vetpressАграрная наукаAgrarian science0869-81552686-701XРедакция журнала "Аграрная наука"10.32634/0869-8155-2024-379-2-53-60vetpress-2937Research ArticleВЕТЕРИНАРИЯVETERINARY MEDICINEВлияние различных источников аминокислот на состав кишечной микрофлоры мясных кур и петухов родительского стада кросса «Смена 9»Influence of various sources of amino acids on composition of intestinal microflora of meat chickens and roosters of parent herd of cross “Smena 9”https://orcid.org/0000-0002-5846-5105ЙылдырымЕ. А.YildirimE. A.

Елена Александровна Йылдырым –  доктор биологических наук 

бульвар Загребский, 19/1, Санкт-Петербург, 192284

Elena Alexandrovna Yildirim – Doctor of Biological Sciences 

19/1 Zagrebskiy Аve., Saint-Petersburg, 1192284

eniz@biotrof.ruhttps://orcid.org/0000-0003-2789-4844ИльинаЛ. А.IlyinaL. A.

Лариса Александровна Ильина – доктор биологических наук 

бульвар Загребский, 19/1, Санкт-Петербург, 192284

Larisa Alexandrovna Ilina –  Doctor of Biological Sciences 

19/1 Zagrebskiy Аve., Saint-Petersburg, 1192284

ilina@biotrof.ruhttps://orcid.org/0000-0002-8795-6659ЛаптевГ. Ю.LaptevG. Yu.

Георгий Юрьевич Лаптев –  доктор биологических наук 

бульвар Загребский, 19/1, Санкт-Петербург, 192284

George Yurievich Laptev –  Doctor of Biological Sciences

19/1 Zagrebskiy Аve., Saint-Petersburg, 1192284

laptev@biotrof.ruhttps://orcid.org/0000-0001-8789-9837ФилипповаВ. А.FilippovaV. A.

Валентина Анатольевна Филиппова – биотехнолог

бульвар Загребский, 19/1, Санкт-Петербург, 192284 

Valentina Anatolievna Filippova – Biotechnologist

 19/1 Zagrebskiy Аve., Saint-Petersburg, 1192284

filippova@biotrof.ruhttps://orcid.org/0000-0001-8424-4114ДубровинА. В.DubrovinA. V.

Андрей Валерьевич Дубровин – кандидат ветеринарных наук 

бульвар Загребский, 19/1, Санкт-Петербург, 192284

Andrey Valerievich Dubrovin – Candidate of Veterinary Sciences 

19/1 Zagrebskiy Аve., Saint-Petersburg, 1192284

dubrowin.a.v@yandex.ruТюринаД. Г.TyurinaD. G.

Дарья Георгиевна Тюрина – кандидат экономических наук

бульвар Загребский, 19/1, Санкт-Петербург, 192284

Darya Georgievna Tyurina – Candidate of Economic Sciences 

19/1 Zagrebskiy Аve., Saint-Petersburg, 1192284

tiurina@biotrof.ruhttps://orcid.org/0000-0002-9541-6839КалиткинаК. А.KalitkinaK. A.

Ксения Андреевна Калиткина –  биотехнолог 

бульвар Загребский, 19/1, Санкт-Петербург, 192284

Xenia Andreevna Kalitkina – Biotechnologist 

19/1 Zagrebskiy Аve., Saint-Petersburg, 1192284

ksenya.k.a@biotrof.ruhttps://orcid.org/0009-0005-1879-7497ДубровинаА. С.DubrovinaA. S.

Алиса Сергеевна Дубровина – биотехнолог 

бульвар Загребский, 19/1, Санкт-Петербург, 192284

Alisa Sergeevna Dubrovina – Biotechnologist 

19/1 Zagrebskiy Аve., Saint-Petersburg, 1192284

dasvet@biotrof.ruhttps://orcid.org/0000-0002-4336-8273ПономареваЕ. С.PonomarevaE. S.

Екатерина Сергеевна Пономарева – биотехнолог 

бульвар Загребский, 19/1, Санкт-Петербург, 192284

Ekaterina Sergeevna Ponomareva – Biotechnologist

19/1 Zagrebskiy Аve., Saint-Petersburg, 1192284

kate@biotrof.ruhttps://orcid.org/0000-0003-0081-6336ФисининВ. И.FisininV. I.

