviews in Aquaculture. 2018; 10(4): 950–974. https://doi.org/10.1111/raq.12209Limbu S.M., Chen L.Q., Zhang M.L., Du Z.Y. A global analysis on the systemic effects of antibiotics in cultured fish and their potential human health risk: a review. Reviews in Aquaculture. 2021; 13(2): 1015–1059. https://doi.org/10.1111/raq.12511Limbu S.M., Chen L.Q., Zhang M.L., Du Z.Y. A global analysis on the systemic effects of antibiotics in cultured fish and their potential human health risk: a review. Reviews in Aquaculture. 2021; 13(2): 1015–1059. https://doi.org/10.1111/raq.12511Zhou M., Yu S., Hong B., Li J., Han H., Qie G. Antibiotics control in aquaculture requires more than antibiotic-free feeds: a tilapia farming case. Environmental Pollution. 2021; 268(B): 115854. https://doi.org/10.1016/j.envpol.2020.115854Zhou M., Yu S., Hong B., Li J., Han H., Qie G. Antibiotics control in aquaculture requires more than antibiotic-free feeds: a tilapia farming case. Environmental Pollution. 2021; 268(B): 115854. https://doi.org/10.1016/j.envpol.2020.115854Gupta N., Rani Kar S., Chakraborty A. A review on medicinal plants and immune status of fish. Egyptian Journal of Aquatic Biology and Fisheries. 2021; 25(2): 897–912. https://doi.org/10.21608/ejabf.2021.170062Gupta N., Rani Kar S., Chakraborty A. A review on medicinal plants and immune status of fish. Egyptian Journal of Aquatic Biology and Fisheries. 2021; 25(2): 897–912. https://doi.org/10.21608/ejabf.2021.170062Ashry A.M. et al. The impact of dietary curcumin on the growth performance, intestinal antibacterial capacity, and haemato-biochemical parameters of Gilthead seabream (Sparus aurata). Animals. 2021; 11(6): 1779. https://doi.org/10.3390/ani11061779Ashry A.M. et al. The impact of dietary curcumin on the growth performance, intestinal antibacterial capacity, and haemato-biochemical parameters of Gilthead seabream (Sparus aurata). Animals. 2021; 11(6): 1779. https://doi.org/10.3390/ani11061779Reverter M., Tapissier-Bontemps N., Sarter S., Sasal P., Caruso D. Moving towards more sustainable aquaculture practices: a meta-analysis on the potential of plant-enriched diets to improve fish growth, immunity and disease resistance. Reviews in Aquaculture. 2021; 13(1): 537–555. https://doi.org/10.1111/raq.12485Reverter M., Tapissier-Bontemps N., Sarter S., Sasal P., Caruso D. Moving towards more sustainable aquaculture practices: a meta-analysis on the potential of plant-enriched diets to improve fish growth, immunity and disease resistance. Reviews in Aquaculture. 2021; 13(1): 537–555. https://doi.org/10.1111/raq.12485Falzon C.C., Balabanova A. Phytotherapy: An introduction to herbal medicine. Primary Care: Clinics in Office Practice. 2017; 44(2): 217–227. https://doi.org/10.1016/j.pop.2017.02.001Falzon C.C., Balabanova A. Phytotherapy: An introduction to herbal medicine. Primary Care: Clinics in Office Practice. 2017; 44(2): 217–227. https://doi.org/10.1016/j.pop.2017.02.001Raman R.P. Applicability, feasibility and efficacy of phytotherapy in aquatic animal health management. American Journal of Plant Sciences. 2017; 8(2): 257–287. https://doi.org/10.4236/ajps.2017.82019Raman R.P. Applicability, feasibility and efficacy of phytotherapy in aquatic animal health management. American Journal of Plant Sciences. 