vetpressАграрная наукаAgrarian science0869-81552686-701XРедакция журнала "Аграрная наука"10.32634/0869-8155-2024-388-11-109-116vetpress-3354Research ArticleАГРОНОМИЯAGRONOMYВыявление гибридов Solanum tuberosum с высоким уровнем устойчивости к фитофторозуIdentification of promising Solanum tuberosum hybrids with high resistance to late blighthttps://orcid.org/0000-0002-4131-3965КазаковО. Г.KazakovO. G.

Казаков Олег Геннадьевич - кандидат сельскохозяйственных наук, старший научный сотрудник.

ул. Лорха, 23В, дач. пос. Красково, г. о. Люберцы, Московская обл., 140051

Oleg G. Kazakov - Candidate of Agricultural Sciences, Senior Research.

23В Lorkh Str., Kraskovo village, Lyubertsy, Moscow region, 140051

kazakov-og@yandex.ruhttps://orcid.org/0000-0002-3992-5452ПоливановаО. Б.PolivanovaO. B.

Поливанова Оксана Борисовна - кандидат биологических наук, научный сотрудник, ФИЦ картофеля им. А.Г. Лорха, РГАУ-МСХА им. К.А. Тимирязева.

ул. Лорха, 23В, дач. пос. Красково, г. о. Люберцы, Московская обл., 140051; ул. Тимирязевская, 49, Москва, 127550

Oksana B. Polivanova - Candidate of Biological Sciences, Researcher, Russian Potato Research Centre, Russian State Agrarian University — Moscow Timiryazev Agricultural Academy.

23В Lorkh Str., Kraskovo village, Lyubertsy, Moscow region, 140051; 49 Timiryazevskaya Str., Moscow, 127550

polivanovaoks@gmail.comhttps://orcid.org/0000-0002-4131-3965ДеревягинаМ. К.DerevyaginaM. K.

Деревягина Марина Константиновна - кандидат биологических наук, ведущий научный сотрудник.

ул. Лорха, 23В, дач. пос. Красково, г. о. Люберцы, Московская обл., 140051

Marina C. Derevyagina - Candidate of Biological Sciences, Leading Researcher.

23В Lorkh Str., Kraskovo village, Lyubertsy, Moscow region, 140051

vzeyruk@mail.ruhttps://orcid.org/0000-0001-7521-6883БирюковаВ. A.BiryukovaV. A.

Бирюкова Виктория Александровна - кандидат биологических наук, ведущий сотрудник.

ул. Лорха, 23В, дач. пос. Красково, г. о. Люберцы, Московская обл., 140051

Victoria A. Biryukova - Candidate of Biological Sciences, Senior Researcher.

23В Lorkh Str., Kraskovo village, Lyubertsy, Moscow region, 140051

vika_biryukova@inbox.ruФедеральный исследовательский центр картофеля им. А.Г. ЛорхаРоссияRussian Potato Research CentreRussian FederationФедеральный исследовательский центр картофеля им. А.Г. Лорха; Российский государственный аграрный университет — МСХА им. К.А. ТимирязеваРоссияRussian Potato Research Centre; Russian State Agrarian University — Moscow Timiryazev Agricultural AcademyRussian Federation202422112024011109116Copyright © Казаков О.Г., Поливанова О.Б., Деревягина М.К., Бирюкова В.A., 20242024Казаков О.Г., Поливанова О.Б., Деревягина М.К., Бирюкова В.A.Kazakov O.G., Polivanova O.B., Derevyagina M.K., Biryukova V.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vetpress.ru/jour/article/view/3354

Изучение генетического разнообразия картофеля на основе использования традиционных и современных молекулярно-генетических методов идентификации ценных генов позволяет ускорить выделение нужных генотипов с комплексом хозяйственно полезных признаков для использования в селекции. В данной работе по результатам многолетнего изучения 200 образцов питомника фитофтороустойчивых гибридов из генетической коллекции был выделен 31 образец со стабильно высоким проявлением признака по годам. Оценка производилась в полевых условиях и методом искусственного заражения клубней и листьев. Было определено наличие маркеров генов устойчивости методом ПЦР. По результатам проведенных испытаний данные образцы отличались высоким уровнем устойчивости листьев и клубней, характеризуются высоким уровнем ряда других селекционно значимых признаков. При этом отобранные образцы имели до 4 маркеров Rpi-генов, обусловливающих вертикальную устойчивость генотипов. Выявленные образцы могут быть использованы в селекционном процессе в качестве доноров высокого уровня устойчивости к фитофторозу.

The study of potato genetic diversity using traditional and modern molecular genetic methods for identifying valuable genes allows for faster selection of desired genotypes with a set of economically important traits for use in breeding programs. In this research, based on the results of a long-term study of 200 nursery samples of late blight-resistant hybrids from the genetic collection, 31 samples with a consistently high manifestation of the trait over the years were selected. The assessment was carried out in the field and by the method of artificial infection of tubers and leaves. The presence of markers of resistance genes was determined by PCR. According to the results of the tests, these samples were characterized by a high level of stability of leaves and tubers, and are characterized by a high level of a number of other selection-significant features. At the same time, the selected samples had up to 4 markers of Rpi genes, which determine the vertical resistance of genotypes. The identified samples can be used in the breeding process as donors of a high level of resistance to late blight.

картофельфитофторозPhytophthora infestans (Mont.) de Baryгоризонтальная устойчивостьRpi-генымолекулярные маркерыгенетическая коллекцияpotatolate blightPhytophthora infestans (Mont.) de Baryhorizontal resistanceRpi-genesmolecular markersgenetic collectionРабота выполнена в рамках государственного задания Федерального исследовательского центра картофеля им. А.Г. 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The authors declare that there are no conflicts of interest present.