vetpressАграрная наукаAgrarian science0869-81552686-701XРедакция журнала "Аграрная наука"10.32634/0869-8155-2025-391-02-87-94vetpress-3450Research ArticleЗООТЕХНИЯZOOTECHNICSВлияние различных форм лизина и метионина в рационе бройлеров на профиль транскрипции ключевых геновEffect of different forms of lysine and methionine in broiler diets on the key genes transcriptional profilehttps://orcid.org/0000-0002-5846-5105ЙылдырымЕ. А.YildirimE. A.

Елена Александровна Йылдырым - доктор биологических наук, главный биотехнолог молекулярно-генетической лаборатории; доктор биологических наук, профессор кафедры крупного животноводства.

Бульвар Загребский, 19/1, Санкт-Петербург, 192284; Петербургское шоссе, 2, Пушкин, Санкт-Петербург, 196601

Elena Alexandrovna Yildirim - Doctor of Biological Sciences, Chief Biotechnologist of the Molecular Genetic Laboratory; Doctor of Biological Sciences, Professor of the Department of Large Animal Husbandry.

19/1 Zagrebskiy Аve., Saint Petersburg, 1192284; Peterburgskoe Highway, Pushkin, St. Petersburg, 196601

deniz@biotrof.ruhttps://orcid.org/0000-0003-2789-4844ИльинаЛ. А.IlyinaL. A.

Лариса Александровна Ильина - доктор биологических наук, начальник молекулярно-генетической лаборатории; доктор биологических наук, профессор кафедры крупного животноводства.

Бульвар Загребский, 19/1, Санкт-Петербург, 192284; Петербургское шоссе, 2, Пушкин, Санкт-Петербург, 196601

Larisa Alexandrovna Ilyina - Doctor of Biological Sciences, Head of the Molecular Genetic Laboratory; Doctor of Biological Sciences, Professor of the Department of Large Animal Husbandry.

19/1 Zagrebskiy Аve., Saint Petersburg, 1192284; Peterburgskoe Highway, Pushkin, St. Petersburg, 196601

ilina@biotrof.ruhttps://orcid.org/0000-0002-8795-6659ЛаптевГ. Ю.LaptevG. Yu.

Георгий Юрьевич Лаптев - доктор биологических наук, генеральный директор ООО «БИОТРОФ+».

Бульвар Загребский, 19/1, Санкт-Петербург, 192284

Georgy Yurievich Laptev - Doctor of Biological Sciences, CEO of “BIOTROF+”.

19/1 Zagrebskiy Аve., Saint Petersburg, 1192284

Ltdlaptev@biotrof.ruhttps://orcid.org/0000-0001-8789-9837ФилипповаВ. А.FilippovaV. A.

Валентина Анатольевна Филиппова - старший биотехнолог молекулярно-генетической лаборатории; аведующая лабораторией кафедры крупного животноводства.

Бульвар Загребский, 19/1, Санкт-Петербург, 192284; Петербургское шоссе, 2, Пушкин, Санкт-Петербург, 196601

Valentina Anatolyevna Filippova - Senior Biotechnologist of the Molecular Genetic Laboratory; Head of the Laboratory of the Department of Large Animal Husbandry.

19/1 Zagrebskiy Аve., Saint Petersburg, 1192284; Peterburgskoe Highway, Pushkin, St. Petersburg, 196601

filippova@biotrof.ruhttps://orcid.org/0000-0001-8424-4114ДубровинА. В.DubrovinA. V.

Андрей Валерьевич Дубровин - кандидат ветеринарных наук, биотехнолог молекулярногенетической лаборатории; кандидат ветеринарных наук, старший научный сотрудник кафедры крупного животноводства.

Бульвар Загребский, 19/1, Санкт-Петербург, 192284; Петербургское шоссе, 2, Пушкин, Санкт-Петербург, 196601

Andrey Valeryevich Dubrovin - Candidate of Veterinary Sciences, Biotechnologist of the Molecular Genetic Laboratory; Candidate of Veterinary Sciences, Senior Researcher at the Department of Large Animal Husbandry.

