Relevance. The problem of biological safety of drinking water remains relevant in livestock farming. The quality of water depends on the primary composition of the microflora of the source; with further transportation, the water is additionally contaminated with microorganisms. Infected water contributes to nutritional contamination of animals, the development of various pathologies of the gastrointestinal tract, the death of livestock, and a decrease in production indicators and enterprise profits. Currently, various methods of water purification have been proposed, but the search for the most effective methods and means of water disinfection continues. The purpose of the work is to evaluate the effectiveness of the drug “Di-O-Clean” as a source of chlorine dioxide for the disinfection of drinking water in industrial poultry farming. Methods. The methodology included bacteriological studies of 80 drinking water samples taken from various poultry farm facilities in different seasons of the year, a sanitary assessment of the poultry watering system after the use of “Di-O-Clean”. Results. As a result of the water tests, coliform bacteria and Pseudomonas aeruginosa were found. There was an increase in the number of microorganisms in the water of the storage tank and poultry watering system by 2.4 times compared to the amount of the initial microflora in the well water. The sanitary quality of water depends on the season of the year; the most unfavorable values of water assessment indicators were recorded in the spring. Production tests have shown that water disinfection with “Di-O-Clean” is effective, affordable, safe and economically feasible. Chlorine dioxide disinfection technology makes it possible to minimize or eliminate the transmission of infections through drinking water, raise healthy livestock and maintain the biological safety of the enterprise as a whole.