vetpressАграрная наукаAgrarian science0869-81552686-701XРедакция журнала "Аграрная наука"10.32634/0869-8155-2024-385-8-82-87vetpress-3219Research ArticleЗООТЕХНИЯZOOTECHNICSИзменение метаболических параметров рубцового содержимого в результате преобразования отходов маслоэкстракционных производств в системе непрерывной ферментацииChanges in the metabolic parameters of rumen changes as a result of converting oil extraction waste into a continuous fermentation systemhttps://orcid.org/0000-0002-2586-613XШейдаЕ. В.SheidaE. V.

Елена Владимировна Шейда, научный сотрудник, кандидат биологических наук; старший научный сотрудник, кандидат биологических наук

ул. 9 Января, 29, Оренбург, 460000

пр-т Победы, 13, Оренбург, 460000

Elena Vladimirovna Sheida, Researcher, Candidate of Biological Sciences; Senior Researcher, Candidate of Biological Sciences

29 January 9th Str., Orenburg, 46000

13 Pobedy Ave., Orenburg, 460000

elena-shaejjda@mail.ruhttps://orcid.org/0000-0002-9015-8367ДускаевГ. К.DuskaevG. K.

Галимжан Калиханович Дускаев, ведущий научный сотрудник, доктор биологических наук

ул. 9 Января, 29, Оренбург, 460000

Galimzhan Kalihanovich Duskaev, Leading Researcher, Doctor of Biological Sciences

29 January 9th Str., Orenburg, 46000

gduskaev@mail.ruhttps://orcid.org/0000-0003-1173-1952МирошниковС. А.MiroshnikovS. A.

Сергей Александрович Мирошников, член-корреспондент РАН, доктор биологических наук

ул. 9 Января, 29, Оренбург, 460000

Sergey Aleksandrovich Miroshnikov, Corresponding Member of the Russian Academy of Sciences, Doctor of Biological Sciences

29 January 9th Str., Orenburg, 46000

fncbst@mail.ruhttps://orcid.org/0000-0003-1898-9307АринжановаМ. С.ArinzhanovaM. S.

Мария Сергеевна Аринжанова, младший научный сотрудник, аспирант

ул. 9 Января, 29, Оренбург, 460000

Maria Sergeevna Arinzhanova, Junior Researcher, Graduate Student

29 January 9th Str., Orenburg, 46000

marymiroshnikova@mail.ruhttps://orcid.org/0000-0001-7030-0912ПроскуринД. А.ProskurinD. A.

Дмитрий Александрович Проскурин, научный сотрудник, кандидат технических наук

ул. 9 Января, 29, Оренбург, 460000

Dmitry Aleksandrovich Proskurin, Researcher, Candidate of Technical Sciences

29 January 9th Str., Orenburg, 46000

dimitrpro@mail.ruФедеральный научный центр биологических систем и агротехнологий Российской академии наук; Оренбургский государственный университетРоссияFederal Scientific Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences; Orenburg State UniversityRussian FederationФедеральный научный центр биологических систем и агротехнологий Российской академии наукРоссияFederal Scientific Center for Biological Systems and Agrotechnologies of the Russian Academy of SciencesRussian Federation202423082024188287Copyright © Шейда Е.В., Дускаев Г.К., Мирошников С.А., Аринжанова М.С., Проскурин Д.А., 20242024Шейда Е.В., Дускаев Г.К., Мирошников С.А., Аринжанова М.С., Проскурин Д.А.Sheida E.V., Duskaev G.K., Miroshnikov S.A., Arinzhanova M.S., Proskurin D.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vetpress.ru/jour/article/view/3219

В данной работе авторы провели оценку метаболических параметров рубцового содержимого в результате преобразования растительного субстрата (льняной жмых) в конструкции биореактора (ферментере непрерывного действия).

Методы

Методы. Исследование проводили методом in vitro по специализированной методике с использованием биореактора, продолжительность ферментации в котором составляла 20 суток непрерывной ферментации без дополнительного добавления субстрата. Рубцовое содержимое было получено от бычков казахской белоголовой породы возрастом 15 месяцев с хронической фистулой рубца. Уровень летучих жирных кислот в содержимом рубца определялся методом газовой хроматографии. Определение химического состава испытуемого субстрата осуществлялось по общепринятым методикам.

Результаты

Результаты. Непрерывная ферментация льняного жмыха в биореакторе в течение 14 суток показала способность данного субстрата поддерживать активность рубцовой микробиоты для разложения питательных компонентов. В результате расщепления льняного жмыха отмечено сохранение достаточно высокого уровня летучих жирных кислот и метаболитов азота в инокуляте рубца, а также увеличение переваримости СВ, СЖ, СП в данном кормовом средстве. Результаты исследования показывают, что льняной жмых может быть использован для синтеза микробного белка и в качестве источника белка и жира при добавлении в рацион жвачных животных с использованием системы непрерывного культивирования.

In this paper, the authors evaluated the metabolic parameters of the scar content as a result of the transformation of a vegetable substrate (linseed cake) in the design of a bioreactor (continuous fermenter).

Methods

Methods. The study was carried out in vitro using a specialized technique using a bioreactor, the duration of fermentation in which was 20 days of continuous fermentation without additional addition of a substrate. The scar content was obtained from Kazakh white-headed bull calves aged 15 months with chronic scar fistula. The level of volatile fatty acids in the contents of the rumen was determined by gas chromatography. The chemical composition of the test substrate was determined according to generally accepted methods.

Results

Results. Continuous fermentation of linseed cake in a bioreactor for 14 days showed the ability of this substrate to maintain the activity of the scar microbiota for the decomposition of nutritional components. As a result of the splitting of flaxseed cake, the preservation of a sufficiently high level of volatile fatty acids and nitrogen metabolites in the rumen inoculum was noted, as well as an increase in the digestibility of DM, CF, CP in this feed medium, was noted. The results of this study show that flaxseed cake can be used for the synthesis of microbial protein and as a source of protein and fat when added to the diet of ruminants using a continuous cultivation system.

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The authors declare that there are no conflicts of interest present.