19/1 Zagrebskiy Аve., Saint Petersburg, 1192284; Peterburgskoe Highway, Pushkin, St. Petersburg, 196601

dubrowin.a.v@yandex.ruhttps://orcid.org/0000-0001-9001-2432ТюринаД. Г.TurinaD. G.

Дарья Георгиевна Тюрина - кандидат экономических наук, главный биотехнолог молекулярно-генетической лаборатории.

Бульвар Загребский, 19/1, Санкт-Петербург, 192284

Daria Georgievna Tyurina - Candidate of Economic Sciences, Chief Biotechnologist of the Molecular Genetic Laboratory.

19/1 Zagrebskiy Аve., Saint Petersburg, 1192284

tiurina@biotrof.ruhttps://orcid.org/0000-0002-9541-6839СоколоваК. А.SokolovaK. A.

Ксения Андреевна Соколова - биотехнолог молекулярно-генетической лаборатории; ассистент кафедры крупного животноводства.

Бульвар Загребский, 19/1, Санкт-Петербург, 192284; Петербургское шоссе, 2, Пушкин, Санкт-Петербург, 196601

Ksenia Andreevna Sokolova - Biotechnologist of the Molecular Genetic Laboratory; Assistant of the Department of Large animal Husbandry.

19/1 Zagrebskiy Аve., Saint Petersburg, 1192284; Peterburgskoe Highway, Pushkin, St. Petersburg, 196601

ksenya.k.a@biotrof.ruhttps://orcid.org/0009-0006-8029-9955ЗаикинВ. А.ZaikinV. A.

Василий Александрович Заикин - биотехнолог молекулярно-генетической лаборатории.

Бульвар Загребский, 19/1, Санкт-Петербург, 192284

Vasily Alexandrovich Zaikin - Biotechnologist of the Molecular Genetic Laboratory.

19/1 Zagrebskiy Аve., Saint Petersburg, 1192284

dfxt@biotrof.ruhttps://orcid.org/0000-0002-4336-8273ПономареваЕ. С.PonomarevaE. S.

Екатерина Сергеевна Пономарева - биотехнолог молекулярно-генетической лаборатории.

Бульвар Загребский, 19/1, Санкт-Петербург, 192284

Ekaterina Sergeevna Ponomareva - Biotechnologist of the Molecular Genetic Laboratory.

19/1 Zagrebskiy Аve., Saint Petersburg, 1192284

kate@biotrof.ruhttps://orcid.org/0009-0008-6484-1235КлючниковаИ. А.KlyuchnikovaI. A.

Ирина Александровна Ключникова - биотехнолог молекулярно-генетической лаборатории1; аспирант.

Бульвар Загребский, 19/1, Санкт-Петербург, 192284; Петербургское шоссе, 2, Пушкин, Санкт-Петербург, 196601

Irina Alexandrovna Klyuchnikova - Biotechnologist of the Molecular Genetic Laboratory; Graduate Student.

19/1 Zagrebskiy Аve., Saint Petersburg, 1192284; Peterburgskoe Highway, Pushkin, St. Petersburg, 196601

irina@biotrof.ruhttps://orcid.org/0000-0003-0081-6336ФисининВ. И.FisininV. I.

Владимир Иванович Фисинин - доктор сельскохозяйственных наук, профессор, академик РАН, научный руководитель.

Ул. Птицеградская, 10, Сергиев Посад, Московская обл., 141311

Vladimir Ivanovich Fisinin - Doctor of Agricultural Sciences, Professor, Academician of the Russian Academy of Sciences, Scientific Supervisor.

10 Ptitsegradskaya Str., Sergiev Posad, Moscow region, 141311

https://orcid.org/0000-0001-9122-9553ЕгоровИ. А.ЕgorovI. A.

Иван Афанасьевич Егоров - доктор биологических наук, профессор, академик РАН.

Ул. Птицеградская, 10, Сергиев Посад, Московская обл., 141311

Ivan Afanasievich Egorov - Doctor of Biological Sciences, Professor, Academician of the Russian Academy of Sciences.

10 Ptitsegradskaya Str., Sergiev Posad, Moscow region, 141311

https://orcid.org/0000-0002-5102-2248ЕгороваТ. А.ЕgorovaT. A.