Владимир Иванович Фисинин – академик РАН, доктор сельскохозяйственных наук, профессор, научный руководитель

ул. Птицеградская, 10, Сергиев Посад, 141311

Vladimir Ivanovich Fisinin  – Academician of the Russian Academy of Sciences, Doctor of Agricultural Sciences, Professor, Scientific Supervisor

10 Ptitsegradskaya Str., Sergiev Posad, 141311

https://orcid.org/0000-0001-9122-9553ЕгоровИ. А.EgorovI. A.

Иван Афанасьевич Егоров – академик РАН, доктор биологических наук, профессор 

ул. Птицеградская, 10, Сергиев Посад, 141311

Ivan Afanasievich Egorov – Academician of the Russian Academy of Sciences, Doctor of Biological Sciences, Professor

10 Ptitsegradskaya Str., Sergiev Posad, 141311

https://orcid.org/0000-0002-5102-2248ЕгороваТ. А.EgorovaT. A.

Татьяна Анатольевна Егорова – доктор сельскохозяйственных наук, заместитель директора по научно-исследовательской работе

ул. Птицеградская, 10, Сергиев Посад, 141311

Tatiana Anatolievna Egorova – Doctor of Agricultural Sciences, Deputy Director for Research 

10 Ptitsegradskaya Str., Sergiev Posad, 141311

eta164@yandex.ruhttps://orcid.org/0000-0003-4564-4427МанукянВ. А.ManukyanV. A.

Вардгес Агавардович Манукян – доктор сельскохозяйственных наук, главный научный сотрудник, заведующий отделом питания птицы

 ул. Птицеградская, 10, Сергиев Посад, 141311

Vardges Agavardovich Manukyan – Doctor of Agricultural Sciences, Senior Researcher, Head of the Department of Poultry Nutrition

10 Ptitsegradskaya Str., Sergiev Posad, 141311

manukyan@vnitip.ruhttps://orcid.org/0000-0001-8391-5000ЛенковаТ. Н.LenkovaT. N.

Татьяна Николаевна Ленкова – доктор сельскохозяйственных наук, профессор, ученый секретарь 

ул. Птицеградская, 10, Сергиев Посад, 141311

Tatyana Nikolaevna Lenkova – Doctor of Agricultural Sciences, Professor, Scientific Secretary 

10 Ptitsegradskaya Str., Sergiev Posad, 141311

dissovet@vnitip.ruhttps://orcid.org/0000-0001-7243-7381ДегтяреваО. Н.DegtyarevaO. N.

Ольга Николаевна Дегтярева – кандидат сельскохозяйственных наук, научный сотрудник 

ул. Птицеградская, 10, Сергиев Посад, 141311

Olga Nikolaevna Degtyareva – Candidate of Agricultural Sciences, Researcher 

10 Ptitsegradskaya Str., Sergiev Posad, 141311

fncvnitip@mail.ruhttps://orcid.org/0000-0002-2911-5640ТишенковМ. С.TishenkovM. S.

Мария Сергеевна Тишенкова – младший научный сотрудник 

ул. Птицеградская, 10, Сергиев Посад, 141311

Maria Sergeevna Tishenkovа – Junior Researcher 

10 Ptitsegradskaya Str., Sergiev Posad, 141311

tishenkova.m@yandex.ruhttps://orcid.org/0000-0002-0108-2218ДемидоваЕ. С.DemidovaE. S.

Екатерина Сергеевна Демидова –  младший научный сотрудник 

ул. Птицеградская, 10, Сергиев Посад, 141311

Ekaterina Sergeevna Demidova – Junior Researcher 

10 Ptitsegradskaya Str., Sergiev Posad, 141311

mixalysha@mail.ruhttps://orcid.org/0009-0000-5100-4843КашпоровЛ. М.KashporovL. M.

Лев Михайлович Кашпоров – специалист

ул. Птицеградская, 10, Сергиев Посад, 141311

Lev Mikhailovich Kashporov – Specialist

10 Ptitsegradskaya Str., Sergiev Posad, 141311

lev-lud2@live.ruhttps://orcid.org/0000-0001-7484-196XПащенкоВ. Е.PashchenkoV. E.

