vetpressАграрная наукаAgrarian science0869-81552686-701XРедакция журнала "Аграрная наука"10.32634/0869-8155-2023-373-8-27-35vetpress-2743Research ArticleЗООТЕХНИЯ И ВЕТЕРИНАРИЯZOOTECHNICS AND VETERINARY MEDICINEЦирковирус как фактор, контролирующий эффективность беременности у свиноматокCircovirus as a factor controlling the effectiveness of pregnancy in sowshttps://orcid.org/0000-0001-7515-5670БурковП. В.BurkovP. V.

Павел Валерьевич Бурков, кандидат ветеринарных наук, руководитель научно-исследовательского  центра биотехнологии репродукции животных

ул. Гагарина, 13, Троицк, Челябинская обл., 457100, Россия

Pavel Valerievich Burkov, Candidate of Veterinary Sciences, Head of the Research Center for Animal Reproduction Biotechnology

13 Gagarin Str., Troitsk, Chelyabinsk region, 457100, Russia 

burcovpavel@mail.ruhttps://orcid.org/0000-0003-3818-0556ДерхоМ. А.DerkhoM. A.

Марина Аркадьевна Дерхо, доктор биологических наук, профессор

ул. Гагарина, 13, Троицк, Челябинская обл., 457100, Россия 

Marina Arkadyevna Derkho, Doctor of Biological Sciences, Professor, South Ural State Agrarian University 

13 Gagarin Str., Troitsk, Chelyabinsk region, 457100, Russia 

derkho2010@yandex.ruhttps://orcid.org/0000-0003-0857-5143РебезовМ. Б.RebezovM. B.

Максим Борисович Ребезов, доктор  сельскохозяйственных наук, профессор, главный научный сотрудник; доктор сельскохозяйственных наук, профессор кафедры биотехнологии и пищевых продуктов

ул. Талалихина, 26, Москва, 109316, Россияул. Карла Либкнехта, 42, Екатеринбург, 620075, Россия

Maksim Borisovich Rebezov, Doctor of Agricultural Sciences, Professor, Chief Researcher; Doctor of Agricultural Sciences, Professor of the Departmentof Biotechnology and Food Products

26 Talalikhin Str., Moscow, 109316, Russian Federation

42 Karl Liebknecht Str., Yekaterinburg, 620075, Russia 

rebezov@ya.ruhttps://orcid.org/0000-0001-8685-4645ЩербаковП. Н.ScherbakovP. N.

Павел Николаевич Щербаков, доктор ветеринарных наук, профессор кафедры инфекционных болезней и ветеринарно-санитарной экспертизы

ул. Гагарина, 13, Троицк, Челябинская обл., 457100, Россия 

Pavel Nikolaevich Shcherbakov, Doctor of Veterinary Sciences, Professor of the Department of Infectious Diseases and Veterinary and Sanitary Expertise 

13 Gagarin Str., Troitsk, Chelyabinsk region, 457100, Russia 

scherbakov_pavel@mail.ruЮжно-Уральский государственный аграрный университетРоссияSouth Ural State Agrarian UniversityRussian FederationФедеральный научный центр пищевых систем им. В.М. Горбатова Российской академии наук; Уральский государственный аграрный университетРоссияV.M. Gorbatov Federal Research Center for Food Systems of the Russian Academy of Sciences; Ural State Agrarian UniversityRussian Federation202325082023182735Copyright © Бурков П.В., Дерхо М.А., Ребезов М.Б., Щербаков П.Н., 20232023Бурков П.В., Дерхо М.А., Ребезов М.Б., Щербаков П.Н.Burkov P.V., Derkho M.A., Rebezov M.B., Scherbakov P.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vetpress.ru/jour/article/view/2743