Татьяна Анатольевна Егорова - доктор сельскохозяйственных наук, заместитель директора по научно-исследовательской работе.

Ул. Птицеградская, 10, Сергиев Посад, Московская обл., 141311

Tatyana Anatolyevna Egorova - Doctor of Agricultural Sciences, Deputy Director for Research.

10 Ptitsegradskaya Str., Sergiev Posad, Moscow region, 141311

eta164@yandex.ruhttps://orcid.org/0000-0003-4564-4427МанукянВ. А.ManukyanV. A.

Вардгес Агавардович Манукян - доктор сельскохозяйственных наук, главный научный сотрудник, заведующий отделом питания птицы.

Ул. Птицеградская, 10, Сергиев Посад, Московская обл., 141311

Vardges Aghavardovich Manukyan - Doctor of Agricultural Sciences, Chief Researcher, Head of the Poultry Nutrition Department.

10 Ptitsegradskaya Str., Sergiev Posad, Moscow region, 141311

manukyan@vnitip.ruhttps://orcid.org/0000-0001-8391-5000ЛенковаТ. Н.LenkovaT. N.

Татьяна Николаевна Ленкова - доктор сельскохозяйственных наук, профессор.

Ул. Птицеградская, 10, Сергиев Посад, Московская обл., 141311

Tatyana Nikolaevna Lenkova - Doctor of Agricultural Sciences, Professor.

10 Ptitsegradskaya Str., Sergiev Posad, Moscow region, 141311

https://orcid.org/0000-0001-7243-7381ДегтяреваО. Н.DegtyarevaO. N.

Ольга Николаевна Дегтярева - кандидат сельскохозяйственных наук, научный сотрудник.

Ул. Птицеградская, 10, Сергиев Посад, Московская обл., 141311

Olga Nikolaevna Degtyareva - Candidate of Agricultural Sciences, Researcher.

10 Ptitsegradskaya Str., Sergiev Posad, Moscow region, 141311

fncvnitip@mail.ruООО «БИОТРОФ+»; Санкт-Петербургский государственный аграрный университетРоссия«BIOTROF+» Ltd; Saint Petersburg State Agrarian UniversityRussian FederationООО «БИОТРОФ+»Россия«BIOTROF+» LtdRussian FederationВсероссийский научно-исследовательский и технологический институт птицеводстваРоссияAll-Russian Scientific Research and Technological Institute of Poultry FarmingRussian Federation202518022025028794Copyright © Йылдырым Е.А., Ильина Л.А., Лаптев Г.Ю., Филиппова В.А., Дубровин А.В., Тюрина Д.Г., Соколова К.А., Заикин В.А., Пономарева Е.С., Ключникова И.А., Фисинин В.И., Егоров И.А., Егорова Т.А., Манукян В.А., Ленкова Т.Н., Дегтярева О.Н., 20252025Йылдырым Е.А., Ильина Л.А., Лаптев Г.Ю., Филиппова В.А., Дубровин А.В., Тюрина Д.Г., Соколова К.А., Заикин В.А., Пономарева Е.С., Ключникова И.А., Фисинин В.И., Егоров И.А., Егорова Т.А., Манукян В.А., Ленкова Т.Н., Дегтярева О.Н.Yildirim E.A., Ilyina L.A., Laptev G.Y., Filippova V.A., Dubrovin A.V., Turina D.G., Sokolova K.A., Zaikin V.A., Ponomareva E.S., Klyuchnikova I.A., Fisinin V.I., Еgorov I.A., Еgorova T.A., Manukyan V.A., Lenkova T.N., Degtyareva O.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vetpress.ru/jour/article/view/3450

Цель исследования — оценить влияние комбикормов с пониженной (на 5%) питательностью по лизину, метионину и обменной энергии при включении в них лизина и метионина в различных формах при их взаимодействии с полом бройлеров на профиль транскрипции ключевых генов, регулирующих антиоксидантную защиту, иммунную систему, воспаление и апоптоз, продуктивность и барьерную функцию эпителия ЖКТ.

