vetpressАграрная наукаAgrarian science0869-81552686-701XРедакция журнала "Аграрная наука"10.32634/0869-8155-2024-385-8-88-95vetpress-3220Research ArticleЗООТЕХНИЯZOOTECHNICSЭкспериментальная оценка взаимосвязи индигенного состава микрофлоры кишечника и элементного статуса карпа обыкновенного (Cyprinus caprio) на фоне применения новой кормовой добавкиExperimental assessment of the relationship between the indigenous composition of the intestinal microflora and the elemental status of the common carp (Cyprinus caprio) against the background of the use of new feed additivehttps://orcid.org/0000-0003-1099-3117СизенцовА. Н.SizentsovA. N.

Алексей Николаевич Сизенцов, доцент кафедры биохимии и микробиологии, кандидат биологических наук

пр-т Победы, 13, Оренбург, 460018

Aleksey Nikolaevich Sizentsov, Associate Professor of the Department of Biochemistry and Microbiology, Candidate of Biological Sciences

13 Pobedy Ave., Orenburg, 460018

asizen@mail.ruhttps://orcid.org/0000-0003-3804-5151МирошниковаЕ. П.MiroshnikovaE. P.

Елена Петровна Мирошникова, заведующая кафедрой биотехнологии животного сырья и аквакультуры, доктор биологических наук, профессор

пр-т Победы, 13, Оренбург, 460018

Elena Petrovna Miroshnikova, Head of the Department of Biotechnology of Animal Raw Materials and Aquaculture, Doctor of Biological Sciences, Professor 

13 Pobedy Ave., Orenburg, 460018

elenaakva@rambler.ruhttps://orcid.org/0000-0001-6534-7118АринжановА. Е.ArinzhanovA. E.

Азамат Ерсаинович Аринжанов, доцент кафедры биотехнологии животного сырья и аквакультуры, кандидат сельскохозяйственных наук

пр-т Победы, 13, Оренбург, 460018

Azamat Ersainovich Arinzhanov, Associate Professor of the Department of Biotechnology of Animal RawAzamat Ersainovich Arinzhanov Associate Professor of the Department of Biotechnology of Animal Raw Materials and Aquaculture, Candidate of Agricultural Sciences  Materials and Aquaculture, Candidate of Agricultural Sciences 

13 Pobedy Ave., Orenburg, 460018

arin.azamat@mail.ruКиляковаЮ. В.KilyakovaYu. V.

Юлия Владимировна Килякова, доцент кафедры биотехнологии животного сырья и аквакультуры, кандидат биологических наук

пр-т Победы, 13, Оренбург, 460018

Yulia Vladimirovna Kilyakova, Associate Professor of the Department of Biotechnology of Animal Raw Materials and Aquaculture, Candidate of Biological Sciences

13 Pobedy Ave., Orenburg, 460018

fish-ka06@mail.ruОренбургский государственный университетРоссияOrenburg State UniversityRussian Federation202423082024188895Copyright © Сизенцов А.Н., Мирошникова Е.П., Аринжанов А.Е., Килякова Ю.В., 20242024Сизенцов А.Н., Мирошникова Е.П., Аринжанов А.Е., Килякова Ю.В.Sizentsov A.N., Miroshnikova E.P., Arinzhanov A.E., Kilyakova Y.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vetpress.ru/jour/article/view/3220Актуальность

Актуальность. Аквакультура является интенсивно развивающейся отраслью во всем мире и представляет собой важный источник пищи для населения. Удовлетворение пищевых потребностей в микронутриентах является физиологически значимой частью питания рыб и, как следствие, обеспечивает их здоровье. В настоящее время применяются разные подходы для обеспечения устойчивости рыб к заболеваниям различной этиологии и повышения их продуктивности. Один из перспективных методических подходов — изучение микробиома рыб в качестве устойчивой альтернативы для улучшения методов аквакультуры.

Цель исследования — изучить степень взаимосвязи между составом микрофлоры кишечника и уровнем эссенциальных элементов в теле карпа (Cyprinus caprio) на фоне применения кормовых добавок «Бубитан» и «Интебио».

Методы

Методы. Объектом исследований являлись годовики карпа (n = 60), выращенные в условиях ООО «Ирикла-рыба» (Оренбургская обл.). В качестве регулирующих рост и развитие факторов использовали кормовые добавки «Интебио» и «Бутитан». Динамические показатели изменения видового состава микробиома кишечника и элементного статуса проводили с использованием методов метагеномного секвенирования, атомно-эмиссионной (АЭС-ИСП) и масс-спектрометрии (МС-ИСП).

Результаты

Результаты. Полученные данные свидетельствуют о значительном влиянии тестируемых добавок на показатели индигенной микрофлоры. Наиболее близкие по отношению к контролю показатели распределения таксономических групп микробиоценоза кишечника зарегистрированы на фоне применения кормовой добавки «Бутитан». Корреляционный анализ данных позволяет с высоким уровнем достоверности констатировать, что значительное увеличение численности микроорганизмов рода Hydrotalea и Flavobacterium оказывает положительное влияние на степень усвоения из корма макро- и микроэлементов.

Relevance

Relevance. Aquaculture is an intensively developing industry worldwide and is an important source of food for the population. Satisfaction of nutritional needs in micronutrients is a physiologically significant part of fish nutrition and, as a result, ensures their health. Currently, various approaches are being used to ensure the resistance of fish to diseases of various etiologies and to increase their productivity. One of the promising methodological approaches is the study of the fish microbiome as a sustainable alternative to improve aquaculture methods.

The aim of the study was to study the degree of relationship between the composition of the intestinal microflora and the level of essential elements in the body of carp (Cyprinus caprio) against the background of the use of feed additives “Bubitan” and “Intebio”.

Methods

Methods. The object of research was carp yearlings (n = 60) grown in the conditions of LLC “Irikla-fish” (Orenburg region). Feed additives “Intebio” and “Butitan” were used as growth and development regulating factors. Dynamic indicators of changes in the species composition of the intestinal microbiome and elemental status were carried out using metagenomic sequencing, atomic emission (NPP-ISP) and mass spectrometry (MS-ISP) methods.

Results

Results.The data obtained indicate a significant effect of the tested phytobiotics on the indices of indigenous microflora. The closest indicators of the distribution of taxonomic groups of intestinal microbiocenosis in relation to the control were registered against the background of the use of the phytobiotic «Butitan». Correlation analysis of the data allows us to state with a high level of reliability that a significant increase in the number of microorganisms of the genus Hydrotalea and Flavobacterium has a positive effect on the degree of assimilation of macro- and microelements from the feed.

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The authors declare that there are no conflicts of interest present.