Приведены результаты микроскопического исследования внутренних органов вирусно абортированных плодов с целью выяснения причин репродуктивных потерь в условиях субклинически протекающей цирковирусной инфекции у свиноматок. Материалом исследования служили ткани печени, плаценты, пуповины, селезенки и головного мозга аборт-плодов свиноматок, беременность которых прервалась в последний триместр супоросности Они имели клинические признаки заболеваний, связанных с инфекцией ЦВС-2. Установлено, что вирус ЦВС-2 обладает способностью проникать через фетоплацентарный барьер из организма матери. За счет инфицирования пуповины и плаценты он поступает в плод, в котором проявляет тропность по отношению к клеткам печени, селезенки и головного мозга. Развитие вируса в клетках данных органов является причиной развития в них воспалительных, дистрофических и некротических процессов, влияя на процессы их внутриутробного развития, поэтому в последний триместр беременности клетки печени, селезенки и головного мозгане обладают функциональными свойствами, соответствующими сроку беременности, что сказывается на их жизнеспособности.Исследование демонстрирует роль цирковирусной инфекции в формировании репродуктивных потерь у свиноматок в промышленных условиях.

The results of a microscopic examination of the internal organs of virally aborted fetuses are presented in order to determine the causes of reproductive losses in conditions of subclinical circovirus infection in sows. The material of the study was the tissues of the liver, placenta, umbilical cord, spleen and brain of abortion fetuses of sows whose pregnancy was interrupted in the last trimester of pregnancy. They had clinical signs of diseases associated with PCV-2 infection. It has been established that the PCV-2 virus has the ability to penetrate the feto-placental barrier from the mother's body; due to infection of the umbilical cord and placenta, it enters the fetus, in which it exhibits tropism in relation to the cells of the liver, spleen and brain. The development of the virus in the cells of these organs is the cause of the development of inflammatory, dystrophic and necrotic processes in them, affecting the processes of their intrauterine development, therefore in the last trimester of pregnancy, the cells of the liver, spleen and brain do not have functional properties corresponding to the duration of pregnancy, which affects their viability. The study demonstrates the role of circovirus infection in the formation of reproductive losses in sows in industrial conditions.

