vetpressАграрная наукаAgrarian science0869-81552686-701XРедакция журнала "Аграрная наука"10.32634/0869-8155-2023-374-9-171-178vetpress-2802Research ArticleАГРОИНЖЕНЕРИЯ И ПИЩЕВЫЕ ТЕХНОЛОГИИAGROENGINEERING AND FOOD TECHNOLOGIESLonicera caerulea L.: Сравнительное метаболомное исследование полифенольного состава методом тандемной масс-спектрометрии четырех сортов дальневосточной жимолостиLonicera caerulea L. Comparative metabolomics study of polyphenolic composition by tandem mass spectrometry of four varieties of Far Eastern honeysucklehttps://orcid.org/0000-0002-9732-1649РазгоноваМ. П.RazgonovaM. P.

Майя Петровна Разгонова, кандидат технических наук, доцент

ул. Вавилова, 9, Владивосток, 690024;п. Аякс, 10, о. Русский, Владивосток, 690922

Mayya Petrovna Razgonova, candidate of technical sciences, Associate Professor

9 Vavilova Str., Vladivostok, 690024;10, Ajax Bay, Russky Island, Vladivostok, 690922

https://orcid.org/0009-0007-8798-6464СабитовА. Ш.SabitovA. Sh.

Андрей Шамильевич Сабитов, кандидат сельскохозяйственных наук

ул. Вавилова, 9, Владивосток, 690024

Andrey Shamilievich Sabitov, Candidate of Agricultural Sciences

9 Vavilova Str., Vladivostok, 690024

andrsabitov@rambler.ruhttps://orcid.org/0000-0003-0580-3235СенотрусоваТ. А.SenotrusovaT. A.

Тамара Алексеевна Сенотрусова, кандидат технических наук, доцент

п. Аякс, 10, о. Русский, Владивосток, 690922

Tamara Alekseevna Senotrusova, Candidate of Technical Sciences, Associate Professor

10, Ajax Bay, Russky Island, Vladivostok, 690922

Senotrusova.tale@dvfu.ruhttps://orcid.org/0000-0002-4643-2250ЛиН. Г.LeeN. G.

Наталья Гаврошевна Ли, кандидат технических наук, доцент

п. Аякс, 10, о. Русский, Владивосток, 690922

Natalya Gavroshevna Lee, Candidate of Technical Sciences, Associate Professor

10, Ajax Bay, Russky Island, Vladivostok, 690922

li.ng@dvfu.ruМурзинаО. Г.MurzinaO. G.

Ольга Геннадьевна Мурзина, научный сотрудник

ул. Центральная, 4, пос. Сосновка, Петропавловск-Камчатский, 684033

Olga Gennadievna Murzina, Research Аssociate

4 Centralnaya Str., Sosnovka, Petropavlovsk-Kamchatsky, 684033

olyaska93@mail.ruРусаковаЕ. А.RusakovaE. A.

Елена Анатольевна Русакова, научный сотрудник

ул. Центральная, 4, пос. Сосновка, Петропавловск-Камчатский, 684033

Elena Anatolyevna Rusakova, Research Аssociate

4 Centralnaya Str., Sosnovka, Petropavlovsk-Kamchatsky, 684033

rubusarcticus@mail.ruПетрушаЕ. Н.PetrushaE. N.

Елена Николаевна Петруша, кандидат биологических наук, ведущий научный сотрудник

ул. Центральная, 4, пос. Сосновка, Петропавловск-Камчатский, 684033

Elena Nikolaevna Petrusha, Candidate of Biological Sciences, Leading Researcher

4 Centralnaya Str., Sosnovka, Petropavlovsk-Kamchatsky, 684033

khasbiullina@kamniish.ruhttps://orcid.org/0000-0001-7098-7662ТихоноваН. Г.TikhonovaN. G.

Надежда Геннадьевна Тихонова, кандидат биологических наук, ведущий научный сотрудник

ул. Большая Морская, 42/44, Санкт-Петербург, 190000

Nadezhda Gennadievna Tikhonova, Candidate of Biological Sciences, Leading Researcher