Методы

Методы. Эксперимент проводили в СГЦ «Загорское ЭПХ» в 2024 г. на мясной птице кросса «Смена 9» с cуточного до 35-суточного возраста. Состав рациона контрольной группы I включал основной рацион (ОР) с применением монохлоргидрата лизина и DL-метионина, опытной II — ОР с применением сульфата лизина и гидроксианалога метионина, опытной III — ОР с пониженными (на 5%) уровнями лизина в форме монохлоргидрата, DL-метионина и обменной энергии, опытной IV — ОР с пониженными (на 5%) уровнями сульфата лизина, гидроксианалога метионина и обменной энергии. Анализ экспрессии генов проводили с помощью.Real-time.PCR.с обратной транскрипцией.

Результаты

Результаты. Живая масса петушков и курочек в опытных группах II и IV была несколько выше по сравнению с контролем I, тогда как в группе III — ниже. Изменение состава рациона у петушков и курочек оказало во многих случаях значительное влияние на экспрессию ряда ключевых генов. У петушков экспрессия PTGS2 в опытных группах II–IV резко возрастала (от 4,9 до 52,0 раз) по сравнению с контролем I, тогда как у курочек возрастала лишь в 1,5–2,3 раза. Экспрессия мРНК гена Muc2 у курочек снижалась в опытной группе II в 1,9 раза по сравнению с группой I, тогда как у петушков, напротив, повышалась в 3,1 раза.

The

The. aim of the. study was to evaluate the effect of compound feeds with reduced (by 5%) nutritional content of lysine, methionine and metabolic energy when lysine and methionine are included in them in various forms during their interaction with broiler sex on the transcription profile of key genes regulating antioxidant protection, the immune system, inflammation and apoptosis, productivity and barrier function of the gastrointestinal epithelium.

Methods

Methods. The experiment was carried out at the “Zagorskoye” in 2024 on a poultry meat of the “Smena 9” cross from 1 to 35 days of age.

Results

Results. The live weight of rooster and hens in experimental groups II and IV was slightly higher compared to control I, whereas in group III it was lower. In many cases, changes in the composition of the diet of rooster and hens had a significant impact on the expression of a number of key genes. In roosters, PTGS2 expression in experimental groups II–IV increased sharply (from 4.9 to 52.0 times) compared with control I, whereas in hens it increased only 1.5–2.3 times. The expression of the Muc2 gene mRNA in hens decreased in experimental group II by 1.9 times compared with group I, whereas in roosters, on the contrary, it increased by 3.1 times.