Виктория Евгеньевна Пащенко –  аспирант, младший научный сотрудник 

ул. Птицеградская, 10, Сергиев Посад, 141311

Victoria Evgenievna Pashchenko – Graduate Student, Junior Researcher 

10 Ptitsegradskaya Str., Sergiev Posad, 141311

viktoriia_pashchenko@mail.ruООО «БИОТРОФ+»РоссияBIOTROF+ LtdRussian FederationФедеральный научный центр «Всероссийский научно-исследовательский и технологический институт птицеводства»РоссияAll-Russian Research and Technological Institute of Poultry of Russian Academy of SciencesRussian Federation202420022024025360Copyright © Йылдырым Е.А., Ильина Л.А., Лаптев Г.Ю., Филиппова В.А., Дубровин А.В., Тюрина Д.Г., Калиткина К.А., Дубровина А.С., Пономарева Е.С., Фисинин В.И., Егоров И.А., Егорова Т.А., Манукян В.А., Ленкова Т.Н., Дегтярева О.Н., Тишенков М.С., Демидова Е.С., Кашпоров Л.М., Пащенко В.Е., 20242024Йылдырым Е.А., Ильина Л.А., Лаптев Г.Ю., Филиппова В.А., Дубровин А.В., Тюрина Д.Г., Калиткина К.А., Дубровина А.С., Пономарева Е.С., Фисинин В.И., Егоров И.А., Егорова Т.А., Манукян В.А., Ленкова Т.Н., Дегтярева О.Н., Тишенков М.С., Демидова Е.С., Кашпоров Л.М., Пащенко В.Е.Yildirim E.A., Ilyina L.A., Laptev G.Y., Filippova V.A., Dubrovin A.V., Tyurina D.G., Kalitkina K.A., Dubrovina A.S., Ponomareva E.S., Fisinin V.I., Egorov I.A., Egorova T.A., Manukyan V.A., Lenkova T.N., Degtyareva O.N., Tishenkov M.S., Demidova E.S., Kashporov L.M., Pashchenko V.E.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vetpress.ru/jour/article/view/2937Актуальность

Актуальность. Кишечная микробиота играет решающую роль в переваривании корма и усвоении питательных веществ у сельскохозяйственной птицы, оказывая влияние на зоотехнические показатели. Цель исследования — сравнение влияния добавления в рацион лизина и метионина в различных формах на состав кишечной микрофлоры кур и петухов кросса «Смена 9», а также установление связи состава микрофлоры на разных рационах с зоотехническими показателями птиц.

Методы

Методы. Проведены физиологические исследования на мясных курах породы плимутрок и петухах породы корниш родительского стада отечественного кросса «Смена 9» селекции СГЦ «Смена». Были сформированы 4 группы (контрольная 1А и опытные 2А-4А) по 9 голов кур и 4 группы по 9 голов петухов (контрольная 1Б и опытные 2Б-4Б). Анализ проб содержимого слепых отростков кишечника птиц проводили методом ПЦР.

Результаты

Результаты. Как показал метод количественной ПЦР, изучаемые факторы кормления: различные источники лизина и метионина и сниженный на 5% уровень обменной энергии корма по-разному повлияли на состав микрофлоры кур и петухов родительского поголовья нового кросса «Смена 9». Так, например, при снижении уровня обменной энергии в рационах кур наблюдалось уменьшение от 1,2 до 5,0 раз таких представителей нормофлоры, как Bacteroidetes и Eubacteriaceae, по сравнению с аналогичными группами с базовым количеством обменной энергии (р ≤ 0,05). При введении в рацион лизина в форме монохлоргидрата и DL-метионина отмечено снижение массы яичников с яйцеводом на 6,9 г на фоне уменьшения уровня обменной энергии по сравнению с соответствующей группой с базовым содержанием обменной энергии (р ≤ 0,05), тогда как при использовании лизина в форме сульфата и метионина в форме гидроксианалога метионина подобного эффекта снижения не отмечено (р > 0,05). При этом сдвиги в составе микрофлоры на фоне изменения рационов не имели какой-либо связи с изученными зоотехническими параметрами у кур и петухов.

Relevance

Relevance. The intensin’s microbiota plays a crucial role in feed digestion and nutrient digestion in farm poultry, influencing zootechnical performance.

The aim of the study was to compare the effect of the addition of lysine and methionine in various forms to the diet on the composition of the intestinal microflora of chickens and roosters of the «Smena 9» cross, as well as to establish a connection between the composition of microflora in different diets with the zootechnical indicators of birds.

