In the clinically healthy cows of the Holstein breed (n = 20), a hormonal background was studied during theperiod of deepness and after the edema. For this purpose, the concentration of kisspeptin, testosterone and cortisol was determined in serum cows in the blood cows. The animals conducted regular observation with the conduct of clinical and obstetric and gynecological studies. Depending on the concentration of glucose and beta-hydroxybutyric acid on the 5th day after calving, the cows were divided into two groups. The first group included 15 heads with a glucose concentration greater than 3 mmol/l and a beta-hydroxybutyric acid level below 1 mmol/l. In the second group, 5 heads had violations in energy exchange (glucose levels less than 3 mmol/l, beta-hydroxybutyric acid above 1 mmol/l). On the 15th day after the hotel, normalization of energy homeostasis was recorded. The concentration of kisspeptin in the preventive period in both groups decreased to the hotel. At the same time, the concentration of kisspeptin in the blood of animals in the group with energy exchange disorders was 2.4–3 times higher on the 9th, 7th and 5th days before calving (p < 0.01–0.05) compared with the first group. In production experience, the concentration of all three hormones after the hotel decreased with significant intergroup differences. The level of kisspeptin was reliably higher in the preventive period in the experimental group and on the 5th day after the hotel (p < 0.05). The cortisole level was held at a high level of up to 7 days after the hotel (p < 0.05). The concentration of testosterone in the blood throughout the transit period was reliably high in the animals of the experimental group.