цыплята-бройлерыпитательные веществааминокислотылизинметионинэкспрессия геновколичественная ПЦРbroiler chickensnutrientsamino acidslysinemethioninegene expressionquantitative PCRИсследование выполнено за счет средств гранта Российского научного фонда № 22-66-00061 «Экспрессия генов продуктивности и резистентности кур нового отечественного кросса “Смена 9” и ее влияние на иммунитет, особенности реализации генетического потенциала продуктивности при разном энергоаминокислотном питании»The study was funded by the grant of the Russian Science Foundation No. 22-66-00061 «Expression of productivity and resistance genes in chickens of the new domestic cross “Smena 9” and its effect on immunity, features of the realization of the genetic potential of productivity with different energy-amino acid nutrition»ReferencesMuharlien, Nursita I.W., Pangestu V.M. The Effect of Feed Protein Level on Feed Consumption, Body Weight Gain and Feed Conversion of Finisher Java Super Male Chicken. IOP Conference Series: Earth and Environmental Science.2020; 478: 012044. https://doi.org/10.1088/1755-1315/478/1/012044Muharlien, Nursita I.W., Pangestu V.M. The Effect of Feed Protein Level on Feed Consumption, Body Weight Gain and Feed Conversion of Finisher Java Super Male Chicken. IOP Conference Series: Earth and Environmental Science.2020; 478: 012044. https://doi.org/10.1088/1755-1315/478/1/012044Pakiding W., Hakim M.R., Daryatmo, Linggi T.R., Elis. The influence of protein levels on body weight, body dimensions, and reproductive characteristics of local chickens treated in-ovo feeding L-Arginine for two generations. IOP Conference Series: Earth and Environmental Science.2021; 788: 012188. https://doi.org/10.1088/1755-1315/788/1/012188Pakiding W., Hakim M.R., Daryatmo, Linggi T.R., Elis. The influence of protein levels on body weight, body dimensions, and reproductive characteristics of local chickens treated in-ovo feeding L-Arginine for two generations. IOP Conference Series: Earth and Environmental Science.2021; 788: 012188. https://doi.org/10.1088/1755-1315/788/1/012188Houshmand M., Azhar K., Zulkifli I., Bejo M.H., Kamyab A. Effects of non-antibiotic feed additives on performance, immunity and intestinal morphology of broilers fed different levels of protein. South African Journal of Animal Science. 2012; 42(1): 22–32. https://doi.org/10.4314/sajas.v42i1.3Houshmand M., Azhar K., Zulkifli I., Bejo M.H., Kamyab A. Effects of non-antibiotic feed additives on performance, immunity and intestinal morphology of broilers fed different levels of protein. South African Journal of Animal Science. 2012; 42(1): 22–32. https://doi.org/10.4314/sajas.v42i1.3Hernández F., López M., Martínez S., Megías M.D., Catalá P., Madrid J. Effect of low-protein diets and single sex on production performance, plasma metabolites, digestibility, and nitrogen excretion in 1to 48-day-old broilers. Poultry Science. 2012; 91(3): 683–692. https://doi.org/10.3382/ps.2011-01735Hernández F., López M., Martínez S., Megías M.D., Catalá P., Madrid J. Effect of low-protein diets and single sex on production performance, plasma metabolites, digestibility, and nitrogen excretion in 1to 48-day-old broilers. Poultry Science. 2012; 91(3): 683–692. https://doi.org/10.3382/ps.2011-01735Awad E.A. et al. Response of broiler to reduced-protein diets under heat stress conditions. World’s.Poultry Science Journal.2019; 75(4): 583–598. https://doi.org/10.1017/S0043933919000576Awad E.A. et al. Response of broiler to reduced-protein diets under heat stress conditions. World’s.Poultry Science Journal.2019; 75(4): 583–598. https://doi.org/10.1017/S0043933919000576Kidd M.T., McDaniel C.D., Branton S.L., Miller E.R., Boren B.B., Fancher B.I. Increasing Amino Acid Density Improves Live Performance and Carcass Yields of Commercial Broilers. Journal of Applied Poultry Research. 