Methods

Methods. Physiological reseach were carried out on meat chickens of the Plimutrock breed and roosters of the Cornish breed of the parent herd of the domestic cross “Smena 9” selection of the “Smena” SSC. 4 groups were formed (control 1A and experimental 2A-4A) such as of 9 laying heads and 4 groups of 9 rooster heads (control 1B and experimental 2B-4B). Analysis of samples of the contents of blind processes of the intestine of birds was carried out by PCR.

Results

Results. As shown by the quantitative PCR method, the studied feeding factors: various sources of lysine and methionine and a 5% reduced level of metabolic energy of feed had a different effect on the composition of the microflora of chickens and roosters of the parent stock of the new cross «Smena 9». For example, with a decrease in the level of metabolic energy in the diets of chickens, there was a decrease from 1.2 to 5.0 times in such representatives of the normoflora as Bacteroidetes and Eubacteriaceae, compared with similar groups with a basic amount of metabolic energy (p < 0.05). When lysine was introduced into the diet in the form of monochlorohydrate and DL-methionine, a decrease in the weight of ovaries with an oviduct by 6.9 g was noted against the background of a decrease in the level of metabolic energy compared with the corresponding group with a base content of metabolic energy (p ≤ 0.05), whereas when using lysine in the form of sulfate and methionine in the form of a hydroxyanalog of methionine, a similar effect was reduced not noted (p > 0.05). At the same time, shifts in the composition of microflora against the background of changes in diets did not have any connection with the studied zootechnical parameters in chickens and roosters.

