vetpressАграрная наукаAgrarian science0869-81552686-701XРедакция журнала "Аграрная наука"10.32634/0869-8155-2025-392-03-47-61vetpress-3512Research ArticleЗООТЕХНИЯZOOTECHNICSДинамика экстракции минералов из кормового субстрата in vitro при внесении в реакционную среду рубцового содержимого низкомолекулярных добавокDynamics of extraction of minerals from the feed substrate in vitro with including low molecular weight additives into rumen reaction mediumhttps://orcid.org/0000-0003-3977-4831АтландероваК. Н.AtlanderovaK. N.

Атландерова Ксения Николаевна - кандидат биологических наук, научный сотрудник.

ул. 9 Января, 29, Оренбург, 460000

Ksenia N. Atlanderova - Candidate of Biological Sciences, Researcher.

29 9th Yanvarya Str., Orenburg, 460000

atlander-kn@mail.ruhttps://orcid.org/0000-0003-3086-681XШошинД. Е.ShoshinD. E.

Шошин Даниил Евгеньевич - младший научный сотрудник.

ул. 9 Января, 29, Оренбург, 460000

Daniil E. Shoshin - Junior Researcher.

29 9th Yanvarya Str., Orenburg, 460000

daniilshoshin@mail.ruhttps://orcid.org/0000-0002-0443-6990КазаевК. А.KazaevK. A.

Казаев Кирилл Александрович - младший научный сотрудник.

ул. 9 Января, 29, Оренбург, 460000

Kirill A. Kazaev - Junior Researcher.

29 9th Yanvarya Str., Orenburg, 460000

kazaevk970@gmail.comФедеральный научный центр биологических систем и агротехнологий РАНРоссияFederal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of SciencesRussian Federation202521032025034761Copyright © Атландерова К.Н., Шошин Д.Е., Казаев К.А., 20252025Атландерова К.Н., Шошин Д.Е., Казаев К.А.Atlanderova K.N., Shoshin D.E., Kazaev K.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vetpress.ru/jour/article/view/3512

Запрет на применение антибиотиков в терапевтических целях побуждает к поиску высокоэффективных альтернатив, одной из которых являются фитобиотики. Однако перед масштабным внедрением их в практику необходимо проводить детальную оценку воздействия на различные аспекты жизнедеятельности.

Цель работы — изучение динамики элементного профиля рубца жвачных in vitro при внесении в корм ванилина, транскоричного альдегида, дигидроксикверцетина и 7-гидроксикумарина. Исследование проводилось на одноквадрупольном масс-спектрометре с индуктивно связанной плазмой Agilent 7900 ICP-MS (Agilent, США). Анализ таких элементов, как Cr, Fe и Zn, осуществляли в гелиевом режиме с использованием столкновительной ячейки. В ходе работы установлено, что ванилин в концентрации от 1,225 × 10-4 до 4,900 × 10-4 моль/л снижает накопление меди, кобальта и свинца, стимулируя извлечение марганца, железа, алюминия и бария. Кверцетин и коричный альдегид, напротив, подавляют экстракцию почти всех исследованных элементов. Кумарин же в большей степени способствует извлечению минералов из целлюлозной матрицы растительных компонентов корма, за исключением меди, кобальта и цинка. При этом лучший эффект обнаруживает комбинация кверцетина и ванилина в концентрациях 2,450 × 10-4 и 1,225 × 10-4 моль/л соответственно. Все изученные вещества демонстрируют потенциал для коррекции гипо- и гиперэлементозов различной направленности.

The ban on the use of antibiotics for therapeutic purposes encourages the search for highly effective alternatives, one of which is phytobiotics. However, before their large-scale implementation into practice, it is necessary to conduct a detailed assessment of the impact on various aspects of life.

The aim of the work was to study the dynamics of the elemental profile of the ruminant rumen in vitro when vanillin, transcoric aldehyde, dihydroxyquercetin and 7-hydroxycoumarin were introduced into feed.

The study was conducted on a single-quadrupole inductively coupled plasma mass spectrometer Agilent 7900 ICP-MS (Agilent, USA). The analysis of elements such as Cr, Fe and Zn was carried out in the helium mode using a collision cell. During the work, it was found that vanillin in concentrations from 1,225 × 10-4 to 4,900 × 10-4 mol/l reduces the accumulation of copper, cobalt and lead, stimulating the extraction of manganese, iron, aluminum and barium. Quercetin and cinnamic aldehyde, on the contrary, inhibit the extraction of almost all the studied elements. Coumarin, on the other hand, contributes more to the extraction of minerals from the cellulose matrix of plant components of the feed, with the exception of copper, cobalt and zinc. At the same time, the best effect is detected by a combination of quercetin and vanillin at concentrations of 2,450 × 10-4 and 1,225 × 10-4 mol/l, respectively. All the studied substances demonstrate the potential for the correction of hypo- and hyperelementoses of various types.

