vetpressАграрная наукаAgrarian science0869-81552686-701XРедакция журнала "Аграрная наука"10.32634/0869-8155-2023-374-9-52-58vetpress-2780Research ArticleЗООТЕХНИЯ И ВЕТЕРИНАРИЯZOOTECHNICS AND VETERINARY MEDICINEЭкспрессия ключевых генов в слепой кишке у кур линий СМ5 и СМ9 мясного кросса «Смена 9» на фоне замены рыбной мукиExpression of key genes in the caecum in CM5 and CM9 chickens of the meat cross «Smena 9» against the background of the replacement of fishmealhttps://orcid.org/0000-0002-5846-5105ЙылдырымЕ. А.YildirimE. A.

Елена Александровна Йылдырым, доктор биологических наук

бульвар Загребский, 19, корп. 1, Санкт-Петербург, 192288

Elena Alexandrovna Yildirim, Doctor of Biological Sciences

19 Zagrebskiy boulevard, 1 building, Saint-Petersburg, 192288

deniz@biotrof.ruhttps://orcid.org/0000-0003-2789-4844ИльинаЛ. А.IlinaL. A.

Лариса Александровна Ильина, доктор биологических наук

бульвар Загребский, 19, корп. 1, Санкт-Петербург, 192288

Larisa Alexandrovna Ilina, Doctor of Biological Sciences

19 Zagrebskiy boulevard, 1 building, Saint-Petersburg, 192288

ilina@biotrof.ruhttps://orcid.org/0000-0002-8795-6659ЛаптевГ. Ю.LaptevG. Y.

Георгий Юрьевич Лаптев, доктор биологических наук

бульвар Загребский, 19, корп. 1, Санкт-Петербург, 192288

George Yurievich Laptev, Doctor of Biological Sciences

19 Zagrebskiy boulevard, 1 building, Saint-Petersburg, 192288

laptev@biotrof.ruhttps://orcid.org/0000-0001-8789-9837ФилипповаВ. А.FilippovaV. A.

Валентина Анатольевна Филиппова, биотехнолог

бульвар Загребский, 19, корп. 1, Санкт-Петербург, 192288

Valentina Anatolievna Filippova, Biotechnologist

19 Zagrebskiy boulevard, 1 building, Saint-Petersburg, 192288

filippova@biotrof.ruhttps://orcid.org/0000-0001-8424-4114ДубровинА. В.DubrovinA. V.

Андрей Валерьевич Дубровин, кандидат ветеринарных наук

бульвар Загребский, 19, корп. 1, Санкт-Петербург, 192288

Andrey Valerievich Dubrovin, Candidate of Veterinary Sciences

19 Zagrebskiy boulevard, 1 building, Saint-Petersburg, 192288

dubrowin.a.v@yandex.ruhttps://orcid.org/0000-0001-9001-2432ТюринаД. Г.TurinaD. G.

Дарья Георгиевна Тюрина, кандидат экономических наук

бульвар Загребский, 19, корп. 1, Санкт-Петербург, 192288

Darya Georgievna Tiurina, Candidate of Economic Sciences

19 Zagrebskiy boulevard, 1 building, Saint-Petersburg, 192288

tiurina@biotrof.ruhttps://orcid.org/0000-0002-9541-6839КалиткинаК. А.KalitkinaK. A.

Ксения Андреевна Калиткина, биотехнолог

бульвар Загребский, 19, корп. 1, Санкт-Петербург, 192288

Ksenya Andreevna Kalitkina, Biotechnologist

19 Zagrebskiy boulevard, 1 building, Saint-Petersburg, 192288

ksenya.k.a@biotrof.ruhttps://orcid.org/0009-0005-1879-7497ДубровинаА. С.DubrovinaA. S.

Алиса Сергеевна Дубровина, биотехнолог

бульвар Загребский, 19, корп. 1, Санкт-Петербург, 192288

Alisa Sergeevna Dubrovina, Biotechnologist

19 Zagrebskiy boulevard, 1 building, Saint-Petersburg, 192288

dasvet@biotrof.ruhttps://orcid.org/0000-0002-4336-8273ПономареваЕ. С.PonomarevaE. S.