цирковируссвиноматкибеременностьаборт-плодымикроскопические исследованияcircovirussowspregnancylabortion fetusesmicroscopic studiesДанное исследование финансируется в рамках регионального конкурса Российского научного фонда 2021 года «Проведение фундаментальных научных исследований и поисковых научных исследований отдельными научными группами» (соглашение от 25.03.2022 № 22-16-20007).This research is funded within the framework of the regional competition of the Russian Science Foundation in 2021 «Conducting fundamental scientific research and exploratory scientific research by individual scientific groups» (agreement No. 22-16-20007 of 03.25.2022).ReferencesBurkov P.V. et al. Pathological features of the lungs and liver of piglets under conditions of constant vaccination of livestock against circovirus infection. Теория и практика переработки мяса. 2023; 8(1): 4–11. https://doi.org/10.21323/2414-438X-2023-8-1-4-11Burkov P.V. et al. Pathological features of the lungs and liver of piglets underMeng X.-J. Porcine Circovirus Type 2 (PCV2): Pathogenesis and Interaction with the Immune System. Annual Review of Animal Biosciences. 2013; 1: 43–64. https://doi.org/10.1146/annurev-animal-031412-103720conditions of constant vaccination of livestock against circovirus infection.Бурков П.В., Щербаков П.Н., Дерхо М.А., Ребезов М.Б. Особенности формирования поствакцинального иммунитета против цирковирусной инфекции свиней и его коррекции. Аграрная наука. 2022; (10): 32–37. https://doi.org/10.32634/0869-8155-2022-363-10-32-37Theory and practice of meat processing. 2023; 8(1): 4–11. https://doi.org/10.21323/2414-438X-2023-8-1-4-11Gauger P.C. et al. Leukogram abnormalities in gnotobiotic pigs infected with porcine circovirus type 2. Veterinary Microbiology. 2011; 154(1–2): 185–190. https://doi.org/10.1016/j.vetmic.2011.06.016Meng X.-J. Porcine Circovirus Type 2 (PCV2): Pathogenesis and Interaction with the Immune System. Annual Review of Animal Biosciences. 2013; 1: 43–64. https://doi.org/10.1146/annurev-animal-031412-103720Boulbria G. et al. Haematological reference intervals of sows at end gestation in ten French herds, the impact of parity on haematological parameters and the consequences on reproductive performance. Porcine Health Management. 2021; 7: 47. https://doi.org/10.1186/s40813-021-00227-wBurkov P.V., Scherbakov P.N., Derkho M.A., Rebezov M.B. Aspects of the formation of post-vaccination immunity against porcine circovirus infection and its correction. Agrarian science. 2022; (10): 32–37 (In Russian). https://doi.org/10.32634/0869-8155-2022-363-10-32-37Yang K.Y., Jeon J.H., Kwon K.S., Choi H.C., Kim J.B., Lee J.Y. Effect of different parities on reproductive performance, birth intervals, and tail behavior in sows. Journal of Animal Science and Technology. 2019; 61(3): 147–153. https://doi.org/10.5187/jast.2019.61.3.147Gauger P.C. et al. Leukogram abnormalities in gnotobiotic pigs infected with porcine circovirus type 2. Veterinary Microbiology. 2011; 154(1–2): 185–190. https://doi.org/10.1016/j.vetmic.2011.06.016Koketsu Y., Iida R. Farm data analysis for lifetime performance components of sows and their predictors in breeding herds. Porcine Health Management. 2020; 6: 24. https://doi.org/10.1186/s40813-020-00163-1Boulbria G. et al. Haematological reference intervals of sows at end gestation in ten French herds, the impact of parity on haematological parameters and the consequences on reproductive performance. Porcine Health Management. 2021; 7: 47. https://doi.org/10.1186/s40813-021-00227-wJežek J. et al. The influence of age, farm, and physiological status on pig hematological profiles. Journal of Swine Health and Production. 2018; 26(2): 72–78.Yang K.Y., Jeon J.H., Kwon K.S., Choi H.C., Kim J.B., Lee J.Y. Effect of different parities on reproductive performance, birth intervals, and tail behavior in sows. Journal of Animal Science and Technology. 