фитобиотикижвачные животныерубецэлементный профилькверцетинкумаринванилинкоричный альдегидphytobioticsruminantsrumenelemental profilequercetincoumarinvanillincinnamon aldehydeReferencesHan D. et al. Application and substitution of antibiotics in animal feeding. Medycyna Weterynaryjna. 2024; 80(1): 5–11. https://doi.org/10.21521/mw.6830Han D. et al. Application and substitution of antibiotics in animal feeding. Medycyna Weterynaryjna. 2024; 80(1): 5–11. https://doi.org/10.21521/mw.6830Meek R.W., Vyas H., Piddock L.J.V. Nonmedical Uses of Antibiotics: Time to Restrict Their Use?. PLoS Biology. 2015; 13(10): e1002266. https://doi.org/10.1371/journal.pbio.1002266Meek R.W., Vyas H., Piddock L.J.V. Nonmedical Uses of Antibiotics: Time to Restrict Their Use?. PLoS Biology. 2015; 13(10): e1002266. https://doi.org/10.1371/journal.pbio.1002266Oliveira N.A., Gonçalves B.L., Lee S.H., Oliveira C.A.F., Corassin C.H. Use of Antibiotics in Animal Production and its Impact on Human Health. Journal of Food Chemistry and Nanotechnology. 2020; 6(1): 40–47. https://doi.org/10.17756/jfcn.2020-082Oliveira N.A., Gonçalves B.L., Lee S.H., Oliveira C.A.F., Corassin C.H. Use of Antibiotics in Animal Production and its Impact on Human Health. Journal of Food Chemistry and Nanotechnology. 2020; 6(1): 40–47. https://doi.org/10.17756/jfcn.2020-082Шошин Д.Е., Сизова Е.А., Камирова А.М. Бактериальная люминесценция марганец- и кобальтсодержащих ультрадисперсных частиц (Mn2O3 и Co3O4) в рубцовой жидкости. Сельскохозяйственная биология. 2023; 58(6): 1122–1136. https://doi.org/10.15389/agrobiology.2023.6.1122rusShoshin D.E., Sizova E.A., Kamirova A.M. Bacterial luminescence of manganese- and cobalt-containing ultrafine particles (Mn2O3 and Co3O4) in the rumen fluid. Agricultural Biology. 2023; 58(6): 1122–1136. https://doi.org/10.15389/agrobiology.2023.6.1122engEbeid T. et al. Impact of probiotics and/or organic acids supplementation on growth performance, microbiota, antioxidative status, and immune response of broilers. Italian Journal of Animal Science. 2021; 20(1): 2263–2273. https://doi.org/10.1080/1828051X.2021.2012092Ebeid T. et al. Impact of probiotics and/or organic acids supplementation on growth performance, microbiota, antioxidative status, and immune response of broilers. Italian Journal of Animal Science. 2021; 20(1): 2263–2273. https://doi.org/10.1080/1828051X.2021.2012092Shoshin D.E., Sizova E.A., Kamirova A.M. Morphological changes and luminescence of Escherichia coli in contact with Mn2O3 and Co3O4 ultrafine particles as components of a mineral feed additive. Veterinary World. 2024; 17(8): 1880–1888. https://doi.org/10.14202/vetworld.2024.1880-1888Shoshin D.E., Sizova E.A., Kamirova A.M. Morphological changes and luminescence of Escherichia coli in contact with Mn2O3 and Co3O4 ultrafine particles as components of a mineral feed additive. Veterinary World. 2024; 17(8): 1880–1888. https://doi.org/10.14202/vetworld.2024.1880-1888Mba I.E., Nweze E.I. Nanoparticles as therapeutic options for treating multidrug-resistant bacteria: research progress, challenges, and prospects. World Journal of Microbiology and Biotechnology. 2021; 37: 108. https://doi.org/10.1007/s11274-021-03070-xMba I.E., Nweze E.I. Nanoparticles as therapeutic options for treating multidrug-resistant bacteria: research progress, challenges, and prospects. World Journal of Microbiology and Biotechnology. 2021; 37: 108. https://doi.org/10.1007/s11274-021-03070-xСизова Е.А., Нечитайло К.С., Лебедев С.В. Фитобиотики как потенциальные регуляторы функциональной активности микробиома кишечника у цыплят-бройлеров (мини-обзор). Сельскохозяйственная биология. 2022; 57(6): 1071–1082. https://doi.org/10.15389/agrobiology.2022.6.1071rusSizova E.A., Nechitailo K.S., Lebedev S.V. Phytobiotics as potential regulators of the gut microbiome composition and functional activity in broiler chickens (а mini-review). Agricultural Biology. 