кросс «Смена 9»лизинметионининкубационные яйцамикрофлора кишечникаколичественная ПЦРcross «Smena 9»lysinemethionineincubation eggsintestinal microfloraquantitative PCRИсследования поддержаны грантом РНФ 22-66-00061 «Экспрессия генов продуктивности и резистентности кур нового отечественного кросса “Смена 9” и ее влияние на иммунитет, особенности реализации генетического потенциала продуктивности при разном энергоаминокислотном питании».Research supported by Russian Science Foundation grant 22-66-00061 «Expression of genes for productivity and resistance of chickens of the new domestic cross-section “Change 9” and its effect on immunity, features of the implementation of the genetic potential of productivity with different energyamino acid nutrition».ReferencesVieira S.L., Lemme A., Goldenberg D.B., Brugalli I. Responses of growing broilers to diets with increased sulfur amino acids to lysine ratios at two dietary protein levels. Poultry Science. 2004; 83(8): 1307–1313. https://doi.org/10.1093/ps/83.8.1307Vieira S.L., Lemme A., Goldenberg D.B., Brugalli I. Responses of growing broilers to diets with increased sulfur amino acids to lysine ratios at two dietary protein levels. Poultry Science. 2004; 83(8): 1307–1313. https://doi.org/10.1093/ps/83.8.1307Wickramasuriya S.S. et al. Differential Effects of Dietary Methionine Isomers on Broilers Challenged with Acute Heat Stress. The Journal of Poultry Science. 2019; 56(3): 195–203. https://doi.org/10.2141/jpsa.0180072Wickramasuriya S.S. et al. Differential Effects of Dietary Methionine Isomers on Broilers Challenged with Acute Heat Stress. The Journal of Poultry Science. 2019; 56(3): 195–203. https://doi.org/10.2141/jpsa.0180072Bauriedel W.R. The Effect of Feeding d-Methionine on the d-Amino Acid Oxidase Activity of Chick Tissues. Poultry Science. 1963; 42(1): 214–217. https://doi.org/10.3382/ps.0420214Bauriedel W.R. The Effect of Feeding d-Methionine on the d-Amino Acid Oxidase Activity of Chick Tissues. Poultry Science. 1963; 42(1): 214–217. https://doi.org/10.3382/ps.0420214Jackson M. A closer look at lysine sources: L-lysine sulfate plus fermentation co-products. Feed International. 2001; 22: 18–20.Jackson M. A closer look at lysine sources: L-lysine sulfate plus fermentation co-products. Feed International. 2001; 22: 18–20.Henry M.H. et al. The Performance of Broiler Chicks Fed Diets Containing Extruded Cottonseed Meal Supplemented with Lysine. Poultry Science. 2001; 80(1): 762–768. https://doi.org/10.1093/ps/80.6.762Henry M.H. et al. The Performance of Broiler Chicks Fed Diets Containing Extruded Cottonseed Meal Supplemented with Lysine. Poultry Science. 2001; 80(1): 762–768. https://doi.org/10.1093/ps/80.6.762Schutte J.B., Pack M. Biological efficacy of l-lysine preparations containing biomass compared to l-lysine-HCl. Archiv für Tierernaehrung. 1994; 46(3): 261–268. https://doi.org/10.1080/17450399409381775Schutte J.B., Pack M. Biological efficacy of l-lysine preparations containing biomass compared to l-lysine-HCl. Archiv für Tierernaehrung. 1994; 46(3): 261–268. https://doi.org/10.1080/17450399409381775Jia H. et al. Effects of L-lysine·H2SO4 product on the intestinal morphology and liver pathology using broiler model. Journal of Animal Science and Biotechnology. 2019; 10: 10. https://doi.org/10.1186/s40104-019-0318-9Jia H. et al. Effects of L-lysine·H2SO4 product on the intestinal morphology and liver pathology using broiler model. Journal of Animal Science and Biotechnology. 2019; 10: 10. https://doi.org/10.1186/s40104-019-0318-9Егорова А.В., Ефимов Д.Н., Емануйлова Ж.В., Комаров А.А. Эффект селекции отцовской линии породы корниш селекционно-генетического центра «Смена». Птицеводство. 2020; (3): 4–9. https://doi.org/10.33845/0033-3239-2020-69-3-4-9Egorova A.V., Efimov D.N., Emanuylova Zh.V., Komarov A.A. The effects of selection of paternal Cornish line of broiler breeders at the Center for Genetics & Selection “Smena”. Ptitsevodstvo. 2020; (3): 4–9 (in Russian). https://doi.org/10.33845/0033-3239-2020-69-3-4-9Егорова А.В. Основные направления работы с мясными курами родительского стада бройлеров. Птицеводство. 2017; (3): 16–21. https://elibrary.ru/ylkcfrEgorova A.V. The Principal Directions in Selection of Broiler Breeder Females. Ptitsevodstvo. 