2019; 61(3): 147–153. https://doi.org/10.5187/jast.2019.61.3.147Brissonnier M. et al. Frequency of infection with Mycoplasma suis in gestating sows using qPCR on ten commercial French herds, and impact of the infection on clinical, haematological and biochemical parameters. Porcine Health Management. 2020; 6: 13. https://doi.org/10.1186/s40813-020-00152-4Koketsu Y., Iida R. Farm data analysis for lifetime performance components of sows and their predictors in breeding herds. Porcine Health Management. 2020; 6: 24. https://doi.org/10.1186/s40813-020-00163-1Stukelj M., Toplak I., Nemec Svete A. Blood antioxidant enzymes (SOD, GPX), biochemical and haematological parameters in pigs naturally infected with porcine reproductive and respiratory syndrome virus. Polish Journal of Veterinary Sciences. 2013; 16(2): 369–376. https://doi.org/10.2478/pjvs-2013-0049Ježek J. et al. The influence of age, farm, and physiological status on pig hematological profiles. Journal of Swine Health and Production. 2018; 26(2): 72–78.Ladinig A., Gerner W., Saalmüller A., Lunney J.K., Ashley C., Harding J.C.S. Changes in leukocyte subsets of pregnant gilts experimentally infected with porcine reproductive and respiratory syndrome virus and relationships with viral load and fetal outcome. Veterinary Research. 2014; 45: 128. https://doi.org/10.1186/s13567-014-0128-1Brissonnier M. et al. Frequency of infection with Mycoplasma suis in gestating sows using qPCR on ten commercial French herds, and impact of the infection on clinical, haematological and biochemical parameters. Porcine Health Management. 2020; 6: 13. https://doi.org/10.1186/s40813-020-00152-4Saporiti V. et al. Porcine Circovirus 3 Detection in Aborted Fetuses and Stillborn Piglets from Swine Reproductive Failure Cases. Viruses. 2022; 13(2): 264. https://doi.org/10.3390/v13020264Stukelj M., Toplak I., Nemec Svete A. Blood antioxidant enzymes (SOD, GPX), biochemical and haematological parameters in pigs naturally infected with porcine reproductive and respiratory syndrome virus. Polish Journal of Veterinary Sciences. 2013; 16(2): 369–376. https://doi.org/10.2478/pjvs-2013-0049Nielsen J. et al. Association of lymphopenia with porcine circovirus type 2 induced postweaning multisystemic wasting syndrome (PMWS). Veterinary Immunology and Immunopathology. 2003; 92(3–4): 7–111. https://doi.org/10.1016/s0165-2427(03)00031-xLadinig A., Gerner W., Saalmüller A., Lunney J.K., Ashley C., Harding J.C.S. Changes in leukocyte subsets of pregnant gilts experimentally infected with porcine reproductive and respiratory syndrome virus and relationships with viral load and fetal outcome. Veterinary Research. 2014; 45: 128. https://doi.org/10.1186/s13567-014-0128-1Brunborg I.M. et al. Association of Myocarditis with High Viral Load of Porcine Circovirus Type 2 in Several Tissues in Cases of Fetal Death and High Mortality in Piglets. A Case Study. Journal of Veterinary Diagnostic Investigation. 2007; 19(4): 368–375. https://doi.org/10.1177/104063870701900405Saporiti V. et al. Porcine Circovirus 3 Detection in Aborted Fetuses and Stillborn Piglets from Swine Reproductive Failure Cases. Viruses. 2022; 13(2): 264. https://doi.org/10.3390/v13020264Madson D.M., Patterson A.R., Ramamoorthy S., Pal N., Meng X.J., Opriessnig T. Effect of Porcine Circovirus Type 2 (PCV2) Vaccination of the Dam on PCV2 Replication In Utero. Clinical and Vaccine Immunology. 2009; 16(6): 830–834. https://doi.org/10.1128/CVI.00455-08Nielsen J. et al. Association of lymphopenia with porcine circovirus type 2 induced postweaning multisystemic wasting syndrome (PMWS). Veterinary Immunology and Immunopathology. 2003; 92(3–4): 7–111. https://doi.org/10.1016/s0165-2427(03)00031-xDal Santo A.C., Cezario K.C., Bennemann P.E., Machado S.A., Martins M. Full-genome sequences of porcine circovirus 3 (PCV3) and high prevalence in mummified fetuses from commercial farms in Brazil. Microbial Pathogenesis. 