vetpressАграрная наукаAgrarian science0869-81552686-701XРедакция журнала "Аграрная наука"10.32634/0869-8155-2024-386-9-40-47vetpress-3267Research ArticleВЕТЕРИНАРИЯVETERINARY MEDICINEЭкспериментальное исследование острой токсичности внутримышечной формы специфического иммунобиостимулятора — трансфер-факторExperimental study of acute toxicity of the intramuscular form of a specific immunobiostimulator — transfer factorhttps://orcid.org/0000-0001-7515-5670БурковП. В.BurkovP. V.

Павел Валерьевич Бурков, кандидат ветеринарных наук, руководитель научно-исследовательского центра биотехнологии репродукции животных

ул. им. Гагарина, 13, Троицк, Челябинская обл., 457100

Pavel Valerievich Burkov, Candidate of Veterinary Sciences, Head of the Research Center for Animal Reproduction Biotechnology

13 Gagarina Str., Troitsk, Chelyabinsk region, 457100

burcovpavel@mail.ruhttps://orcid.org/0000-0003-3818-0556ДерхоМ. А.DerkhoM. A.

Марина Аркадьевна Дерхо, доктор биологических наук, заведующая кафедрой естественнонаучных дисциплин

ул. им. Гагарина, 13, Троицк, Челябинская обл., 457100

Marina Arkadyevna Derkho, Doctor of Biological Sciences, Head of the Department of Natural Sciences

13 Gagarina Str., Troitsk, Chelyabinsk region, 457100

derkho2010@yandex.ruhttps://orcid.org/0000-0003-0857-5143РебезовМ. Б.RebezovM. B.

Максим Борисович Ребезов, доктор сельскохозяйственных наук, кандидат ветеринарных наук, профессор, главный научный сотрудник; доктор сельскохозяйственных наук, кандидат ветеринарных наук, профессор кафедры биотехнологии и пищевых продуктов

ул. им. Талалихина, 26, Москва, 109316

ул. им. Карла Либкнехта, 42, Екатеринбург, 620075

Maksim Borisovich Rebezov, Doctor of Agricultural Sciences, Candidate of Veterinary Sciences, Professor, Chief Researcher; Doctor of Agricultural Sciences, Candidate of Veterinary Sciences, Professor of the Department of Biotechnology and Food Products

26 Talalikhin Str., Moscow, 109316

42 Karl Liebknecht Str., Yekaterinburg, 620075

rebezov@ya.ruhttps://orcid.org/0000-0001-8685-4645ЩербаковП. Н.ShcherbakovP. N.

Павел Николаевич Щербаков, доктор ветеринарных наук, профессор кафедры инфекционных болезней и ветеринарно-санитарной экспертизы

ул. им. Гагарина, 13, Троицк, Челябинская обл., 457100

Pavel Nikolaevich Shcherbakov, Doctor of Veterinary Sciences, Professor of the Department of Infectious Diseases and Veterinary and Sanitary Expertise

13 Gagarina Str., Troitsk, Chelyabinsk region, 457100

scherbakov_pavel@mail.ruhttps://orcid.org/0000-0002-1914-8721ДерхоА. О.DerkhoA. O.

Арина Олеговна Дерхо, аспирант

ул. им. Гагарина, 13, Троицк, Челябинская обл., 457100

Arina Olegovna Derkho, Graduate student

13 Gagarina Str., Troitsk, Chelyabinsk region, 457100

arina_avrora@mail.ruЮжно-Уральский государственный аграрный университетРоссияSouth Ural State Agrarian UniversityRussian FederationФедеральный научный центр пищевых систем им. В.М. Горбатова Российской академии наук; Уральский государственный аграрный университетРоссияGorbatov Research center for Food Systems; Ural State Agrarian UniversityRussian Federation202411102024194047Copyright © Бурков П.В., Дерхо М.А., Ребезов М.Б., Щербаков П.Н., Дерхо А.О., 20242024Бурков П.В., Дерхо М.А., Ребезов М.Б., Щербаков П.Н., Дерхо А.О.Burkov P.V., Derkho M.A., Rebezov M.B., Shcherbakov P.N., Derkho A.O.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vetpress.ru/jour/article/view/3267Актуальность

Актуальность. Представлены данные о токсикологической оценке безопасности специфического иммунобиостимулятора антигеннаправленного действия «Трансфер-фактора» в форме, готовой для внутримышечного применения, и полученного из крови гипериммунизированных доноров, на лабораторных животных в остром эксперименте.