2017; (3): 16–21 (in Russian). https://elibrary.ru/ylkcfrКоноплева А.П. Ефимов Д.Н., Байковская Е.Ю., Емануйлова Ж.В. Воспроизводительные качества петухов отцовской линии СМ5 кросса «Смена 9». Птицеводство. 2021; (11): 16–20. https://doi.org/10.33845/0033-3239-2021-70-11-16-20Konopleva A.P., Efimov D.N., Baykovskaya E.Yu., Emanuylova Zh.V. The Reproductive Performance in Cornish Cocks of SM5 Line of Smena-9 Broiler Cross at 34-50 Weeks of Age. Ptitsevodstvo. 2021; (11): 16–20 (in Russian). https://doi.org/10.33845/0033-3239-2021-70-11-16-20Коноплева А.П. Эффективные приемы работы с петухами мясных кроссов в селекционных и родительских стадах. Птицеводство. 2021; (5): 43–49. https://doi.org/10.33845/0033-3239-2021-70-5-43-49Konopleva A.P. The Effective Techniques of Rearing and Management of Cocks of Broiler Crosses in Preparental and Parental Flocks. Ptitsevodstvo. 2021; (5): 43–49 (in Russian). https://doi.org/10.33845/0033-3239-2021-70-5-43-49Savory C.J., Lariviere J.-M. Effects of qualitative and quantitative food restriction treatments on feeding motivational state and general activity level of growing broiler breeders. Applied Animal Behaviour Science. 2000; 69(2): 135–147. https://doi.org/10.1016/s0168-1591(00)00123-4Savory C.J., Lariviere J.-M. Effects of qualitative and quantitative food restriction treatments on feeding motivational state and general activity level of growing broiler breeders. Applied Animal Behaviour Science. 2000; 69(2): 135–147. https://doi.org/10.1016/s0168-1591(00)00123-4de Jong I.C., Enting H., van Voorst A., Blokhuis H.J. Do low-density diets improve broiler breeder welfare during rearing and laying? Poultry Science. 2005; 84(2): 194–203. https://doi.org/10.1093/ps/84.2.194de Jong I.C., Enting H., van Voorst A., Blokhuis H.J. Do low-density diets improve broiler breeder welfare during rearing and laying? Poultry Science. 2005; 84(2): 194–203. https://doi.org/10.1093/ps/84.2.194Oviedo-Rondón E.O., Hume M.E., Hernández C., Clemente-Hernández S. Intestinal Microbial Ecology of Broilers Vaccinated and Challenged with Mixed Eimeria Species, and Supplemented with Essential Oil Blends. Poultry Science. 2006; 85(5): 854–860. https://doi.org/10.1093/ps/85.5.854Oviedo-Rondón E.O., Hume M.E., Hernández C., Clemente-Hernández S. Intestinal Microbial Ecology of Broilers Vaccinated and Challenged with Mixed Eimeria Species, and Supplemented with Essential Oil Blends. Poultry Science. 2006; 85(5): 854–860. https://doi.org/10.1093/ps/85.5.854Sugiharto S. Role of nutraceuticals in gut health and growth performance of poultry. Journal of the Saudi Society of Agricultural Sciences. 2016; 15(2): 99–111. https://doi.org/10.1016/j.jssas.2014.06.001Sugiharto S. Role of nutraceuticals in gut health and growth performance of poultry. Journal of the Saudi Society of Agricultural Sciences. 2016; 15(2): 99–111. https://doi.org/10.1016/j.jssas.2014.06.001Liu H.N. et al. Effects of dietary supplementation of quercetin on performance, egg quality, cecal microflora populations, and antioxidant status in laying hens. Poultry Science. 2014; 93(2): 347–353. https://doi.org/10.3382/ps.2013-03225Liu H.N. et al. Effects of dietary supplementation of quercetin on performance, egg quality, cecal microflora populations, and antioxidant status in laying hens. Poultry Science. 2014; 93(2): 347–353. https://doi.org/10.3382/ps.2013-03225Merciera Y., Francesch M., Badiola I., Pérez De Rozas A., Geraert P.-A. Effects of Methionine Sources and NSP Enzymes on Broiler Gut Microflora. 16th European Symposium on Poultry Nutrition. Proceedings. Strasbourg. 2007; 332.Merciera Y., Francesch M., Badiola I., Pérez De Rozas A., Geraert P.-A. Effects of Methionine Sources and NSP Enzymes on Broiler Gut Microflora. 16th European Symposium on Poultry Nutrition. Proceedings. Strasbourg. 2007; 332.Stadtman E.R., Van Remmen H., Richardson A., Wehr N.B., Levine R.L. Methionine oxidation and aging. Biochimica et Biophysica Acta (BBA) — Proteins and Proteomics. 2005; 1703(2): 135–140. https://doi.org/10.1016/j.bbapap.2004.08.010Stadtman E.R., Van Remmen H., Richardson A., Wehr N.B., Levine R.L. Methionine oxidation and aging. Biochimica et Biophysica Acta (BBA) — Proteins and Proteomics. 