2020; 141: 104027. https://doi.org/10.1016/j.micpath.2020.104027Brunborg I.M. et al. Association of Myocarditis with High Viral Load of Porcine Circovirus Type 2 in Several Tissues in Cases of Fetal Death and High Mortality in Piglets. A Case Study. Journal of Veterinary Diagnostic Investigation. 2007; 19(4): 368–375. https://doi.org/10.1177/104063870701900405Tummaruk P., Pearodwong P. Expression of PCV2 antigen in the ovarian tissues of gilts. Journal of Veterinary Medical Science. 2016; 78(3): 457–461. https://doi.org/10.1292/jvms.15-0450Madson D.M., Patterson A.R., Ramamoorthy S., Pal N., Meng X.J., Opriessnig T. Effect of Porcine Circovirus Type 2 (PCV2) Vaccination of the Dam on PCV2 Replication In Utero. Clinical and Vaccine Immunology. 2009; 16(6): 830–834. https://doi.org/10.1128/CVI.00455-08Madson D.M., Opriessnig T. Effect of porcine circovirus type 2 (PCV2) infection on reproduction: disease, vertical transmission, diagnostics and vaccination. Animal Health Research Reviews. 2011; 12(1): 47–65. https://doi.org/10.1017/S1466252311000053Dal Santo A.C., Cezario K.C., Bennemann P.E., Machado S.A., Martins M. Full-genome sequences of porcine circovirus 3 (PCV3) and high prevalence in mummified fetuses from commercial farms in Brazil. Microbial Pathogenesis. 2020; 141: 104027. https://doi.org/10.1016/j.micpath.2020.104027Uribe-García H.F., Suarez-Mesa R.A., Rondón-Barragán I.S. Survey of porcine circovirus type 2 and parvovirus in swine breeding herds of Colombia. Veterinary Medicine and Science. 2022; 8(6): 2451–2459. https://doi.org/10.1002/vms3.949Tummaruk P., Pearodwong P. Expression of PCV2 antigen in the ovarian tissues of gilts. Journal of Veterinary Medical Science. 2016; 78(3): 457–461. https://doi.org/10.1292/jvms.15-0450Gauger P.C. et al. Leukogram abnormalities in gnotobiotic pigs infected with porcine circovirus type 2. Veterinary Microbiology. 2011; 154(1–2): 185–190. https://doi.org/10.1016/j.vetmic.2011.06.016Madson D.M., Opriessnig T. Effect of porcine circovirus type 2 (PCV2) infection on reproduction: disease, vertical transmission, diagnostics and vaccination. Animal Health Research Reviews. 2011; 12(1): 47–65. https://doi.org/10.1017/S1466252311000053Дерхо М.А., Бурков П.В., Щербаков П.Н. Оценка информативности методов диагностики поствакцинального иммунитета у свиней. Актуальные вопросы ветеринарных и сельскохозяйственных наук: теория и практика. Материалы Всероссийской национальной научной конференции. Челябинск: Южно-Уральский государственный аграрный университет. 2022; 39–45. https://elibrary.ru/hjducvUribe-García H.F., Suarez-Mesa R.A., Rondón-Barragán I.S. Survey of porcine circovirus type 2 and parvovirus in swine breeding herds of Colombia. Veterinary Medicine and Science. 2022; 8(6): 2451–2459. https://doi.org/10.1002/vms3.949Pearodwong P., Srisuwatanasagul S., Teankum K., Tantilertcharoen R., Tummaruk P. Prevalence of porcine circovirus-2 DNA-positive ovarian and uterine tissues in gilts culled due to reproductive disturbance in Thailand. Tropical Animal Health and Production. 2015; 47(5): 833–840. https://doi.org/10.1007/s11250-015-0796-5Gauger P.C. et al. Leukogram abnormalities in gnotobiotic pigs infected with porcine circovirus type 2. Veterinary Microbiology. 2011; 154(1–2): 185–190. https://doi.org/10.1016/j.vetmic.2011.06.016OʼConnor B. et al. Multiple porcine circovirus 2-associated abortions and reproductive failure in a multisite swine production unit. The Canadian Veterinary Journal. 2001; 42(7): 551–553.Derkho M.A., Burkov P.V., Shcherbakov P.N. Evaluation of the informativeness of methods for diagnosing post-vaccination immunity in pigs. Topical issues of veterinary and agricultural sciences: theory and practice. Proceedings of the All-Russian National Scientific Conference. Chelyabinsk: South Ural State Agrarian University. 