2022; 57(6): 1071–1082. https://doi.org/10.15389/agrobiology.2022.6.1071engUrban J. et al. Enhancing broiler chicken health and performance: the impact of phytobiotics on growth, gut microbiota, antioxidants, and immunity. Phytochemistry Reviews. 2024. https://doi.org/10.1007/s11101-024-09994-0Urban J. et al. Enhancing broiler chicken health and performance: the impact of phytobiotics on growth, gut microbiota, antioxidants, and immunity. Phytochemistry Reviews. 2024. https://doi.org/10.1007/s11101-024-09994-0Шейда Е.В., Рязанов В.А., Дускаев Г.К., Рахматуллин Ш.Г., Кван О.В. Влияние Artemisiae absinthil herba и Inulae rhizomata et radices на процессы ферментации и метаногенез в рубце молодняка крупного рогатого скота. Аграрная наука. 2023; (3): 46–51. https://doi.org/10.32634/0869-8155-2023-368-3-46-51Sheida E.V., Ryazanov V.A., Duskaev G.K., Rakhmatullin Sh.G., Kvan O.V. Influence of Artemisiae absinthil herba and Inulae rhizomata et radices on fermentation processes and methanogenesis in the rumen of young cattle. Agrarian science. 2023; (3): 46–51 (in Russian). https://doi.org/10.32634/0869-8155-2023-368-3-46-51Mohammadi Gheisar M., Kim I.H. Phytobiotics in poultry and swine nutrition (a review). Italian Journal of Animal Science. 2018; 17(1): 92–99. https://doi.org/10.1080/1828051X.2017.1350120Mohammadi Gheisar M., Kim I.H. Phytobiotics in poultry and swine nutrition (a review). Italian Journal of Animal Science. 2018; 17(1): 92–99. https://doi.org/10.1080/1828051X.2017.1350120Багно О.А., Прохоров О.Н., Шевченко С.А., Шевченко А.И., Дядичкина Т.В. Фитобиотики в кормлении сельскохозяйственных животных (обзор). Сельскохозяйственная биология. 2018; 53(4): 687–697. https://doi.org/10.15389/agrobiology.2018.4.687rusBagno O.A., Prokhorov O.N., Shevchenko S.A., Shevchenko A.I., Dyadichkina T.V. Use of phytobioticts in farm animal feeding (review). Agricultural Biology. 2018; 53(4): 687–697. https://doi.org/10.15389/agrobiology.2018.4.687engРязанов В.А., Курилкина М.Я., Дускаев Г.К., Габидулин В.М. Фитобиотики как альтернатива антибиотикам в животноводстве. Животноводство и кормопроизводство. 2021; 104(4): 108–123. https://doi.org/10.33284/2658-3135-104-4-108Ryazanov V.A., Kurilkina M.Ya., Duskaev G.K., Gabidulin V.M. Phytobiotics as an alternative to antibiotics in animal husbandry (review). Animal husbandry and fodder production. 2021; 104(4): 108–123 (in Russian). https://doi.org/10.33284/2658-3135-104-4-108Zaikina A.S., Buryakov N.P., Buryakova M.A., Zagarin A.Yu., Razhev A.A., Aleshin D.E. Impact of Supplementing Phytobiotics as a Substitute for Antibiotics in Broiler Chicken Feed on Growth Performance, Nutrient Digestibility, and Biochemical Parameters. Veterinary Sciences. 2022; 9(12): 672. https://doi.org/10.3390/vetsci9120672Zaikina A.S., Buryakov N.P., Buryakova M.A., Zagarin A.Yu., Razhev A.A., Aleshin D.E. Impact of Supplementing Phytobiotics as a Substitute for Antibiotics in Broiler Chicken Feed on Growth Performance, Nutrient Digestibility, and Biochemical Parameters. Veterinary Sciences. 2022; 9(12): 672. https://doi.org/10.3390/vetsci9120672Atlanderova K., Shoshin D., Kazaev K. Amino acid status and nitrogen forms of rumen contents in vitro when phytogenic components are added to the reaction medium. E3S Web of Conferences. 2024; 548: 02006. https://doi.org/10.1051/e3sconf/202454802006Atlanderova K., Shoshin D., Kazaev K. Amino acid status and nitrogen forms of rumen contents in vitro when phytogenic components are added to the reaction medium. E3S Web of Conferences. 2024; 548: 02006. https://doi.org/10.1051/e3sconf/202454802006Bhalakiya N., Haque N., Patel P., Joshi P. Role of Trace Minerals in Animal Production and Reproduction. International Journal of Livestock Research. 2019; 9(9): 1–12.Bhalakiya N., Haque N., Patel P., Joshi P. Role of Trace Minerals in Animal Production and Reproduction. International Journal of Livestock Research. 