2005; 1703(2): 135–140. https://doi.org/10.1016/j.bbapap.2004.08.010Clarke S.F. et al. The gut microbiota and its relationship to diet and obesity. New insights. Gut Microbes. 2012; 3(3): 186–202. https://doi.org/10.4161/gmic.20168Clarke S.F. et al. The gut microbiota and its relationship to diet and obesity. New insights. Gut Microbes. 2012; 3(3): 186–202. https://doi.org/10.4161/gmic.20168van Der Wielen P.W.J.J., Biesterveld S., Notermans S., Hofstra H., Urlings B.A., van Knapen F. Role of Volatile Fatty Acids in Development of the Cecal Microflora in Broiler Chickens during Growth. Applied and Environmental Microbiology. 2000; 66(6): 2536–2540. https://doi.org/10.1128/AEM.66.6.2536-2540.2000van Der Wielen P.W.J.J., Biesterveld S., Notermans S., Hofstra H., Urlings B.A., van Knapen F. Role of Volatile Fatty Acids in Development of theDe Vadder F. et al. Microbiota-Generated Metabolites Promote Metabolic Benefits via Gut-Brain Neural Circuits. Cell. 2014; 156(1–2): 84–96. https://doi.org/10.1016/j.cell.2013.12.016Cecal Microflora in Broiler Chickens during Growth. Applied and Environmental Microbiology. 2000; 66(6): 2536–2540. https://doi.org/10.1128/AEM.66.6.2536-2540.2000Stanley D. et al. Identification of chicken intestinal microbiota correlated with the efficiency of energy extraction from feed. Veterinary Microbiology. 2013; 164(1–2): 85–92. https://doi.org/10.1016/j.vetmic.2013.01.030De Vadder F. et al. Microbiota-Generated Metabolites Promote Metabolic Benefits via Gut-Brain Neural Circuits. Cell. 2014; 156(1–2): 84–96. https://doi.org/10.1016/j.cell.2013.12.016Beam A., Clinger E., Hao L. Effect of Diet and Dietary Components on the Composition of the Gut Microbiota. Nutrients. 2021; 13(8): 2795. https://doi.org/10.3390/nu13082795Stanley D. et al. Identification of chicken intestinal microbiota correlated with the efficiency of energy extraction from feed. Veterinary Microbiology. 2013; 164(1–2): 85–92. https://doi.org/10.1016/j.vetmic.2013.01.030Erridge C., Attina T., Spickett C.M., Webb D.J. A high-fat meal induces lowgrade endotoxemia: evidence of a novel mechanism of postprandial inflammation. The American Journal of Clinical Nutrition. 2007; 86(5): 1286–1292. https://doi.org/10.1093/ajcn/86.5.1286Beam A., Clinger E., Hao L. Effect of Diet and Dietary Components on the Composition of the Gut Microbiota. Nutrients. 2021; 13(8): 2795. https://doi.org/10.3390/nu13082795Ghoshal S., Witta J., Zhong J., de Villiers W., Eckhardt E. Chylomicrons promote intestinal absorption of lipopolysaccharides. Journal of Lipid Research. 2009; 50(1): 90–97. https://doi.org/10.1194/jlr.M800156-JLR200Erridge C., Attina T., Spickett C.M., Webb D.J. A high-fat meal induces lowgrade endotoxemia: evidence of a novel mechanism of postprandial inflammation. The American Journal of Clinical Nutrition. 2007; 86(5): 1286–1292. https://doi.org/10.1093/ajcn/86.5.1286Kumar S., Adhikari P., Oakley B., Kim W.K. Changes in cecum microbial community in response to total sulfur amino acid (TSAA: DL-methionine) in antibioticfree and supplemented poultry birds. Poultry Science. 2019; 98(11): 5809–5819. https://doi.org/10.3382/ps/pez380Ghoshal S., Witta J., Zhong J., de Villiers W., Eckhardt E. Chylomicrons promote intestinal absorption of lipopolysaccharides. Journal of Lipid Research. 2009; 50(1): 90–97. https://doi.org/10.1194/jlr.M800156-JLR200Kollarcikova M. et al. Use of 16S rRNA gene sequencing for prediction of new opportunistic pathogens in chicken ileal and cecal microbiota. Poultry Science. 2019; 98(6): 2347–2353. https://doi.org/10.3382/ps/pey594Kumar S., Adhikari P., Oakley B., Kim W.K. Changes in cecum microbial community in response to total sulfur amino acid (TSAA: DL-methionine) in antibiotic-free and supplemented poultry birds. Poultry Science. 2019; 98(11): 5809–5819. https://doi.org/10.3382/ps/pez380Murphy E.C., Frick I.-M. Gram-positive anaerobic cocci — commensals and opportunistic pathogens. FEMS Microbiology Reviews. 2013; 37(4): 520–553. https://doi.org/10.1111/1574-6976.12005Kollarcikova M. et al. Use of 16S rRNA gene sequencing for prediction of new opportunistic pathogens in chicken ileal and cecal microbiota. Poultry Science. 