vetpressАграрная наукаAgrarian science0869-81552686-701XРедакция журнала "Аграрная наука"10.32634/0869-8155-2024-385-8-132-138vetpress-3227Research ArticleЗООТЕХНИЯZOOTECHNICSВлияние внутриклеточного пероксида водорода на качественные показатели спермы петухов в цикле замораживания/оттаиванияIntracellular hydrogen peroxide’s effect on quality parameters of rooster sperm in freeze/thaw cycleКурочкинА. А.KurochkinA. A.

Антон Алексеевич Курочкин, младший научный сотрудник

Московское шоссе, 55А, пос. Тярлево, Пушкин, Санкт-Петербург, 196625

Anton Alekseevich Kurochkin, Junior Research Assistant 

55А Moskovskoe shosse, Tyarlevo village, Pushkin, St. Petersburg, 196625

kurochkin.anton.66@gmail.comhttps://orcid.org/0000-0002-4218-6080КузьминаТ. И.KuzminaT. I.

Татьяна Ивановна Кузьмина, главный научный сотрудник, профессор, доктор биологических наук

Московское шоссе, 55А, пос. Тярлево, Пушкин, Санкт-Петербург, 196625

Tatyana Ivanovna Kuzmina, Chief Researcher, Professor, Doctor of Biological Sciences

55А Moskovskoe shosse, Tyarlevo village, Pushkin, St. Petersburg, 196625

prof.kouzmina@mail.ruhttps://orcid.org/0000-0001-9504-3916СтанишевскаяО. И.StanishevskayaO. I.

Ольга Игоревна Станишевская, главный научный сотрудник, доктор биологических наук

Московское шоссе, 55А, пос. Тярлево, Пушкин, Санкт-Петербург, 196625

Olga Igorevna Stanishevskaya, Chief Researcher, Doctor of Biological Sciences

55А Moskovskoe shosse, Tyarlevo village, Pushkin, St. Petersburg, 196625

olgastan@list.ruВсероссийский научно-исследовательский институт генетики и разведения сельскохозяйственных животных — филиал Федерального исследовательского центра животноводства —ВИЖ им. академика Л.К. ЭрнстаРоссияRussian Research Institute of Farm Animal Genetics and Breeding — Branch of the L.K. Ernst Federal Research Center for Animal HusbandryRussian Federation20242308202418132138Copyright © Курочкин А.А., Кузьмина Т.И., Станишевская О.И., 20242024Курочкин А.А., Кузьмина Т.И., Станишевская О.И.Kurochkin A.A., Kuzmina T.I., Stanishevskaya O.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vetpress.ru/jour/article/view/3227Актуальность

Актуальность. Одним из факторов снижения общей подвижности после криоконсервации спермы петухов является влияние повышенной концентрации активных форм кислорода. Морфологические и биохимические особенности строения сперматозоидов птиц, делающие их более восприимчивыми к процессу криоконсервации по сравнению со сперматозоидами млекопитающих, могут также являться причиной, по которой сперма птиц больше подвержена влиянию оксидативного стресса.

Методы

Методы. Цели исследования — проследить изменение уровня внутриклеточных активных форм кислорода, в частности пероксида водорода (H2O2), в процессе криоконсервации спермы петухов, оценить его влияние на качественные показатели и жизнеспособность сперматозоидов.

Результаты

Результаты. Выявлена отрицательная корреляция (r = -0,68, p < 0,05) между внутриклеточным уровнем пероксида водорода и количеством мертвых клеток в нативной сперме. В замороженном (оттаянном) семени между данными показателями наблюдалась слабая взаимосвязь (r = -0,10). Наблюдалось достоверное влияние уровня внутриклеточного пероксида водорода в свежеполученных эякулятах на общую подвижность замороженного (оттаянного) семени (r = -0,65, р < 0,05). Это позволяет сделать предположение, что аналогично со сперматозоидами млекопитающих продуцирование клетками повышенного количества активных форм кислорода (H2O2) в цикле замораживания (оттаивания) негативно сказывается на функциональном статусе митохондрий, которые, как известно, являются основным источником энергии для сперматозоида, обеспечивающей работу кинетического аппарата сперматозоида и его общей подвижности. Были получены данные по индивидуальной изменчивости содержания пероксида водорода в сперматозоидах петухов в возрасте 61 недели в цикле замораживания (оттаивания), позволяющие вести отбор петухов по этому показателю.

Relevance

Relevance. One of the factors decreasing total motility after cryopreservation rooster’s sperm is influence of reactive oxygen species. Morphological and biochemical features of avian spermatozoa structure, which make them more susceptible to cryopreservation process compared to mammalian spermatozoa, may also be the reason why avian spermatozoa are more susceptible to oxidative stress.

Methods

Methods. The purpose of the study is to trace the change in the level of intracellular reactive oxygen species, in particular hydrogen peroxide (H2O2), during the cryopreservation of rooster sperm, to assess its effect on the quality and viability of sperm.

Results

Results. A negative correlation (r = -0.68, p < 0.05) was found between the intracellular level of hydrogen peroxide and the number of dead cells in native sperm. In the frozen (thawed) seed, a weak relationship was observed between these indicators (r = -0.10). There was a significant effect of the level of intracellular hydrogen peroxide in freshly obtained ejaculates on the overall mobility of frozen (thawed) semen (r = -0.65, p < 0.05). This allows us to assume that, similarly to mammalian spermatozoa, the production by cells of an increased amount of reactive oxygen species (H2O2) in the freezing (thawing) cycle negatively affects the functional status of mitochondria, which, as is known, are the main source of energy for the sperm, ensuring the operation of the kinetic apparatus of the sperm and its general mobility. Data were obtained on the individual variability of the hydrogen peroxide content in the sperm of roosters at the age of 61 weeks in the freezing (thawing) cycle, allowing the selection of roosters according to this indicator

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The authors declare that there are no conflicts of interest present.