2019; 9(9): 1–12.Mousavi Khaneghah A., Fakhri Y., Nematollahi A., Pirhadi M. Potentially toxic elements (PTEs) in cereal-based foods: A systematic review and meta-analysis. Trends in Food Science & Technology. 2020; 96: 30–44. https://doi.org/10.1016/j.tifs.2019.12.007Mousavi Khaneghah A., Fakhri Y., Nematollahi A., Pirhadi M. Potentially toxic elements (PTEs) in cereal-based foods: A systematic review and meta-analysis. Trends in Food Science & Technology. 2020; 96: 30–44. https://doi.org/10.1016/j.tifs.2019.12.007Arya S.S., Rookes J.E., Cahill D.M., Lenka S.K. Vanillin: a review on the therapeutic prospects of a popular flavouring molecule. Advances in Traditional Medicine. 2021; 21(3): 415–432. https://doi.org/10.1007/s13596-020-00531-wArya S.S., Rookes J.E., Cahill D.M., Lenka S.K. Vanillin: a review on the therapeutic prospects of a popular flavouring molecule. Advances in Traditional Medicine. 2021; 21(3): 415–432. https://doi.org/10.1007/s13596-020-00531-wSalehi B. et al. Therapeutic Potential of Quercetin: New Insights and Perspectives for Human Health. ACS Omega. 2020; 5(20): 11849–11872. https://doi.org/10.1021/acsomega.0c01818Salehi B. et al. Therapeutic Potential of Quercetin: New Insights and Perspectives for Human Health. ACS Omega. 2020; 5(20): 11849–11872. https://doi.org/10.1021/acsomega.0c01818Błaszczyk N., Rosiak A., Kałużna-Czaplińska J. The Potential Role of Cinnamon in Human Health. Forests. 2021; 12(5): 648. https://doi.org/10.3390/f12050648Błaszczyk N., Rosiak A., Kałużna-Czaplińska J. The Potential Role of Cinnamon in Human Health. Forests. 2021; 12(5): 648. https://doi.org/10.3390/f12050648Garg S.S., Gupta J., Sharma S., Sahu D. An insight into the therapeutic applications of coumarin compounds and their mechanisms of action. European Journal of Pharmaceutical Sciences. 2020; 152: 105424. https://doi.org/10.1016/j.ejps.2020.105424Garg S.S., Gupta J., Sharma S., Sahu D. An insight into the therapeutic applications of coumarin compounds and their mechanisms of action. European Journal of Pharmaceutical Sciences. 2020; 152: 105424. https://doi.org/10.1016/j.ejps.2020.105424Шошин Д.Е., Атландерова К.Н., Дускаев Г.К., Сизова Е.А. Малые молекулы в тесте ингибирования бактериальной люминесценции. Siberian Journal of Life Sciences and Agriculture. 2023; 15(4): 29–55. https://doi.org/10.12731/2658-6649-2023-15-4-29-55Shoshin D.E., Atlanderova K.N., Duskaev G.K., Sizova E.A. Small molecules in the bacterial luminescence inhibition test. Siberian Journal of Life Sciences and Agriculture. 2023; 15(4): 29–55 (in Russian). https://doi.org/10.12731/2658-6649-2023-15-4-29-55Власенко Л.В., Атландерова К.Н., Дускаев Г.К., Шошин Д.Е. Влияние фитохимических веществ на сигнальные молекулы системы Quorum Sensing у бактерий. Международный вестник ветеринарии. 2023; (2): 25–31. https://doi.org/10.52419/issn2072-2419.2023.2.25Vlasenko L.V., Atlanderova K.N., Duskaev G.K., Shoshin D.E. The effect of phytochemicals on the signaling molecules of the “Quorum Sensing” system in bacteria. International Bulletin of Veterinary Medicine. 2023; (2): 25–31 (in Russian). https://doi.org/10.52419/issn2072-2419.2023.2.25Hua D., Hendriks W.H., Xiong B., Pellikaan W.F. Starch and Cellulose Degradation in the Rumen and Applications of Metagenomics on Ruminal Microorganisms. Animals. 2022; 12(21): 3020. https://doi.org/10.3390/ani12213020Hua D., Hendriks W.H., Xiong B., Pellikaan W.F. Starch and Cellulose Degradation in the Rumen and Applications of Metagenomics on Ruminal Microorganisms. Animals. 2022; 12(21): 3020. https://doi.org/10.3390/ani12213020Fraga M., Fernández S., Perelmuter K., Pomiés N., Cajarville C., Zunino P. The use of Prevotella bryantii 3C5 for modulation of the ruminal environment in an ovine model. Brazilian Journal of Microbiology. 