2004; 13(4): 593–604. https://doi.org/10.1093/japr/13.4.593Kidd M.T., McDaniel C.D., Branton S.L., Miller E.R., Boren B.B., Fancher B.I. Increasing Amino Acid Density Improves Live Performance and Carcass Yields of Commercial Broilers. Journal of Applied Poultry Research. 2004; 13(4): 593–604. https://doi.org/10.1093/japr/13.4.593Waldroup P.W., Oviedo-Rondon E.O. Models to Estimate Amino Acid Requirements for Broiler Chickens: A Review International Journal of Poultry Science. 2002; 1(5): 106–113. https://doi.org/10.3923/ijps.2002.106.113Waldroup P.W., Oviedo-Rondon E.O. Models to Estimate Amino Acid Requirements for Broiler Chickens: A Review International Journal of Poultry Science. 2002; 1(5): 106–113. https://doi.org/10.3923/ijps.2002.106.113Smiricky-Tjardes M.R., Mavromichalis I., Albin D.M., Wubben J.E., Rademacher M., Gabert V.M. Bioefficacy of L-lysine sulfate compared with feed-grade L-lysine•HCl in young pigs. Journal of animal science. 2004; 82(9): 2610–2614. https://doi.org/10.2527/2004.8292610xSmiricky-Tjardes M.R., Mavromichalis I., Albin D.M., Wubben J.E., Rademacher M., Gabert V.M. Bioefficacy of L-lysine sulfate compared with feed-grade L-lysine•HCl in young pigs. Journal of animal science. 2004; 82(9): 2610–2614. https://doi.org/10.2527/2004.8292610xAhmad G., Mushtaq T., Aslam Mirza M., Ahmed Z. Comparative Bioefficacy of Lysine from l-Lysine Hydrochloride or l-Lysine Sulfate in Basal Diets Containing Graded Levels of Canola Meal for Female Broiler Chickens. Poultry Science. 2007; 86(3): 525–530. https://doi.org/10.1093/ps/86.3.525Ahmad G., Mushtaq T., Aslam Mirza M., Ahmed Z. Comparative Bioefficacy of Lysine from l-Lysine Hydrochloride or l-Lysine Sulfate in Basal Diets Containing Graded Levels of Canola Meal for Female Broiler Chickens. Poultry Science. 2007; 86(3): 525–530. https://doi.org/10.1093/ps/86.3.525Sauer N., Emrich K., Piepho H.-P., Lemme A., Redshaw M.S., Mosenthin R. Meta-Analysis of the Relative Efficiency of Methionine-Hydroxy-Analogue-Free-Acid Compared with dl-Methionine in Broilers Using Nonlinear Mixed Models. Poultry Science. 2008; 87(10): 2023–2031. https://doi.org/10.3382/ps.2007-00514Sauer N., Emrich K., Piepho H.-P., Lemme A., Redshaw M.S., Mosenthin R. Meta-Analysis of the Relative Efficiency of Methionine-Hydroxy-Analogue-Free-Acid Compared with dl-Methionine in Broilers Using Nonlinear Mixed Models. Poultry Science. 2008; 87(10): 2023–2031. https://doi.org/10.3382/ps.2007-00514Kluge H., Gessner D.K., Herzog E., Eder K. Efficacy of DL-methionine hydroxy analogue-free acid in comparison to DL-methionine in growing male white Pekin ducks. Poultry Science. 2016; 95(3): 590–594. https://doi.org/10.3382/ps/pev355Kluge H., Gessner D.K., Herzog E., Eder K. Efficacy of DL-methionine hydroxy analogue-free acid in comparison to DL-methionine in growing male white Pekin ducks. Poultry Science. 2016; 95(3): 590–594. https://doi.org/10.3382/ps/pev355Martín-Venegas R., Geraert P.A., Ferrer R. Conversion of the Methionine Hydroxy Analogue dl-2-Hydroxy-(4-Methylthio) Butanoic Acid to Sulfur-Containing Amino Acids in the Chicken Small Intestine. Poultry Science. 2006; 85(11): 1932–1938. https://doi.org/10.1093/ps/85.11.1932Martín-Venegas R., Geraert P.A., Ferrer R. Conversion of the Methionine Hydroxy Analogue dl-2-Hydroxy-(4-Methylthio) Butanoic Acid to Sulfur-Containing Amino Acids in the Chicken Small Intestine. Poultry Science. 2006; 85(11): 1932–1938. https://doi.org/10.1093/ps/85.11.1932Zarghi H., Ghavi S. Relative biological efficacy of methionine hydroxy analogue-free acid compared to dl-methionine in the broiler chickens. Veterinary Medicine and Science. 