42/44 Bolshaya Morskaya Str., Saint-Petersburg, 190000

n.g.tikhonova@vir.nw.ruДальневосточная опытная станция — филиал ФИЦ «Всероссийский институт генетических ресурсов растений им. Н.И. Вавилова»; Дальневосточный федеральный университетРоссияFar Eastern Experimental Station — branch of the All-Russian Institute of Plant Genetic Resources named after N.I. Vavilov; Far Eastern Federal UniversityRussian FederationДальневосточная опытная станция — филиал ФИЦ «Всероссийский институт генетических ресурсов растений им. Н.И. Вавилова»РоссияFar Eastern Experimental Station — branch of the All-Russian Institute of Plant Genetic Resources named after N.I. VavilovRussian FederationДальневосточный федеральный университетРоссияFar Eastern Federal UniversityRussian FederationКамчатский научно-исследовательский институт сельского хозяйстваРоссияKamchatsky Scientific Research Institute of AgricultureRussian FederationФИЦ «Всероссийский институт генетических ресурсов растений им. Н.И. Вавилова»РоссияFederal Research Center «N.I. Vavilov All-Russian Institute of Plant Genetic Resources»Russian Federation20232709202309171178Copyright © Разгонова М.П., Сабитов А.Ш., Сенотрусова Т.А., Ли Н.Г., Мурзина О.Г., Русакова Е.А., Петруша Е.Н., Тихонова Н.Г., 20232023Разгонова М.П., Сабитов А.Ш., Сенотрусова Т.А., Ли Н.Г., Мурзина О.Г., Русакова Е.А., Петруша Е.Н., Тихонова Н.Г.Razgonova M.P., Sabitov A.S., Senotrusova T.A., Lee N.G., Murzina O.G., Rusakova E.A., Petrusha E.N., Tikhonova N.G.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vetpress.ru/jour/article/view/2802Актуальность

Актуальность. В статье приведено сравнительное метаболомное исследование ягод жимолости синей (L. caerulea), сортообразцы Волхова, Томичка, Голубое веретено, Амфора, представленные в коллекции Дальневосточной опытной станции ВИР.

Методы

Методы. Для определения комплекса вторичных метаболитов в экстрактах ягод жимолости синей использовали метод тандемной масс-спектрометрии. Для получения высококонцентрированных экстрактов применяли метод фракционной мацерации. В экстрактах ягод L. caerulea методом тандемной масс-спектрометрии достоверно идентифицировано 75 соединений полифенольной группы.

Результаты

Результаты. По результатам качественной идентификации компонентного состава экстрактов ягод жимолости синей построена диаграмма Венна, показывающая сходство и различие по полифенольным группам различных сортообразцов L. caerulea. Ягоды L. caerulea содержат много биологически активных веществ, в частности имеют большое количество соединений с антиоксидантной активностью. Выделенный полифенольный комплекс включал в себя соединения следующих групп: флавоны, флавонолы, флаван-3-олы, флаваноны, антоцианы, стильбены, кумарины, фенольные кислоты. Впервые в экстрактах ягод L. caerulea идентифицировано 30 химических соединений полифенольной группы (флавоны пентагидроксидиметоксифлавон, цирсилиол, яцеозидин, софораизофлавон А, дигидрокситетраметоксифлавон, хризоэриол-О-гексозид, формононетин-7-О-глюкозид, флаван-3-олы эпиафзелеxин, эпикатехин галлат, стильбены пиносильвин, ресвератрол, дигидроресвератрол, кумарины фраксетин, умбеллиферон и др.), не упоминавшихся ранее в исследованиях по L. caerulea.

Relevance

Relevance. The article presents a comparative metabolomic study of blue honeysuckle (L. caerulea) berries, varieties Volhova, Tomichka, Goluboe vereteno, Amfora, presented in the collection of the Far Eastern Experimental Station of N.I. Vavilov All-Russian Institute of Plant Genetic Resources.

Methods

Methods. To determine the complex of secondary metabolites in extracts of blue honeysuckle berries. used the method of tandem mass spectrometry. Fractional maceration was used to obtain highly concentrated extracts. In extracts of L. caerulea berries, 75 compounds of the polyphenol group were reliably identified by tandem mass spectrometry.

Results

Results. Based on the results of the qualitative identification of the component composition of blue honeysuckle berry extracts, a Venn diagram was constructed showing the similarity and difference in polyphenol groups of various L. caerulea varieties. Berries of L. caerulea contain a large amount of biologically active substances, in particular, they have a large number of compounds with antioxidant activity. The isolated polyphenol complex included compounds of the following groups: flavones, flavonols, flavan-3-ols, flavanones, anthocyanins, stilbenes, coumarins, phenolic acids. Also, for the first time in the extracts of L. caerulea berries, 30 chemical compounds of the polyphenol group were identified (flavones Pentahydroxydimethoxyflavone, Cirsiliol, jaceosidin, Sophoraisoflavone A, Dihydroxy tetramethoxyflavone, Chrysoeriol-O-hexoside, Formononetin-7-O-glucoside, flavon-3-ols (epi)-Afzelechin, (epi)-Catechin gallate, stilbenes Pinosylvin, Resveratrol, Dihydroresveratrol, coumarins Fraxetin, Umbelliferone, etc.), not previously mentioned in studies on L. caerulea.

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The authors declare that there are no conflicts of interest present.