2019; 98(6): 2347–2353.https://doi.org/10.3382/ps/pey594Yan L. et al. Effects of corn particle size on growth performance, gastrointestinal development, carcass indices and intestinal microbiota of broilers. Poultry Science. 2022; 101(12): 102205. https://doi.org/10.1016/j.psj.2022.102205Murphy E.C., Frick I.-M. Gram-positive anaerobic cocci — commensals and opportunistic pathogens. FEMS Microbiology Reviews. 2013; 37(4): 520–553. https://doi.org/10.1111/1574-6976.12005Dai D., Qi G.-h., Wang J., Zhang H.-j., Qiu K., Wu S.-g. Intestinal microbiota of layer hens and its association with egg quality and safety. Poultry Science. 2022; 101(9): 102008. https://doi.org/10.1016/j.psj.2022.102008Yan L. et al. Effects of corn particle size on growth performance, gastrointestinal development, carcass indices and intestinal microbiota of broilers. Poultry Science. 2022; 101(12): 102205. https://doi.org/10.1016/j.psj.2022.102205Liu T., Tang J., Feng F. Medium-chain α-monoglycerides improves productive performance and egg quality in aged hens associated with gut microbiota modulation. Poultry Science. 2020; 99(12): 7122–7132. https://doi.org/10.1016/j.psj.2020.07.049Dai D., Qi G.-h., Wang J., Zhang H.-j., Qiu K., Wu S.-g. Intestinal microbiota of layer hens and its association with egg quality and safety. Poultry Science. 2022; 101(9): 102008. https://doi.org/10.101 6/j.psj.2022.102008Feng J. et al. Dietary oregano essential oil supplementation improves intestinal functions and alters gut microbiota in late-phase laying hens. Journal of Animal Science and Biotechnology. 2021; 12: 72. https://doi.org/10.1186/s40104-021-00600-3Liu T., Tang J., Feng F. Medium-chain α-monoglycerides improves productive performance and egg quality in aged hens associated with gut microbiota modulation. Poultry Science. 2020; 99(12): 7122–7132. https://doi.org/10.1016/j.psj.2020.07.049Liu G. et al. Effects of Sex and Diet on Gut Microbiota of Farmland-Dependent Wintering Birds. Frontiers in Microbiology. 2020; 11: 587873. https://doi.org/10.3389/fmicb.2020.587873Feng J. et al. Dietary oregano essential oil supplementation improves intestinal functions and alters gut microbiota in late-phase laying hens. Journal of Animal Science and Biotechnology. 2021; 12: 72. https://doi.org/10.1186/s40104-021-00600-3Kers J.G., Velkers F.C., Fischer E.A.J., Hermes G.D.A., Stegeman J.A., Smidt H. Host and Environmental Factors Affecting the Intestinal Microbiota in Chickens. Frontiers in Microbiology. 2018; 9: 235. https://doi.org/10.3389/fmicb.2018.00235Liu G. et al. Effects of Sex and Diet on Gut Microbiota of FarmlandDependent Wintering Birds. Frontiers in Microbiology. 2020; 11: 587873. https://doi.org/10.3389/fmicb.2020.587873Kurata S., Hiradate Y., Umezu K., Hara K., Tanemura K. Capacitation of mouse sperm is modulated by gamma-aminobutyric acid (GABA) concentration. Journal of Reproduction and Development. 2019; 65(4): 327–334. https://doi.org/10.1262/jrd.2019-008Kers J.G., Velkers F.C., Fischer E.A.J., Hermes G.D.A., Stegeman J.A., Smidt H. Host and Environmental Factors Affecting the Intestinal Microbiota in Chickens. Frontiers in Microbiology. 2018; 9: 235. https://doi.org/10.3389/fmicb.2018.00235Fujinoki M., Takei G.L γ-Aminobutyric acid suppresses enhancement of hamster sperm hyperactivation by 5-hydroxytryptamine. Journal of Reproduction and Development. 2017; 63(1): 67–74. https://doi.org/10.1262/jrd.2016-091Kurata S., Hiradate Y., Umezu K., Hara K., Tanemura K. Capacitation of mouse sperm is modulated by gamma-aminobutyric acid (GABA) concentration. Journal of Reproduction and Development. 2019; 65(4): 327–334. https://doi.org/10.1262/jrd.2019-008Fujinoki M., Takei G.L γ-Aminobutyric acid suppresses enhancement of hamster sperm hyperactivation by 5-hydroxytryptamine. Journal of Reproduction and Development. 2017; 63(1): 67–74. https://doi.org/10.1262/jrd.2016-091Fujinoki M., Takei G.L γ-Aminobutyric acid suppresses enhancement of hamster sperm hyperactivation by 5-hydroxytryptamine. Journal of Reproduction and Development. 2017; 63(1): 67–74. https://doi.org/10.1262/jrd.2016-091

The authors declare that there are no conflicts of interest present.