2017; 8(2): 257–287. https://doi.org/10.4236/ajps.2017.82019Vaseeharan B., Thaya R. Medicinal plant derivatives as immunostimulants: An alternative to chemotherapeutics and antibiotics in aquaculture. Aquaculture International. 2014; 22(3): 1079–1091. https://doi.org/10.1007/s10499-013-9729-3Vaseeharan B., Thaya R. Medicinal plant derivatives as immunostimulants: An alternative to chemotherapeutics and antibiotics in aquaculture. Aquaculture International. 2014; 22(3): 1079–1091. https://doi.org/10.1007/s10499-013-9729-3Souza C.dе.F., Baldissera M.D., Baldisserotto B., Heinzmann B.M., MartosSitcha J.A., Mancera J.M. Essential oils as stressreducing agents for fish aquaculture: A review. Frontiers in Physiology. 2019; 10: 785. https://doi.org/10.3389/fphys.2019.00785Souza C.dе.F., Baldissera M.D., Baldisserotto B., Heinzmann B.M., MartosSitcha J.A., Mancera J.M. Essential oils as stressreducing agents for fish aquaculture: A review. Frontiers in Physiology. 2019; 10: 785. https://doi.org/10.3389/fphys.2019.00785Vercelli C. et al. In vitro and in vivo evaluation of a new phytotherapic blend to treat acute externa otitis in dogs. Journal of Veterinary Pharmacology and Therapeutics. 2021; 44(6): 910–918. https://doi.org/10.1111/jvp.13000Vercelli C. et al. In vitro and in vivo evaluation of a new phytotherapic blend to treat acute externa otitis in dogs. Journal of Veterinary Pharmacology and Therapeutics. 2021; 44(6): 910–918. https://doi.org/10.1111/jvp.13000Citarasu T. Herbal biomedicines: A new opportunity for aquaculture industry. Aquaculture International. 2010; 18(3): 403–414. https://doi.org/10.1007/s10499-009-9253-7Citarasu T. Herbal biomedicines: A new opportunity for aquaculture industry. Aquaculture International. 2010; 18(3): 403–414. https://doi.org/10.1007/s10499-009-9253-7Dawood M.A.O. et al. Exploring the Roles of Dietary Herbal Essential Oils in Aquaculture: A Review. Animals . 2022; 12(7): 823. https://doi.org/10.3390/ani12070823Dawood M.A.O. et al. Exploring the Roles of Dietary Herbal Essential Oils in Aquaculture: A Review. Animals . 2022; 12(7): 823. https://doi.org/10.3390/ani12070823Bertocci F., Mannino G. Can Agri-Food Waste Be a Sustainable Alternative in Aquaculture? A Bibliometric and Meta-Analytic Study on Growth Performance, Innate Immune System, and Antioxidant Defenses. Foods. 2022; 11(13): 1861. https://doi.org/10.3390/foods11131861Bertocci F., Mannino G. Can Agri-Food Waste Be a Sustainable Alternative in Aquaculture? A Bibliometric and Meta-Analytic Study on Growth Performance, Innate Immune System, and Antioxidant Defenses. Foods. 2022; 11(13): 1861. https://doi.org/10.3390/foods11131861Ashry A.M. et al. Dietary anise (Pimpinella anisum L.) enhances growth performance and serum immunity of European sea bass (Dicentrarchus labrax). Aquaculture Reports. 2022; 23: 101083. https://doi.org/10.1016/j.aqrep.2022.101083Ashry A.M. et al. Dietary anise (Pimpinella anisum L.) enhances growth performance and serum immunity of European sea bass (Dicentrarchus labrax). Aquaculture Reports. 2022; 23: 101083. https://doi.org/10.1016/j.aqrep.2022.101083Ghafarifarsani H., Hoseinifar S.H., Adorian T.J., Ferrigolo F.R.G., Raissy M., Doan H.V. The effects of combined inclusion of Malvae sylvestris, Origanum vulgare, and Allium hirtifolium boiss for common carp (Cyprinus carpio) diet: Growth performance, antioxidant defense, and immunological parameters. Fish & Shellfish Immunology. 