2024; 10(3): e1460. https://doi.org/10.1002/vms3.1460Zarghi H., Ghavi S. Relative biological efficacy of methionine hydroxy analogue-free acid compared to dl-methionine in the broiler chickens. Veterinary Medicine and Science. 2024; 10(3): e1460. https://doi.org/10.1002/vms3.1460Gelli M., Duo Y., Konda A.R., Zhang C., Holding D., Dweikat I. Identification of differentially expressed genes between sorghum genotypes with contrasting nitrogen stress tolerance by genome-wide transcriptional profiling. BMC Genomics. 2014; 15: 179. https://doi.org/10.1186/1471-2164-15-179Gelli M., Duo Y., Konda A.R., Zhang C., Holding D., Dweikat I. Identification of differentially expressed genes between sorghum genotypes with contrasting nitrogen stress tolerance by genome-wide transcriptional profiling. BMC Genomics. 2014; 15: 179. https://doi.org/10.1186/1471-2164-15-179Pauletto M. et al. Nutrigenomic Effects of Long-Term Grape Pomace Supplementation in Dairy Cows. Animals. 2020; 10(4): 714. https://doi.org/10.3390/ani10040714Pauletto M. et al. Nutrigenomic Effects of Long-Term Grape Pomace Supplementation in Dairy Cows. Animals. 2020; 10(4): 714. https://doi.org/10.3390/ani10040714Vázquez-AñÓn M., Kratzer D., González-Esquerra R., Yi I.G., Knight C.D. A Multiple Regression Model Approach to Contrast The Performance of 2-Hydroxy-4-Methylthio Butanoic Acid and DL-Methionine Supplementation Tested in Broiler Experiments and Reported in the Literature. Poultry Science. 2006; 85(4): 693–705. https://doi.org/10.1093/ps/85.4.693Vázquez-AñÓn M., Kratzer D., González-Esquerra R., Yi I.G., Knight C.D. A Multiple Regression Model Approach to Contrast The Performance of 2-Hydroxy-4-Methylthio Butanoic Acid and DL-Methionine Supplementation Tested in Broiler Experiments and Reported in the Literature. Poultry Science. 2006; 85(4): 693–705. https://doi.org/10.1093/ps/85.4.693Surai P.F. Integrated Antioxidant Defence Network in Animals. EC. Nutrition. 2023; 18(6): 18–20.Surai P.F. Integrated Antioxidant Defence Network in Animals. EC. Nutrition. 2023; 18(6): 18–20.Surai F. Vitagenes in avian biology and poultry health. Wageningen, The Netherlands: Wageningen Academic Publishers. 2020; 544. ISBN 978-90-8686-353-2 https://doi.org/10.3920/978-90-8686-906-0Surai F. Vitagenes in avian biology and poultry health. Wageningen, The Netherlands: Wageningen Academic Publishers. 2020; 544. ISBN 978-90-8686-353-2 https://doi.org/10.3920/978-90-8686-906-0Martín-Vázquez E., Cobo-Vuilleumier N., López-Noriega L., Lorenzo P.I., Gauthier B.R. The PTGS2/COX2-PGE signaling cascade in inflammation: Pro or anti? A case study with type 1 diabetes mellitus. International Journal of Biological Sciences. 2023; 19(13): 4157–4165. https://doi.org/10.7150/ijbs.86492Martín-Vázquez E., Cobo-Vuilleumier N., López-Noriega L., Lorenzo P.I., Gauthier B.R. The PTGS2/COX2-PGE signaling cascade in inflammation: Pro or anti? A case study with type 1 diabetes mellitus. International Journal of Biological Sciences. 2023; 19(13): 4157–4165. https://doi.org/10.7150/ijbs.86492Surai P.F., Kochish I.I., Kidd M.T. Redox Homeostasis in Poultry: Regulatory Roles of NF-κB. Antioxidants. 2021; 10(2): 186. https://doi.org/10.3390/antiox10020186Surai P.F., Kochish I.I., Kidd M.T. Redox Homeostasis in Poultry: Regulatory Roles of NF-κB. Antioxidants. 2021; 10(2): 186. https://doi.org/10.3390/antiox10020186Burdick Sanchez N.C., Broadway P.R., Carroll J.A. Influence of Yeast Products on Modulating Metabolism and Immunity in Cattle and Swine. Animals. 2021; 11(2): 371. https://doi.org/10.3390/ani11020371Burdick Sanchez N.C., Broadway P.R., Carroll J.A. Influence of Yeast Products on Modulating Metabolism and Immunity in Cattle and Swine. Animals. 