2018; 49(S1): 101–106. https://doi.org/10.1016/j.bjm.2018.07.004Fraga M., Fernández S., Perelmuter K., Pomiés N., Cajarville C., Zunino P. The use of Prevotella bryantii 3C5 for modulation of the ruminal environment in an ovine model. Brazilian Journal of Microbiology. 2018; 49(S1): 101–106. https://doi.org/10.1016/j.bjm.2018.07.004Rodríguez Hernáez J. et al. The first complete genomic structure of Butyrivibrio fibrisolvens and its chromid. Microbial Genomics. 2018; 4(10): e000216. https://doi.org/10.1099/mgen.0.000216Rodríguez Hernáez J. et al. The first complete genomic structure of Butyrivibrio fibrisolvens and its chromid. Microbial Genomics. 2018; 4(10): e000216. https://doi.org/10.1099/mgen.0.000216Ward B.K., Dufault R.J., Hassell R., Cutulle M.A. Upscaled Bioammonium/Ammonia Production by Clostridium Aminophilum Cultured with Soy Protein Isolate. Journal of Agricultural and Food Chemistry. 2017; 65(14): 2930–2935. https://doi.org/10.1021/acs.jafc.7b00113Ward B.K., Dufault R.J., Hassell R., Cutulle M.A. Upscaled Bioammonium/Ammonia Production by Clostridium Aminophilum Cultured with Soy Protein Isolate. Journal of Agricultural and Food Chemistry. 2017; 65(14): 2930–2935. https://doi.org/10.1021/acs.jafc.7b00113Liu Y., Kong D., Wu H.-L., Ling H.-Q. Iron in plant–pathogen interactions. Journal of Experimental Botany. 2021; 72(6): 2114–2124. https://doi.org/10.1093/jxb/eraa516Liu Y., Kong D., Wu H.-L., Ling H.-Q. Iron in plant–pathogen interactions. Journal of Experimental Botany. 2021; 72(6): 2114–2124. https://doi.org/10.1093/jxb/eraa516Hilal E.Y., Elkhairey M.A.E., Osman A.O.A. The Role of Zinc, Manganse and Copper in Rumen Metabolism and Immune Function: A Review Article. Open Journal of Animal Sciences. 2016; 6(4): 304–324. https://doi.org/10.4236/ojas.2016.64035Hilal E.Y., Elkhairey M.A.E., Osman A.O.A. The Role of Zinc, Manganse and Copper in Rumen Metabolism and Immune Function: A Review Article. Open Journal of Animal Sciences. 2016; 6(4): 304–324. https://doi.org/10.4236/ojas.2016.64035Schlattl M., Buffler M., Windisch W. Clay Minerals Affect the Solubility of Zn and Other Bivalent Cations in the Digestive Tract of Ruminants In Vitro. Animals. 2021; 11(3): 877. https://doi.org/10.3390/ani11030877Schlattl M., Buffler M., Windisch W. Clay Minerals Affect the Solubility of Zn and Other Bivalent Cations in the Digestive Tract of Ruminants In Vitro. Animals. 2021; 11(3): 877. https://doi.org/10.3390/ani11030877Tsang T., Davis C.I., Brady D.C. Copper biology. Current Biology. 2021; 31(9): R421–R427. https://doi.org/10.1016/j.cub.2021.03.054Tsang T., Davis C.I., Brady D.C. Copper biology. Current Biology. 2021; 31(9): R421–R427. https://doi.org/10.1016/j.cub.2021.03.054De Baaij J.H.F., Hoenderop J.G.J., Bindels R.J.M. Magnesium in Man: Implications for Health and Disease. Physiological Reviews. 2015; 95(1): 1–46. https://doi.org/10.1152/physrev.00012.2014De Baaij J.H.F., Hoenderop J.G.J., Bindels R.J.M. Magnesium in Man: Implications for Health and Disease. Physiological Reviews. 2015; 95(1): 1–46. https://doi.org/10.1152/physrev.00012.2014Palmer B.F., Clegg D.J. Physiology and pathophysiology of potassium homeostasis. Advances in Physiology Education. 2016; 40(4): 480–490. https://doi.org/10.1152/advan.00121.2016Palmer B.F., Clegg D.J. Physiology and pathophysiology of potassium homeostasis. Advances in Physiology Education. 2016; 40(4): 480–490. https://doi.org/10.1152/advan.00121.2016Hu X., Wei X., Ling J., Chen J. Cobalt: An Essential Micronutrient for Plant Growth?. Frontiers in Plant Science. 2021; 12: 768523. https://doi.org/10.3389/fpls.2021.768523Hu X., Wei X., Ling J., Chen J. Cobalt: An Essential Micronutrient for Plant Growth?. Frontiers in Plant Science. 2021; 12: 768523. https://doi.org/10.3389/fpls.2021.768523Alvino L., Pacheco-Herrero M., López-Lorente Á.I., Quiñones Z., Cárdenas S., González-Sánchez Z.I. Toxicity evaluation of barium ferrite nanoparticles in bacteria, yeast and nematode. Chemosphere. 