2021; 119: 670–677. https://doi.org/10.1016/j.fsi.2021.10.014Ghafarifarsani H., Hoseinifar S.H., Adorian T.J., Ferrigolo F.R.G., Raissy M., Doan H.V. The effects of combined inclusion of Malvae sylvestris, Origanum vulgare, and Allium hirtifolium boiss for common carp (Cyprinus carpio) diet: Growth performance, antioxidant defense, and immunological parameters. Fish & Shellfish Immunology. 2021; 119: 670–677. https://doi.org/10.1016/j.fsi.2021.10.014Pezhman S., Shekarabia H., Javarsiania L., Mehrgana M.S., Dawood M.A.O., Adelc M. Growth performance, blood biochemistry profile, and immune response of rainbow trout (Oncorhynchus mykiss) fed dietary Persian shallot (Allium stipitatum) powder. Aquaculture. 2022; 548(1): 737627. https://doi.org/10.1016/j.aquaculture.2021.737627Pezhman S., Shekarabia H., Javarsiania L., Mehrgana M.S., Dawood M.A.O., Adelc M. Growth performance, blood biochemistry profile, and immune response of rainbow trout (Oncorhynchus mykiss) fed dietary Persian shallot (Allium stipitatum) powder. Aquaculture. 2022; 548(1): 737627. https://doi.org/10.1016/j.aquaculture.2021.737627Shekarabi S.P.H. et al. Effect of dietary barberry fruit (Berberis vulgaris) extract on immune function, antioxidant capacity, antibacterial activity, and stress-related gene expression of Siberian sturgeon (Acipenser baerii). Aquaculture Reports. 2022; 23: 101041. https://doi.org/10.1016/j.aqrep.2022.101041Shekarabi S.P.H. et al. Effect of dietary barberry fruit (Berberis vulgaris) extract on immune function, antioxidant capacity, antibacterial activity, and stress-related gene expression of Siberian sturgeon (Acipenser baerii). Aquaculture Reports. 2022; 23: 101041. https://doi.org/10.1016/j.aqrep.2022.101041Wangkahart E., Wachiraamonloed S., Lee P.-T., Subramani P.A., Qi Z., Wang B. Impacts of Aegle marmelos fruit extract as a medicinal herb on growth performance, antioxidant and immune responses, digestive enzymes, and disease resistance against Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus). Fish & Shellfish Immunology. 2022; 120: 402–410. https://doi.org/10.1016/j.fsi.2021.11.015Wangkahart E., Wachiraamonloed S., Lee P.-T., Subramani P.A., Qi Z., Wang B. Impacts of Aegle marmelos fruit extract as a medicinal herb on growth performance, antioxidant and immune responses, digestive enzymes, and disease resistance against Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus). Fish & Shellfish Immunology. 2022; 120: 402–410. https://doi.org/10.1016/j.fsi.2021.11.015Ahmadifar E. et al. Cornelian cherry (Cornus mas L.) fruit extract improves growth performance, disease resistance, and serum immune-and antioxidantrelated gene expression of common carp (Cyprinus carpio). Aquaculture. 2022; 558: 738372. https://doi.org/10.1016/j.aquaculture.2022.738372Ahmadifar E. et al. Cornelian cherry (Cornus mas L.) fruit extract improves growth performance, disease resistance, and serum immune-and antioxidantrelated gene expression of common carp (Cyprinus carpio). Aquaculture. 2022; 558: 738372. https://doi.org/10.1016/j.aquaculture.2022.738372Abdel-Razek N., Awad S.M., Abdel-Tawwab M. Effect of dietary purslane (Portulaca oleracea L.) leaves powder on growth, immunostimulation, and protection of Nile tilapia, Oreochromis niloticus against Aeromonas hydrophila infection. Fish Physiology and Biochemistry. 