Екатерина Сергеевна Пономарева, биотехнолог

бульвар Загребский, 19, корп. 1, Санкт-Петербург, 192288

Ekaterina Sergeevna Ponomareva, Biotechnologist

19 Zagrebskiy boulevard, 1 building, Saint-Petersburg, 192288

kate@biotrof.ruhttps://orcid.org/0000-0003-0081-6336ФисининВ. И.FisininV. I.

Владимир Иванович Фисинин, академик Российской академии наук, доктор сельскохозяйственных наук, профессор, научный руководитель 

ул. Птицеградская, 10, Сергиев Посад, 141311

Vladimir Ivanovich Fisinin, Academician of the Russian Academy of Sciences, Doctor of Agricultural Sciences, Professor, Scientific Supervisor

10 Ptitsegradskaya Str., Sergiev Posad, 141311

olga@vnitip.ruhttps://orcid.org/0000-0001-9122-9553ЕгоровИ. А.EgorovI. A.

Иван Афанасьевич Егоров, академик Российской академии наук, доктор биологических наук, профессор, руководитель отдела исследований по кормлению птицы

ул. Птицеградская, 10, Сергиев Посад, 141311

Ivan Afanasievich Egorov, Academician of the Russian Academy of Sciences, Doctor of Biological Sciences, Professor, Head of the Poultry Feeding Research Department

10 Ptitsegradskaya Str., Sergiev Posad, 141311

olga@vnitip.ruhttps://orcid.org/0000-0002-5102-2248ЕгороваТ. А.EgorovaT. A.

Татьяна Анатольевна Егорова, доктор сельскохозяйственных наук, заместитель директора по научно-исследовательской работе

ул. Птицеградская, 10, Сергиев Посад, 141311

Tatiana Anatolievna Egorova, Doctor of Agricultural Sciences, Deputy Director for Research

10 Ptitsegradskaya Str., Sergiev Posad, 141311

eta164@yandex.ruhttps://orcid.org/0000-0003-4564-4427МанукянВ. А.ManukyanV. A.

Вардгес Агавардович Манукян, доктор сельскохозяйственных наук, главный научный сотрудник, заведующий отделом питания птицы

ул. Птицеградская, 10, Сергиев Посад, 141311

Vardges Agavardovich Manukyan, Doctor of Agricultural Sciences, Senior Researcher, Head of the Department of Poultry Nutrition

10 Ptitsegradskaya Str., Sergiev Posad, 141311

manukyan@vnitip.ruhttps://orcid.org/0000-0001-8391-5000ЛенковаТ. Н.LenkovaT. N.

Татьяна Николаевна Ленкова, доктор сельскохозяйственных наук, профессор, ученый секретарь

ул. Птицеградская, 10, Сергиев Посад, 141311

Tatyana Nikolaevna Lenkova, Doctor of Agricultural Sciences, Professor, Scientific Secretary

10 Ptitsegradskaya Str., Sergiev Posad, 141311

dissovet@vnitip.ruhttps://orcid.org/0000-0001-7243-7381ДегтяреваО. Н.DegtyarevaO. N.

Ольга Николаевна Дегтярева, кандидат сельскохозяйственных наук, научный сотрудник

ул. Птицеградская, 10, Сергиев Посад, 141311

Olga Nikolaevna Degtyareva, Candidate of Agricultural Sciences, Researcher

10 Ptitsegradskaya Str., Sergiev Posad, 141311

fncvnitip@mail.ruООО «БИОТРОФ+»Россия«BIOTROF+» LtdRussian FederationФедеральный научный центр «Всероссийский научно-исследовательский и технологический институт птицеводства»РоссияFederal State Budget Scientific Institution Federal Scientific Center «All-Russian Research and Technological Poultry Institute»Russian Federation202322092023095258Copyright © Йылдырым Е.А., Ильина Л.А., Лаптев Г.Ю., Филиппова В.А., Дубровин А.В., Тюрина Д.Г., Калиткина К.А., Дубровина А.С., Пономарева Е.С., Фисинин В.И., Егоров И.А., Егорова Т.А., Манукян В.А., Ленкова Т.Н., Дегтярева О.Н., 20232023Йылдырым Е.А., Ильина Л.А., Лаптев Г.Ю., Филиппова В.А., Дубровин А.В., Тюрина Д.Г., Калиткина К.А., Дубровина А.С., Пономарева Е.С., Фисинин В.И., Егоров И.А., Егорова Т.А., Манукян В.А., Ленкова Т.Н., Дегтярева О.Н.Yildirim E.A., Ilina L.A., Laptev G.Y., Filippova V.A., Dubrovin A.V., Turina D.G., Kalitkina K.A., Dubrovina A.S., Ponomareva E.S., Fisinin V.I., Egorov I.A., Egorova T.A., Manukyan V.A., Lenkova T.N., Degtyareva O.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vetpress.ru/jour/article/view/2780Актуальность