2021; 11(2): 371. https://doi.org/10.3390/ani11020371Kern L., Mittenbühler M.J., Vesting A.J., Ostermann A.L., Wunderlich C.M., Wunderlich F.T. Obesity-Induced TNFα and IL-6 Signaling: The Missing Link between Obesity and Inflammation— Driven Liver and Colorectal Cancers. Cancers. 2019; 11(1): 24. https://doi.org/10.3390/cancers11010024Kern L., Mittenbühler M.J., Vesting A.J., Ostermann A.L., Wunderlich C.M., Wunderlich F.T. Obesity-Induced TNFα and IL-6 Signaling: The Missing Link between Obesity and Inflammation— Driven Liver and Colorectal Cancers. Cancers. 2019; 11(1): 24. https://doi.org/10.3390/cancers11010024Ghezzi C., Loo D.D.F., Wright E.M. Physiology of renal glucose handling via SGLT1, SGLT2 and GLUT2. Diabetologia.2018; 61(10): 2087–2097. https://doi.org/10.1007/s00125-018-4656-5Ghezzi C., Loo D.D.F., Wright E.M. Physiology of renal glucose handling via SGLT1, SGLT2 and GLUT2. Diabetologia.2018; 61(10): 2087–2097. https://doi.org/10.1007/s00125-018-4656-5Weintraut M.L., Kim S., Dalloul R.A., Wong E.A. Expression of small intestinal nutrient transporters in embryonic and posthatch turkeys. Poultry Science. 2016; 95(1): 90–98. https://doi.org/10.3382/ps/pev310Weintraut M.L., Kim S., Dalloul R.A., Wong E.A. Expression of small intestinal nutrient transporters in embryonic and posthatch turkeys. Poultry Science. 2016; 95(1): 90–98. https://doi.org/10.3382/ps/pev310Uni Z., Smirnov A., Sklan D. Preand posthatch development of goblet cells in the broiler small intestine: effect of delayed access to feed. Poultry Science. 2003; 82(2): 320–327. https://doi.org/10.1093/ps/82.2.320Uni Z., Smirnov A., Sklan D. Preand posthatch development of goblet cells in the broiler small intestine: effect of delayed access to feed. Poultry Science. 2003; 82(2): 320–327. https://doi.org/10.1093/ps/82.2.320Horn N.L., Donkin S.S., Applegate T.J., Adeola O. Intestinal mucin dynamics: response of broiler chicks and White Pekin ducklings to dietary threonine. Poultry Science. 2009; 88(9): 1906–1914. https://doi.org/10.3382/ps.2009-00009Horn N.L., Donkin S.S., Applegate T.J., Adeola O. Intestinal mucin dynamics: response of broiler chicks and White Pekin ducklings to dietary threonine. Poultry Science. 2009; 88(9): 1906–1914. https://doi.org/10.3382/ps.2009-00009Celi P., Verlhac V., Pérez Calvo E., Schmeisser J., Kluenter A.-M. Biomarkers of gastrointestinal functionality in animal nutrition and health. Animal Feed Science and Technology. 2019; 250: 9–13. https://doi.org/10.1016/J.ANIFEEDSCI.2018.07.012Celi P., Verlhac V., Pérez Calvo E., Schmeisser J., Kluenter A.-M. Biomarkers of gastrointestinal functionality in animal nutrition and health. Animal Feed Science and Technology. 2019; 250: 9–13. https://doi.org/10.1016/J.ANIFEEDSCI.2018.07.012Faure M., Moënnoz D., Montigon F., Mettraux C., Breuillé D., Ballèvre O. Dietary Threonine Restriction Specifically Reduces Intestinal Mucin Synthesis in Rats The Journal of Nutrition. 2005; 135(3): 486–491. https://doi.org/10.1093/jn/135.3.486Faure M., Moënnoz D., Montigon F., Mettraux C., Breuillé D., Ballèvre O. Dietary Threonine Restriction Specifically Reduces Intestinal Mucin Synthesis in Rats The Journal of Nutrition. 2005; 135(3): 486–491. https://doi.org/10.1093/jn/135.3.486Conde-Aguilera J.A., Cholet J.C.G., Lessire M., Mercier Y., Tesseraud S., van Milgen J. The level and source of free-methionine affect body composition and breast muscle traits in growing broilers. Poultry Science.2016; 95(10): 2322–2331. https://doi.org/10.3382/ps/pew105Conde-Aguilera J.A., Cholet J.C.G., Lessire M., Mercier Y., Tesseraud S., van Milgen J. The level and source of free-methionine affect body composition and breast muscle traits in growing broilers. Poultry Science.2016; 95(10): 2322–2331. https://doi.org/10.3382/ps/pew105

The authors declare that there are no conflicts of interest present.