2019; 45(6): 1907–1917. https://doi.org/10.1007/s10695-019-00685-8Abdel-Razek N., Awad S.M., Abdel-Tawwab M. Effect of dietary purslane (Portulaca oleracea L.) leaves powder on growth, immunostimulation, and protection of Nile tilapia, Oreochromis niloticus against Aeromonas hydrophila infection. Fish Physiology and Biochemistry. 2019; 45(6): 1907–1917. https://doi.org/10.1007/s10695-019-00685-8Abdel-Tawwab M., Sameer F., El-Nabi A.S.A., Monnier M.N. Antioxidative and immunostimulatory effect of dietary cinnamon nanoparticles on the performance of Nile tilapia, Oreochromis niloticus (L.) and its susceptibility to hypoxia stress and Aeromonas hydrophila infection. Fish & Shellfish Immunology. 2018; 74: 19–25. https://doi.org/10.1016/j.fsi.2017.12.033Abdel-Tawwab M., Sameer F., El-Nabi A.S.A., Monnier M.N. Antioxidative and immunostimulatory effect of dietary cinnamon nanoparticles on the performance of Nile tilapia, Oreochromis niloticus (L.) and its susceptibility to hypoxia stress and Aeromonas hydrophila infection. Fish & Shellfish Immunology. 2018; 74: 19–25. https://doi.org/10.1016/j.fsi.2017.12.033Abdel-Tawwab M., Adeshina I., Jenyo-Oni A., Ajani E.K., Emikpe B.O. Growth, physiological, antioxidants, and immune response of African catfish, Clarias gariepinus (B.), to dietary clove basil, Ocimum gratissimum, leaf extract and its susceptibility to Listeria monocytogenes infection. Fish & Shellfish Immunology. 2018; 78: 346–354. https://doi.org/10.1016/j.fsi.2018.04.057Abdel-Tawwab M., Adeshina I., Jenyo-Oni A., Ajani E.K., Emikpe B.O. Growth, physiological, antioxidants, and immune response of African catfish, Clarias gariepinus (B.), to dietary clove basil, Ocimum gratissimum, leaf extract and its susceptibility to Listeria monocytogenes infection. Fish & Shellfish Immunology. 2018; 78: 346–354. https://doi.org/10.1016/j.fsi.2018.04.057Mahboub H.H. et al. Immune-antioxidant trait, Aeromonas veronii resistance, growth, intestinal architecture, and splenic cytokines expression of Cyprinus carpio fed Prunus armeniaca kernel-enriched diets. Fish & Shellfish Immunology. 2022; 124: 182–191. https://doi.org/10.1016/j.fsi.2022.03.048Mahboub H.H. et al. Immune-antioxidant trait, Aeromonas veronii resistance, growth, intestinal architecture, and splenic cytokines expression of Cyprinus carpio fed Prunus armeniaca kernel-enriched diets. Fish & Shellfish Immunology. 2022; 124: 182–191. https://doi.org/10.1016/j.fsi.2022.03.048Rashmeei M., Shekarabi S.P.H., Mehrgan M.S., Paknejad H. Assessment of dietary chaste tree (Vitex agnus-castus) fruit extract on growth performance, hemato-biochemical parameters, and mRNA levels of growth and appetiterelated genes in goldfish (Carassius auratus). Aquaculture and Fisheries. 2022; 7(3): 296–303. https://doi.org/10.1016/j.aaf.2021.01.007Rashmeei M., Shekarabi S.P.H., Mehrgan M.S., Paknejad H. Assessment of dietary chaste tree (Vitex agnus-castus) fruit extract on growth performance, hemato-biochemical parameters, and mRNA levels of growth and appetiterelated genes in goldfish (Carassius auratus). Aquaculture and Fisheries. 2022; 7(3): 296–303. https://doi.org/10.1016/j.aaf.2021.01.007Raissy M., Ghafarifarsani H., Hoseinifar S.H., El-Haroun E.R., Shahbazi N.S., Doan H.V. The effect of dietary combined herbs extracts (oak acorn, coriander, and common mallow) on growth, digestive enzymes, antioxidant and immune response, and resistance against Aeromonas hydrophila infection in common carp, Cyprinus carpio. Aquaculture. 