Актуальность. Зависимость от рыбной муки сдерживает развитие птицеводства. Цель исследования — анализ экспрессии ключевых генов у родительского поголовья Gallus gallus L. линий СМ5 и СМ9 мясного кросса «Смена 9» на фоне рационов с заменой рыбной муки.

Методы

Методы. Проведены опыты на курах линий СМ5 и СМ9: группы 1А и 1Б получали основной рацион (ОР), 2А и 2Б — ОР с заменой рыбной муки продуктами переработки сои, 3А и 3Б — ОР с заменой рыбной муки продуктами переработки подсолнечника, 4А и 4Б — ОР с заменой рыбной муки продуктами переработки сои и подсолнечника в соотношении 50:50. Анализ экспрессии генов проводили с помощью количественной ПЦР с обратной транскрипцией.

Результаты

Результаты. Наиболее выраженные изменения в уровнях экспрессии ключевых генов на фоне замены рыбной муки на продукты переработки сои и подсолнечника касались материнской линии плимутрок СМ9 по сравнению с отцовской линией корниш СМ5. Так, экспрессия мРНК SOD в группах 2Б, 3Б и 4Б уменьшалась в 14,3–100 раз по сравнению с контролем 1Б (р ≤ 0,05), тогда как в опыте на линии СМ5 экспрессия гена SOD в опытных группах 2А и 3А уменьшалась не более чем в 3,5 раза по сравнению с контролем 1А (р ≤ 0,05). Во всех опытных группах зоотехнические показатели мясной и яичной продуктивности отмечались на уровне контрольных групп (р > 0,05).

Relevance

Relevance. Dependence on fishmeal hinders the development of the poultry industry. The aim of the study was to analyze the expression of key genes in the parent stock of Gallus gallus L. lines CM5 and CM9 of the meat cross «Smena 9» on the background of diets with the replacement of fish meal.

Methods

Methods. Experiments were carried out on CM5 and CM9 chickens: groups 1A and 1B received the main diet (MD), 2A and 2B — MD with the replacement of fish meal with soy products, 3A and 3B — MD with the replacement of fish meal with sunflower products, 4A and 4B — MD with the replacement of fishmeal with soybean and sunflower products at a ratio of 50:50. Gene expression analysis was performed using quantitative reverse transcription PCR.

Results

Results. The most pronounced changes in the levels of expression of key genes against the background of the replacement of fishmeal with soybean and sunflower processed products concerned the maternal line Plymouth Rock CM9 compared to the paternal line Cornish CM5. Thus, the expression of SOD mRNA in groups 2B, 3B and 4B decreased by 14.3–100 times compared with control 1B (р ≤ 0.05). Whereas in the experiment on the CM5 line, the expression of the SOD gene in the experimental groups 2A and 3A decreased by no more than 3.5 times compared with the control 1A (р ≤ 0.05). In all experimental groups, zootechnical indicators of meat and egg productivity were noted at the level of control groups (р > 0.05).  

«Смена 9»мясной кроссэкспрессия геновколичественная ПЦР с обратной транскрипциейрыбная мукасояподсолнечник«Smena 9»meat-type crossgene expressionquantitative reverse transcription PCRfishmealsoysunflowerИсследование выполнено за счет средств гранта Российского научного фонда № 22-66-00061 «Экспрессия генов продуктивности и резистентности кур нового отечественного кросса “Смена 9” и ее влияние на иммунитет, особенности реализации генетического потенциала продуктивности при разном энергоаминокислотном питании».The study was funded by the grant of the Russian Science Foundation No. 22-66-00061 «Expression of productivity and resistance genes in chickens of the new domestic cross “Smena 9” and its effect on immunity, features of the realization of the genetic potential of productivity with different energy amino acid nutrition».ReferencesChen Yu., Ma J., Huang H., Zhong H. 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The authors declare that there are no conflicts of interest present.