2022; 39–45 (In Russian). https://elibrary.ru/hjducvPark J.-S. et al. Birth Abnormalities in Pregnant Sows Infected Intranasally with Porcine Circovirus 2. Journal of Comparative Pathology. 2005; 132(2–3): 139–144. https://doi.org/10.1016/j.jcpa.2004.09.003Pearodwong P., Srisuwatanasagul S., Teankum K., Tantilertcharoen R., Tummaruk P. Prevalence of porcine circovirus-2 DNA-positive ovarian and uterine tissues in gilts culled due to reproductive disturbance in Thailand. Tropical Animal Health and Production. 2015; 47(5): 833–840. https://doi.org/10.1007/s11250-015-0796-5Mateusen B., Maes D.G.D., Van Soom A., Lefebvre D., Nauwynck H.J. Effect of a porcine circovirus type 2 infection on embryos during early pregnancy. Theriogenology. 2007; 68(6): 896–901. https://doi.org/10.1016/j.theriogenology.2007.07.014OʼConnor B. et al. Multiple porcine circovirus 2-associated abortions and reproductive failure in a multisite swine production unit. The Canadian Veterinary Journal. 2001; 42(7): 551–553.Татоян М.Р. Особенности развития печени в эмбриогенезе свиней. Медицинская наука Армении. 2015; 55(4): 47–51.Park J.-S. et al. Birth Abnormalities in Pregnant Sows Infected Intranasally with Porcine Circovirus 2. Journal of Comparative Pathology. 2005; 132(2–3): 139–144. https://doi.org/10.1016/j.jcpa.2004.09.003Стяжкина С.Н., Ситников В.А., Кашапова Г.А., Данилова К.Н. Анатомия и физиология свиной селезенки и ее значимость в медицине. Studnet. 2021; 4(5): 8. https://elibrary.ru/mngnawMateusen B., Maes D.G.D., Van Soom A., Lefebvre D., Nauwynck H.J. Effect of a porcine circovirus type 2 infection on embryos during early pregnancy. Theriogenology. 2007; 68(6): 896–901. https://doi.org/10.1016/j.theriogenology.2007.07.014Che T. et al. Long non-coding RNAs and mRNAs profiling during spleen development in pig. PLoS ONE. 2018; 13(3): e0193552. https://doi.org/10.1371/journal.pone.0193552Tatoyan M.R. Features of the liver development in embryogenesis of pigs. Medical science of Armenia. 2015; 55(4): 47–51 (In Russian).Hotamisligil G.S. Inflammation, metaflammation and immunometabolic disorders. Nature. 2017; 542(7640): 177–185. https://doi.org/10.1038/nature21363Styazhkina S.N., Sitnikov V.A., Danilova K.N., Kashapova G.A. Anatomy and physiology of the pig spleen and its significance in medicine. Studnet. 2021; 4(5): 8 (In Russian). https://elibrary.ru/mngnawStrawn M., Behura S.K. Epigenetic regulation of fetal brain development in pig. Gene. 2022; 844: 146823. https://doi.org/10.1016/j.gene.2022.146823Che T. et al. Long non-coding RNAs and mRNAs profiling during spleen development in pig. PLoS ONE. 2018; 13(3): e0193552. https://doi.org/10.1371/journal.pone.0193552Schachtschneider K.M., Madsen O., Park C., Rund L.A., Groenen M.A.M., Schook L.B. Adult porcine genome-wide DNA methylation patterns support pigs as a biomedical model. BMC Genomics. 2015; 16: 743. https://doi.org/10.1186/s12864-015-1938-xHotamisligil G.S. Inflammation, metaflammation and immunometabolic disorders. Nature. 2017; 542(7640): 177–185. https://doi.org/10.1038/nature21363Коган Я.Э. Патология пуповины и ее роль в перинатальных осложнениях. Практическая медицина. 2016; 1(93): 22–25. https://elibrary.ru/vkwzctStrawn M., Behura S.K. Epigenetic regulation of fetal brain development in pig. Gene. 2022; 844: 146823. https://doi.org/10.1016/j.gene.2022.146823Wu G. et al. Functional amino acids in the development of the pig placenta. Molecular Reproduction and Development. 2017; 84(9): 870–882. https://doi.org/10.1002/mrd.22809Schachtschneider K.M., Madsen O., Park C., Rund L.A., Groenen M.A.M., Schook L.B. Adult porcine genome-wide DNA methylation patterns support pigs as a biomedical model. BMC Genomics. 