2020; 254: 126786. https://doi.org/10.1016/j.chemosphere.2020.126786Alvino L., Pacheco-Herrero M., López-Lorente Á.I., Quiñones Z., Cárdenas S., González-Sánchez Z.I. Toxicity evaluation of barium ferrite nanoparticles in bacteria, yeast and nematode. Chemosphere. 2020; 254: 126786. https://doi.org/10.1016/j.chemosphere.2020.126786Assi M.A., Hezmee M.N.M., Haron A.W., Sabri M.Y.M., Rajion M.A. The detrimental effects of lead on human and animal health. Veterinary World. 2016; 9(6): 660–671. https://doi.org/10.14202/vetworld.2016.660-671Assi M.A., Hezmee M.N.M., Haron A.W., Sabri M.Y.M., Rajion M.A. The detrimental effects of lead on human and animal health. Veterinary World. 2016; 9(6): 660–671. https://doi.org/10.14202/vetworld.2016.660-671Tahir I., Alkheraije K.A. A review of important heavy metals toxicity with special emphasis on nephrotoxicity and its management in cattle. Frontiers in Veterinary Science. 2023; 10: 1149720. https://doi.org/10.3389/fvets.2023.1149720Tahir I., Alkheraije K.A. A review of important heavy metals toxicity with special emphasis on nephrotoxicity and its management in cattle. Frontiers in Veterinary Science. 2023; 10: 1149720. https://doi.org/10.3389/fvets.2023.1149720Mezzaroba L., Alfieri D.F., Simão A.N.C., Reiche E.M.V. The role of zinc, copper, manganese and iron in neurodegenerative diseases. NeuroToxicology. 2019; 74: 230–241. https://doi.org/10.1016/j.neuro.2019.07.007Mezzaroba L., Alfieri D.F., Simão A.N.C., Reiche E.M.V. The role of zinc, copper, manganese and iron in neurodegenerative diseases. NeuroToxicology. 2019; 74: 230–241. https://doi.org/10.1016/j.neuro.2019.07.007Li L., Yang X. The Essential Element Manganese, Oxidative Stress, and Metabolic Diseases: Links and Interactions. Oxidative Medicine and Cellular Longevity. 2018; 1: 7580707. https://doi.org/10.1155/2018/7580707Li L., Yang X. The Essential Element Manganese, Oxidative Stress, and Metabolic Diseases: Links and Interactions. Oxidative Medicine and Cellular Longevity. 2018; 1: 7580707. https://doi.org/10.1155/2018/7580707Bosma E.F., Rau M.H., van Gijtenbeek L.A., Siedler S. Regulation and distinct physiological roles of manganese in bacteria. FEMS Microbiology Reviews. 2021; 45(6): fuab028. https://doi.org/10.1093/femsre/fuab028Bosma E.F., Rau M.H., van Gijtenbeek L.A., Siedler S. Regulation and distinct physiological roles of manganese in bacteria. FEMS Microbiology Reviews. 2021; 45(6): fuab028. https://doi.org/10.1093/femsre/fuab028Ellis J.L. et al. The effect of lactic acid bacteria included as a probiotic or silage inoculant on in vitro rumen digestibility, total gas and methane production. Animal Feed Science and Technology. 2016; 211: 61–74. https://doi.org/10.1016/j.anifeedsci.2015.10.016Ellis J.L. et al. The effect of lactic acid bacteria included as a probiotic or silage inoculant on in vitro rumen digestibility, total gas and methane production. Animal Feed Science and Technology. 2016; 211: 61–74. https://doi.org/10.1016/j.anifeedsci.2015.10.016Vigh A., Criste A., Gragnic K., Moquet L., Gerard C. Ruminal Solubility and Bioavailability of Inorganic Trace Mineral Sources and Effects on Fermentation Activity Measured in Vitro. Agriculture. 2023; 13(4): 879. https://doi.org/10.3390/agriculture13040879Vigh A., Criste A., Gragnic K., Moquet L., Gerard C. Ruminal Solubility and Bioavailability of Inorganic Trace Mineral Sources and Effects on Fermentation Activity Measured in Vitro. Agriculture. 2023; 13(4): 879. https://doi.org/10.3390/agriculture13040879Baj J. et al. Consequences of Disturbing Manganese Homeostasis. International Journal of Molecular Sciences. 2023; 24(19): 14959. https://doi.org/10.3390/ijms241914959Baj J. et al. Consequences of Disturbing Manganese Homeostasis. International Journal of Molecular Sciences. 