2022; 546: 737287. https://doi.org/10.1016/j.aquaculture.2021.737287Raissy M., Ghafarifarsani H., Hoseinifar S.H., El-Haroun E.R., Shahbazi N.S., Doan H.V. The effect of dietary combined herbs extracts (oak acorn, coriander, and common mallow) on growth, digestive enzymes, antioxidant and immune response, and resistance against Aeromonas hydrophila infection in common carp, Cyprinus carpio. Aquaculture. 2022; 546: 737287. https://doi.org/10.1016/j.aquaculture.2021.737287Rudiansyah M. et al. Beneficial alterations in growth performance, blood biochemicals, immune responses, and antioxidant capacity of common carp (Cyprinus carpio) fed a blend of Thymus vulgaris, Origanum majorana, and Satureja hortensis extracts. Aquaculture. 2022; 555: 738254. https://doi.org/10.1016/j.aquaculture.2022.738254Rudiansyah M. et al. Beneficial alterations in growth performance, blood biochemicals, immune responses, and antioxidant capacity of common carp (Cyprinus carpio) fed a blend of Thymus vulgaris, Origanum majorana, and Satureja hortensis extracts. Aquaculture. 2022; 555: 738254. https://doi.org/10.1016/j.aquaculture.2022.738254Cheng C., Park S.C., Giri S.S. Effect of Pandanus tectorius extract as food additive on oxidative stress, immune status, and disease resistance in Cyprinus carpio. Fish & Shellfish Immunology. 2022; 120: 287–294. https://doi.org/10.1016/j.fsi.2021.12.004Cheng C., Park S.C., Giri S.S. Effect of Pandanus tectorius extract as food additive on oxidative stress, immune status, and disease resistance in Cyprinus carpio. Fish & Shellfish Immunology. 2022; 120: 287–294. https://doi.org/10.1016/j.fsi.2021.12.004Abdul Kari Z. et al. Role of phytobiotics in relieving the impacts of Aeromonas hydrophila infection on aquatic animals: A mini-review. Front Vet Sci. 2022; 9: 1023784. https://doi.org/10.3389/fvets.2022.1023784.Abdul Kari Z. et al. Role of phytobiotics in relieving the impacts of Aeromonas hydrophila infection on aquatic animals: A mini-review. Front Vet Sci. 2022; 9: 1023784. https://doi.org/10.3389/fvets.2022.1023784.Pachanawan A., Phumkhachorn P., Rattanachaikunsopon P. Potential of Psidium guajava supplemented fish diets in controlling Aeromonas hydrophila infection in tilapia (Oreochromis niloticus). J. Biosci Bioeng. 2008; 106(5): 419–424. https://doi.org/10.1263/jbb.106.419.Pachanawan A., Phumkhachorn P., Rattanachaikunsopon P. Potential of Psidium guajava supplemented fish diets in controlling Aeromonas hydrophila infection in tilapia (Oreochromis niloticus). J. Biosci Bioeng. 2008; 106(5): 419–424. https://doi.org/10.1263/jbb.106.419.Ardó L., Yin G., Xu P., Váradi L., Szigeti G., Jeney Z. et al. Chinese herbs (Astragalus membranaceus and Lonicera japonica) and boron enhance the nonspecific immune response of Nile tilapia (Oreochromis niloticus) and resistance against Aeromonas hydrophila. Aquaculture. 2008; 275: 26–33. https://doi.org/10.1016/j.aquaculture.2007.12.022Ardó L., Yin G., Xu P., Váradi L., Szigeti G., Jeney Z. et al. Chinese herbs (Astragalus membranaceus and Lonicera japonica) and boron enhance the nonspecific immune response of Nile tilapia (Oreochromis niloticus) and resistance against Aeromonas hydrophila. Aquaculture. 