Методы

Методы. Объектом исследования явилась готовая форма «Трансфер-фактора», острая токсичность которого определена в моделях на лабораторных животных (нелинейные мыши, нелинейные крысы).

Результаты

Результаты. Установлено, что после введения препарата мышам внутримышечно (0,25 мл/гол) и внутрибрюшинно (0,5 мл/гол), крысам внутримышечно (2,5 мл/гол) и внутрибрюшинно (2,5 мл/гол) наблюдается тремор мышц, исчезающий через 15–20 мин. Внутрибрюшинное введение препарата мышам и крысам в дозировках 0,75 мл/гол и 3,75 мл/гол, соответственно, определяет наличие тремора длительностью 40 мин. В ходе 14-суточного периода наблюдений масса тела мышей и крыс увеличивается на 10,15–14,25% и 11,84–17,67%. При аутопсии животных не выявляются видимые изменения в расположении органов и жидкость в брюшной и плевральной полостях. Однако в легких, сердце, селезенке и печени обнаруживаются некоторые патологические изменения цвета, консистенции и размера при внутримышечном введении «Трансфер-фактора» мышам и крысам в дозировке 0,25 мл/гол и 2,5 мл/гол, внутрибрюшинном введении мышам в дозировке 0,5 мл/гол и 0,75 мл/гол и крысам 2,5 мл/гол и 3,75 мл/гол. По совокупности изменений жизненно важных признаков лабораторных животных, массы тела и макроскопических характеристик внутренних органов по ГОСТ 12.1.007-76 препарат «Трансфер-фактор» может быть отнесен к IV классу опасности «Вещества малоопасные».

Relevance

Relevance. The article presents data on the toxicological assessment of the safety of the specific immunobiostimulant of antigen-directed action “Transfer Factor” in a form ready for intramuscular administration and obtained from the blood of hyperimmunized donors on laboratory animals in an acute experiment.

Methods

Methods. The object of the study was the finished form of “Transfer Factor”, the acute toxicity of which was determined in models on laboratory animals (nonlinear mice, nonlinear rats).

Results

Results. It was found that after administration of the drug to mice intramuscularly (0.25 ml/head) and intraperitoneally (0.5 ml/head), to rats intramuscularly (2.5 ml/head) and intraperitoneally (2.5 ml/head), muscle tremor is observed, disappearing after 15–20 minutes. Intraperitoneal administration of the drug to mice and rats in dosages of 0.75 ml/head and 3.75 ml/head, respectively, determines the presence of tremor lasting 40 minutes. During the 14-day observation period, the body weight of mice and rats increases by 10.15-14.25 and 11.84-17.67%. Autopsy of animals does not reveal visible changes in the location of organs and fluid in the abdominal and pleural cavities. However, some pathological changes in color, consistency and size are found in the lungs, heart, spleen and liver with intramuscular administration of Transfer Factor to mice and rats at a dose of 0.25 ml/head and 2.5 ml/head, intraperitoneal administration to mice at a dose of 0.5 ml/head and 0.75 ml/head and to rats 2.5 ml/head and 3.75 ml/head. Based on the totality of changes in the vital signs of laboratory animals, body weight and macroscopic characteristics of internal organs according to GOST 12.1.007-76, the drug “Transfer Factor” can be classified as hazard class IV “Low-hazard substances”.

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The authors declare that there are no conflicts of interest present.