2023; 24(19): 14959. https://doi.org/10.3390/ijms241914959Wysocka D., Snarska A., Sobiech P. Iron in cattle health. Journal of Elementology. 2020; 25(3): 1175–1185. https://doi.org/10.5601/jelem.2020.25.2.1960Wysocka D., Snarska A., Sobiech P. Iron in cattle health. Journal of Elementology. 2020; 25(3): 1175–1185. https://doi.org/10.5601/jelem.2020.25.2.1960Puig S., Ramos-Alonso L., Romero A.M., Martínez-Pastor M.T. The elemental role of iron in DNA synthesis and repair. Metallomics. 2017; 9(11): 1483–1500. https://doi.org/10.1039/c7mt00116aPuig S., Ramos-Alonso L., Romero A.M., Martínez-Pastor M.T. The elemental role of iron in DNA synthesis and repair. Metallomics. 2017; 9(11): 1483–1500. https://doi.org/10.1039/c7mt00116aDíaz-Tocados J.M. et al. Effect of a calcium-rich diet on mineral and bone metabolism in rats. Revista de Osteoporosis y Metabolismo Mineral. 2022; 14(1): 48–54. https://doi.org/10.4321/S1889-836X2022000100006Díaz-Tocados J.M. et al. Effect of a calcium-rich diet on mineral and bone metabolism in rats. Revista de Osteoporosis y Metabolismo Mineral. 2022; 14(1): 48–54. https://doi.org/10.4321/S1889-836X2022000100006Wilkens M.R., Nelson C.D., Hernandez L.L., McArt J.A.A. Symposium review: Transition cow calcium homeostasis — Health effects of hypocalcemia and strategies for prevention. Journal of Dairy Science. 2020; 103(3): 2909–2927. https://doi.org/10.3168/jds.2019-17268Wilkens M.R., Nelson C.D., Hernandez L.L., McArt J.A.A. Symposium review: Transition cow calcium homeostasis — Health effects of hypocalcemia and strategies for prevention. Journal of Dairy Science. 2020; 103(3): 2909–2927. https://doi.org/10.3168/jds.2019-17268Matikainen N., Pekkarinen T., Ryhänen E.M., Schalin-Jäntti C. Physiology of Calcium Homeostasis: An Overview. Endocrinology and Metabolism Clinics of North America. 2021; 50(4): 575–590. https://doi.org/10.1016/j.ecl.2021.07.005Matikainen N., Pekkarinen T., Ryhänen E.M., Schalin-Jäntti C. Physiology of Calcium Homeostasis: An Overview. Endocrinology and Metabolism Clinics of North America. 2021; 50(4): 575–590. https://doi.org/10.1016/j.ecl.2021.07.005Al-Fartusie F.S., Mohssan S.N. Essential Trace Elements and Their Vital Roles in Human Body. Indian Journal of Advances in Chemical Science. 2017; 5(3): 127–136.Al-Fartusie F.S., Mohssan S.N. Essential Trace Elements and Their Vital Roles in Human Body. Indian Journal of Advances in Chemical Science. 2017; 5(3): 127–136.Alfano M., Cavazza C. Structure, function, and biosynthesis of nickel‐dependent enzymes. Protein Science. 2020; 29(5): 1071–1089. https://doi.org/10.1002/pro.3836Alfano M., Cavazza C. Structure, function, and biosynthesis of nickel‐dependent enzymes. Protein Science. 2020; 29(5): 1071–1089. https://doi.org/10.1002/pro.3836Wang Y., Jiang M., Zhang Z., Sun H. Effects of over-load iron on nutrient digestibility, haemato-biochemistry, rumen fermentation and bacterial communities in sheep. Journal of Animal Physiology and Animal Nutrition. 2020; 104(1): 32–43. https://doi.org/10.1111/jpn.13225Wang Y., Jiang M., Zhang Z., Sun H. Effects of over-load iron on nutrient digestibility, haemato-biochemistry, rumen fermentation and bacterial communities in sheep. Journal of Animal Physiology and Animal Nutrition. 2020; 104(1): 32–43. https://doi.org/10.1111/jpn.13225Nguyen T.L.A., Bhattacharya D. Antimicrobial Activity of Quercetin: An Approach to Its Mechanistic Principle. Molecules. 2022; 27(8): 2494. https://doi.org/10.3390/molecules27082494Nguyen T.L.A., Bhattacharya D. Antimicrobial Activity of Quercetin: An Approach to Its Mechanistic Principle. Molecules. 2022; 27(8): 2494. https://doi.org/10.3390/molecules27082494Shamsudin N.F. et al. Antibacterial Effects of Flavonoids and Their Structure-Activity Relationship Study: A Comparative Interpretation. Molecules. 