2008; 275: 26–33. https://doi.org/10.1016/j.aquaculture.2007.12.022Abdel-Tawwab M. et al. Use of green tea, Camellia sinensis L., in practical diet for growth and protection of Nile tilapia, Oreochromis niloticus (L.), against Aeromonas hydrophila infection. Journal of the World Aquaculture Society 2010; 41: 203–213. https://doi.org/10.1111/j.1749-7345.2010.00360.x.Abdel-Tawwab M. et al. Use of green tea, Camellia sinensis L., in practical diet for growth and protection of Nile tilapia, Oreochromis niloticus (L.), against Aeromonas hydrophila infection. Journal of the World Aquaculture Society 2010; 41: 203–213. https://doi.org/10.1111/j.1749-7345.2010.00360.x.Ahmad M.H., El Mesallamy A.M., Samir F., Zahran F. Effect of cinnamon (Cinnamomum zeylanicum) on growth performance, feed utilization, whole-body composition, and resistance to Aeromonas hydrophila in Nile tilapia. J. Appl Aquacult. 2011; 23: 289–298. https://doi.org/10.1080/10454438.2011.626350Ahmad M.H., El Mesallamy A.M., Samir F., Zahran F. Effect of cinnamon (Cinnamomum zeylanicum) on growth performance, feed utilization, whole-body composition, and resistance to Aeromonas hydrophila in Nile tilapia. J. Appl Aquacult. 2011; 23: 289–298. https://doi.org/10.1080/10454438.2011.626350Abdel-Tawwab M. The use of American ginseng (Panax quinquefolium) in practical diets for Nile tilapia (Oreochromis niloticus): growth performance and challenge with Aeromonas hydrophila. Journal of Applied Aquaculture. 2012; 24(4) : 366-376. https://doi.org/10.1080/10454438.2012.733593Abdel-Tawwab M. The use of American ginseng (Panax quinquefolium) in practical diets for Nile tilapia (Oreochromis niloticus): growth performance and challenge with Aeromonas hydrophila. Journal of Applied Aquaculture. 2012; 24(4) : 366-376. https://doi.org/10.1080/10454438.2012.733593Park K.H., Choi S.H. The effect of mistletoe, Viscum album coloratum, extract on innate immune response of Nile tilapia (Oreochromis niloticus). Fish Shellfish Immunol. 2012; 32(6): 1016–1021. https://doi.org/10.1016/j.fsi.2012.02.023.Park K.H., Choi S.H. The effect of mistletoe, Viscum album coloratum, extract on innate immune response of Nile tilapia (Oreochromis niloticus). Fish Shellfish Immunol. 2012; 32(6): 1016–1021. https://doi.org/10.1016/j.fsi.2012.02.023.Mohammadi G., Rafiee G., El Basuini M.F., Abdel-Latif H.M.R., Dawood M.A.O. The growth performance, antioxidant capacity, immunological responses, and the resistance against Aeromonas hydrophila in Nile tilapia (Oreochromis niloticus) fed Pistacia vera hulls derived polysaccharide. Fish Shellfish Immunol. 2020; 106: 36–43. https://doi.org/10.1016/j.fsi.2020.07.064.Mohammadi G., Rafiee G., El Basuini M.F., Abdel-Latif H.M.R., Dawood M.A.O. The growth performance, antioxidant capacity, immunological responses, and the resistance against Aeromonas hydrophila in Nile tilapia (Oreochromis niloticus) fed Pistacia vera hulls derived polysaccharide. Fish Shellfish Immunol. 2020; 106: 36–43. https://doi.org/10.1016/j.fsi.2020.07.064.Naiel M.A., Ismael N.E., Negm S.S., Ayyat M.S., Al-Sagheer A.A. Rosemary leaf powder-supplemented diet enhances performance, antioxidant properties, immune status, and resistance against bacterial diseases in Nile Tilapia (Oreochromis niloticus). Aquaculture. 2020; 526: 735370. https://doi.org/10.1016/j.aquaculture.2020.735370Naiel M.A., Ismael N.E., Negm S.S., Ayyat M.S., Al-Sagheer A.A. Rosemary leaf powder-supplemented diet enhances performance, antioxidant properties, immune status, and resistance against bacterial diseases in Nile Tilapia (Oreochromis niloticus). Aquaculture. 