2015; 16: 743. https://doi.org/10.1186/s12864-015-1938-xZhao Z. et al. Zika virus causes placental pyroptosis and associated adverse fetal outcomes by activating GSDME. eLife. 2022; 11: e73792. https://doi.org/10.7554/eLife.73792Kogan Ya.E. Umbilical cord pathology and its role in perinatal complications. Practical medicine. 2016; 1(93): 22–25 (In Russian). https://elibrary.ru/vkwzctDerkho M.A., Burkov P.V., Scherbakov P.N., Rebezov M.B., Stepanova K.V., Ansori A.M. Contribution of some immunological and metabolic factors to formation of piglets’ post-vaccination immunity. Теория и практика переработки мяса. 2022; 7(3): 193–199. https://doi.org/10.21323/2414-438X-2022-7-3-193-199Wu G. et al. Functional amino acids in the development of the pig placenta. Molecular Reproduction and Development. 2017; 84(9): 870–882. https://doi.org/10.1002/mrd.22809Oliver-Ferrando S. et al. Exploratory field study on the effect of Porcine circovirus 2 (PCV2) sow vaccination on serological, virological and reproductive parameters in a PCV2 subclinically infected sow herd. BMC Veterinary Research. 2018; 14: 130. https://doi.org/10.1186/s12917-018-1452-xZhao Z. et al. Zika virus causes placental pyroptosis and associated adverse fetal outcomes by activating GSDME. eLife. 2022; 11: e73792. https://doi.org/10.7554/eLife.73792Хамитов М.Р. Комплекс гистологических изменений в плаценте при цирковирусной инфекции свиней в специализированных предприятиях ОАО «Полевское» и ОАО «Сосновское». Аграрный вестник Урала. 2012; (5): 60–62. https://elibrary.ru/pakjcdDerkho M.A., Burkov P.V., Scherbakov P.N., Rebezov M.B., Stepanova K.V., Ansori A.M. Contribution of some immunological and metabolic factors to formation of piglets’ post-vaccination immunity. Theory and practice of meat processing. 2022; 7(3): 193–199. https://doi.org/10.21323/2414-438X-2022-7-3-193-199Togashi K., Mawatari T., Mitobe S., Moriya S. Reproductive Losses Associated with Porcine Circovirus Type 2 in a Japanese Herd of Seronegative Sows. Journal of Veterinary Medical Science. 2011; 73(7): 941–944. https://doi.org/10.1292/jvms.10-0387Oliver-Ferrando S. et al. Exploratory field study on the effect of Porcine circovirus 2 (PCV2) sow vaccination on serological, virological and reproductive parameters in a PCV2 subclinically infected sow herd. BMC Veterinary Research. 2018; 14: 130. https://doi.org/10.1186/s12917-018-1452-xKaruppannan A.K. et al. Emergence of Porcine Circovirus 2 Associated Reproductive Failure in Southern India. Transboundary and Emerging Diseases. 2016; 63(3): 314–320. https://doi.org/10.1111/tbed.12276Khamitov M.R. Complex of histological changes in the placenta when pigs circovirus infection in specialized interprises "Polevskoy" and "Sosnowskij". Agrarian Bulletin of the Urals. 2012; (5): 60–62 (In Russian). https://elibrary.ru/pakjcdHirai T., Nunoya T., Ihara T., Saitoh T., Shibuya K., Nakamura K. Infectivity of Porcine Circovirus 1 and Circovirus 2 in Primary Porcine Hepatocyte and Kidney Cell Cultures. Journal of Veterinary Medical Science. 2006; 68(2): 179–182. https://doi.org/10.1292/jvms.68.179Togashi K., Mawatari T., Mitobe S., Moriya S. Reproductive Losses Associated with Porcine Circovirus Type 2 in a Japanese Herd of Seronegative Sows. Journal of Veterinary Medical Science. 2011; 73(7): 941–944. https://doi.org/10.1292/jvms.10-0387Resendes A.R. et al. Apoptosis in postweaning multisystemic wasting syndrome (PMWS) hepatitis in pigs naturally infected with porcine circovirus type 2 (PCV2). The Veterinary Journal. 2011; 189(1): 72–76. https://doi.org/10.1016/j.tvjl.2010.06.018Karuppannan A.K. et al. Emergence of Porcine Circovirus 2 Associated Reproductive Failure in Southern India. Transboundary and Emerging Diseases. 2016; 63(3): 314–320. https://doi.org/10.1111/tbed.12276Sanchez R.E.Jr., Meerts P., Nauwynck H.J., Pensaert M.B. Change of porcine circovirus 2 target cells in pigs during development from fetal to early postnatal life. Veterinary Microbiology. 