2022; 27(4): 1149. https://doi.org/10.3390/molecules27041149Shamsudin N.F. et al. Antibacterial Effects of Flavonoids and Their Structure-Activity Relationship Study: A Comparative Interpretation. Molecules. 2022; 27(4): 1149. https://doi.org/10.3390/molecules27041149Kim D., Kuppusamy P., Jung J.S., Kim K.H., Choi K.C. Microbial Dynamics and In Vitro Degradation of Plant Secondary Metabolites in Hanwoo Steer Rumen Fluids. Animals. 2021; 11(8): 2350. https://doi.org/10.3390/ani11082350Kim D., Kuppusamy P., Jung J.S., Kim K.H., Choi K.C. Microbial Dynamics and In Vitro Degradation of Plant Secondary Metabolites in Hanwoo Steer Rumen Fluids. Animals. 2021; 11(8): 2350. https://doi.org/10.3390/ani11082350Phale P.S., Malhotra H., Shah B.A. Degradation strategies and associated regulatory mechanisms/features for aromatic compound metabolism in bacteria. Advances in Applied Microbiology. 2020; 112: 1–65. https://doi.org/10.1016/bs.aambs.2020.02.002Phale P.S., Malhotra H., Shah B.A. Degradation strategies and associated regulatory mechanisms/features for aromatic compound metabolism in bacteria. Advances in Applied Microbiology. 2020; 112: 1–65. https://doi.org/10.1016/bs.aambs.2020.02.002Vasconcelos N.G., Croda J., Simionatto S. Antibacterial mechanisms of cinnamon and its constituents: A review. Microbial Pathogenesis. 2018; 120: 198–203. https://doi.org/10.1016/j.micpath.2018.04.036Vasconcelos N.G., Croda J., Simionatto S. Antibacterial mechanisms of cinnamon and its constituents: A review. Microbial Pathogenesis. 2018; 120: 198–203. https://doi.org/10.1016/j.micpath.2018.04.036Lai T., Sun Y., Liu Y., Li R., Chen Y., Zhou T. Cinnamon Oil Inhibits Penicillium expansum Growth by Disturbing the Carbohydrate Metabolic Process. Journal of Fungi. 2021; 7(2): 123. https://doi.org/10.3390/jof7020123Lai T., Sun Y., Liu Y., Li R., Chen Y., Zhou T. Cinnamon Oil Inhibits Penicillium expansum Growth by Disturbing the Carbohydrate Metabolic Process. Journal of Fungi. 2021; 7(2): 123. https://doi.org/10.3390/jof7020123Slikkerveer A., de Wolff F.A. Toxicity of Bismuth and Its Compounds. Chang L.W. (ed.). Toxicology of Metals. Boca Raton: CRC Press. 1996; 1: 439–454. https://doi.org/10.1201/9781003418917-39Slikkerveer A., de Wolff F.A. Toxicity of Bismuth and Its Compounds. Chang L.W. (ed.). Toxicology of Metals. Boca Raton: CRC Press. 1996; 1: 439–454. https://doi.org/10.1201/9781003418917-39Zhao Z. et al. Pathway for biodegrading coumarin by a newly isolated Pseudomonas sp. USTB-Z. World Journal of Microbiology and Biotechnology. 2021; 37: 89. https://doi.org/10.1007/s11274-021-03055-wZhao Z. et al. Pathway for biodegrading coumarin by a newly isolated Pseudomonas sp. USTB-Z. World Journal of Microbiology and Biotechnology. 2021; 37: 89. https://doi.org/10.1007/s11274-021-03055-wАтландерова К.Н., Власенко Л.В., Дускаев Г.К. Оценка действия «Биовита» и коричного альдегида на степень переваримости корма и микробиом рубца крупного рогатого скота. Труды Кубанского государственного аграрного университета. 2024; 112: 201–207. https://elibrary.ru/enbrhlAtlanderova K.N., Vlasenko L.V., Duskaev G.K. Assessment of the “Biovit” and cinnamaldehyde effect on the degree of feed digestability and the cattle rumen microbiome. Proceedings of the Kuban State Agrarian University. 2024; 112: 201–207 (in Russian). https://elibrary.ru/enbrhlВласенко Л.В., Атландерова К.Н., Дускаев Г.К. Анализ микробиома рубца крупного рогатого скота и степени переваримости корма под действием умбеллиферона. Ветеринария и кормление. 2024; (5): 19–21. https://doi.org/10.30917/ATT-VK-1814-9588-2024-5-4Vlasenko L.V., Atlanderova K.N., Duskaev G.K. Analysis of the microbiome of the rumen of cattle and the degree of digestibility of feed under the influence of umbelliferone. Veterinaria i kormlenie. 2024; (5): 19–21 (in Russian). https://doi.org/10.30917/ATT-VK-1814-9588-2024-5-4

The authors declare that there are no conflicts of interest present.