2020; 526: 735370. https://doi.org/10.1016/j.aquaculture.2020.735370Estaiano de Rezende R.A. et al. Phytobiotics blend as a dietary supplement for Nile tilapia health improvement. Fish Shellfish Immunol. 2021; 114: 293–300. https://doi.org/10.1016/j.fsi.2021.05.010.Estaiano de Rezende R.A. et al. Phytobiotics blend as a dietary supplement for Nile tilapia health improvement. Fish Shellfish Immunol. 2021; 114: 293–300. https://doi.org/10.1016/j.fsi.2021.05.010.Abd El-latif A.M., Abd El-Gawad E.A., Soror E.I., Shourbela R.M., Zahran E. Dietary supplementation with miswak (Salvadora persica) improves the health status of Nile tilapia and protects against Aeromonas hydrophila infection. Aquacult Rep. 2021; 19: 100594. https://doi.org/10.1016/j.aqrep.2021.100594Abd El-latif A.M., Abd El-Gawad E.A., Soror E.I., Shourbela R.M., Zahran E. Dietary supplementation with miswak (Salvadora persica) improves the health status of Nile tilapia and protects against Aeromonas hydrophila infection. Aquacult Rep. 2021; 19: 100594. https://doi.org/10.1016/j.aqrep.2021.100594da Silva Gündel S. et al. Nanoemulsions containing Cymbopogon flexuosus essential oil: Development, characterization, stability study and evaluation of antimicrobial and antibiofilm activities Microbial Pathogenesis. 2018; 118: 268–276. https://doi.org/10.1016/j.micpath.2018.03.043da Silva Gündel S. et al. Nanoemulsions containing Cymbopogon flexuosus essential oil: Development, characterization, stability study and evaluation of antimicrobial and antibiofilm activities Microbial Pathogenesis. 2018; 118: 268–276. https://doi.org/10.1016/j.micpath.2018.03.043Gao S. et al. Antimicrobial Activity of Lemongrass Essential Oil (Cymbopogon flexuosus) and Its Active Component Citral Against Dual-Species Biofilms of Staphylococcus aureus and Candida Species. Frontiers in Cellular and Infection Microbiology. 2020; 10: 603858. https://doi.org/10.3389/fcimb.2020.603858Gao S. et al. Antimicrobial Activity of Lemongrass Essential Oil (Cymbopogon flexuosus) and Its Active Component Citral Against Dual-Species Biofilms of Staphylococcus aureus and Candida Species. Frontiers in Cellular and Infection Microbiology. 2020; 10: 603858. https://doi.org/10.3389/fcimb.2020.603858Rossi G.G. et al. Antibiofilm activity of nanoemulsions of Cymbopogon flexuosus against rapidly growing mycobacteria. Microbial Pathogenesis. 2017; 113: 335–341. https://doi.org/10.1016/j.micpath.2017.11.002Rossi G.G. et al. Antibiofilm activity of nanoemulsions of Cymbopogon flexuosus against rapidly growing mycobacteria. Microbial Pathogenesis. 2017; 113: 335–341. https://doi.org/10.1016/j.micpath.2017.11.002De Souza E.Â.M. et al. Cymbopogon flexuosus essential oil as an additive improves growth, biochemical and physiological responses and survival against Aeromonas hydrophila infection in Nile tilapia. Annals of the Brazilian Academy of Sciences. 2020; 92(1): e20190140. https://doi.org/10.1590/0001-3765202020190140De Souza E.Â.M. et al. Cymbopogon flexuosus essential oil as an additive improves growth, biochemical and physiological responses and survival against Aeromonas hydrophila infection in Nile tilapia. Annals of the Brazilian Academy of Sciences. 2020; 92(1): e20190140. https://doi.org/10.1590/0001-3765202020190140

The authors declare that there are no conflicts of interest present.