2003; 95(1–2): 15–25. https://doi.org/10.1016/s0378-1135(03)00120-2Hirai T., Nunoya T., Ihara T., Saitoh T., Shibuya K., Nakamura K. Infectivity of Porcine Circovirus 1 and Circovirus 2 in Primary Porcine Hepatocyte and Kidney Cell Cultures. Journal of Veterinary Medical Science. 2006; 68(2): 179–182. https://doi.org/10.1292/jvms.68.179Kim J., Chung H.-K., Jung T., Cho W.-S., Choi C., Chae C. Postweaning Multisystemic Wasting Syndrome of Pigs in Korea: Prevalence, Microscopic Lesions and Coexisting Microorganisms. Journal of Veterinary Medical Science. 2002; 64(1): 57–62. https://doi.org/10.1292/jvms.64.57Resendes A.R. et al. Apoptosis in postweaning multisystemic wasting syndrome (PMWS) hepatitis in pigs naturally infected with porcine circovirus type 2 (PCV2). The Veterinary Journal. 2011; 189(1): 72–76. https://doi.org/10.1016/j.tvjl.2010.06.018Jiang H. et al. Induction of Porcine Dermatitis and Nephropathy Syndrome in Piglets by Infection with Porcine Circovirus Type 3. Journal of Virology. 2019; 93(4): e02045–18. https://doi.org/10.1128/JVI.02045-18Sanchez R.E.Jr., Meerts P., Nauwynck H.J., Pensaert M.B. Change of porcine circovirus 2 target cells in pigs during development from fetal to early postnatal life. Veterinary Microbiology. 2003; 95(1–2): 15–25. https://doi.org/10.1016/s0378-1135(03)00120-2Deng H. et al. Histopathological Changes and Inflammatory Response in Specific Pathogen-Free (SPF) with Porcine Circovirus Type 3 Infection. Animals. 2023; 13(3): 530. https://doi.org/10.3390/ani13030530Kim J., Chung H.-K., Jung T., Cho W.-S., Choi C., Chae C. Postweaning Multisystemic Wasting Syndrome of Pigs in Korea: Prevalence, Microscopic Lesions and Coexisting Microorganisms. Journal of Veterinary Medical Science. 2002; 64(1): 57–62. https://doi.org/10.1292/jvms.64.57Zhang X. et al. Effect of porcine circovirus type 2 on the severity of lung and brain damage in piglets infected with porcine pseudorabies virus. Veterinary Microbiology. 2019; 237: 108394. https://doi.org/10.1016/j.vetmic.2019.108394Jiang H. et al. Induction of Porcine Dermatitis and Nephropathy Syndrome in Piglets by Infection with Porcine Circovirus Type 3. Journal of Virology. 2019; 93(4): e02045–18. https://doi.org/10.1128/JVI.02045-18Li X. et al. Coinfection of Porcine Circovirus 2 and Pseudorabies Virus Enhances Immunosuppression and Inflammation through NF-κB, JAK/STAT, MAPK, and NLRP3 Pathways. International Journal of Molecular Sciences. 2022; 23(8): 4469. https://doi.org/10.3390/ijms23084469Deng H. et al. Histopathological Changes and Inflammatory Response in Specific Pathogen-Free (SPF) with Porcine Circovirus Type 3 Infection. Animals. 2023; 13(3): 530. https://doi.org/10.3390/ani13030530Zhang X. et al. Effect of porcine circovirus type 2 on the severity of lung and brain damage in piglets infected with porcine pseudorabies virus. Veterinary Microbiology. 2019; 237: 108394. https://doi.org/10.1016/j.vetmic.2019.108394Zhang X. et al. Effect of porcine circovirus type 2 on the severity of lung and brain damage in piglets infected with porcine pseudorabies virus. Veterinary Microbiology. 2019; 237: 108394. https://doi.org/10.1016/j.vetmic.2019.108394Li X. et al. Coinfection of Porcine Circovirus 2 and Pseudorabies Virus Enhances Immunosuppression and Inflammation through NF-κB, JAK/STAT, MAPK, and NLRP3 Pathways. International Journal of Molecular Sciences. 2022; 23(8): 4469. https://doi.org/10.3390/ijms23084469Li X. et al. Coinfection of Porcine Circovirus 2 and Pseudorabies Virus Enhances Immunosuppression and Inflammation through NF-κB, JAK/STAT, MAPK, and NLRP3 Pathways. International Journal of Molecular Sciences. 2022; 23(8): 4469. https://doi.org/10.3390/ijms23084469

The authors declare that there are no conflicts of interest present.