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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vetpress</journal-id><journal-title-group><journal-title xml:lang="ru">Аграрная наука</journal-title><trans-title-group xml:lang="en"><trans-title>Agrarian science</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0869-8155</issn><issn pub-type="epub">2686-701X</issn><publisher><publisher-name>Редакция журнала "Аграрная наука"</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.32634/0869-8155-2025-394-05-63-68</article-id><article-id custom-type="elpub" pub-id-type="custom">vetpress-3621</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ЗООТЕХНИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>ZOOTECHNICS</subject></subj-group></article-categories><title-group><article-title>Особенности усвоения питательных веществ и химических элементов бычками при включении в рацион фермента протеазы</article-title><trans-title-group xml:lang="en"><trans-title>Features of the absorption of nutrients and chemical elements by gobies when the protease enzyme is included in the diet</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Шейда</surname><given-names>Е. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Sheida</surname><given-names>E. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Елена Владимировна Шейда, доктор биологических наук, ведущий научный сотрудник; доктор биологических наук, старший научный сотрудник</p><p>ул. 9 Января 29, Оренбург, 460000</p><p>пр-т Победы, 13, Оренбург, 460018</p></bio><bio xml:lang="en"><p>Elena Vladimirovna Sheida, Doctor of Biological Sciences, Leading Researcher; Doctor of Biological Sciences, Senior Researcher</p><p>29 9th January Str., Orenburg, 460000</p><p>13 Pobedy Ave., Orenburg, 460018</p></bio><email xlink:type="simple">elena-shejjda@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-9015-8367</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Дускаев</surname><given-names>Г. К.</given-names></name><name name-style="western" xml:lang="en"><surname>Duskaev</surname><given-names>G. K.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Галимжан Калиханович Дускаев, доктор биологических наук, профессор РАН, ведущий научный сотрудник</p><p>ул. 9 Января 29, Оренбург, 460000</p></bio><bio xml:lang="en"><p>Galimzhan Kalihanovich Duskaev, Doctor of Biological Sciences, professor of Russian Academy of Sciences, Leading Researcher</p><p>29 9th January Str., Orenburg, 460000</p></bio><email xlink:type="simple">gduskaev@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-0561-7002</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кван</surname><given-names>О. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Kvan</surname><given-names>O. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ольга Вилориевна Кван, кандидат биологических наук, старший научный сотрудник; кандидат биологических наук, старший научный сотрудник</p><p>ул. 9 Января 29, Оренбург, 460000</p><p>пр-т Победы, 13, Оренбург, 460018</p></bio><bio xml:lang="en"><p>Olga Vilorievna Kvan, Candidate of Biological Sciences, Senior Researcher; Candidate of Biological Sciences, Senior Researcher</p><p>29 9th January Str., Orenburg, 460000</p><p>13 Pobedy Ave., Orenburg, 460018</p></bio><email xlink:type="simple">kwan111@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-1622-1284</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Букарева</surname><given-names>Е. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Bukareva</surname><given-names>E. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Елена Анатольевна Букарева, кандидат биологических наук, старший научный сотрудник</p><p>ул. 9 Января 29, Оренбург, 460000</p></bio><bio xml:lang="en"><p>Elena Anatolyevna Bukareva, Candidate of Biological Sciences, Senior Researcher</p><p>29 9th January Str., Orenburg, 460000</p></bio><email xlink:type="simple">elenka_rs@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0009-5920-1185</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Сечнев</surname><given-names>Ю. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Sechnev</surname><given-names>Yu. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Юрий Андреевич Сечнев, аспирант</p><p>ул. 9 Января 29, Оренбург, 460000</p></bio><bio xml:lang="en"><p>Yuri Andreevich Sechnev, Postgraduate Student</p><p>29 9th January Str., Orenburg, 460000</p></bio><email xlink:type="simple">y.sechnev@outlook.com</email><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Федеральный научный центр биологических систем и агротехнологий Российской академии наук; Оренбургский государственный университет<country>Россия</country></aff><aff xml:lang="en">Federal Scientific Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences; Orenburg State University<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru">Федеральный научный центр биологических систем и агротехнологий Российской академии наук<country>Россия</country></aff><aff xml:lang="en">Federal Scientific Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2025</year></pub-date><pub-date pub-type="epub"><day>16</day><month>05</month><year>2025</year></pub-date><volume>0</volume><issue>5</issue><fpage>63</fpage><lpage>68</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Шейда Е.В., Дускаев Г.К., Кван О.В., Букарева Е.А., Сечнев Ю.А., 2025</copyright-statement><copyright-year>2025</copyright-year><copyright-holder xml:lang="ru">Шейда Е.В., Дускаев Г.К., Кван О.В., Букарева Е.А., Сечнев Ю.А.</copyright-holder><copyright-holder xml:lang="en">Sheida E.V., Duskaev G.K., Kvan O.V., Bukareva E.A., Sechnev Y.A.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.vetpress.ru/jour/article/view/3621">https://www.vetpress.ru/jour/article/view/3621</self-uri><abstract><sec><title>Актуальность</title><p>Актуальность. Питательные вещества в кормах влияют на содержание, рост и воспроизводство животных. Дефицит основных питательных веществ в кормах обычно компенсируется добавками. Среди таких добавок широкое применение находят протеазы. Протеазы способствуют более эффективному расщеплению белков корма на аминокислоты, которые затем легко усваиваются организмом, что особенно важно для молодых животных, нуждающихся в достаточном количестве аминокислот для поддержания интенсивного роста. Оптимизация усвоения аминокислот при помощи протеаз может привести к повышению эффективности использования корма, увеличению привесов и улучшению общего состояния здоровья бычков. Кроме того, это может снизить зависимость от дорогостоящих белковых добавок в рационе.</p><p>Цель исследования — изучение особенностей усвоения аминокислот бычками при включении в рацион протеазы.</p></sec><sec><title>Материалы и методы</title><p>Материалы и методы. Объекты исследования: рубцовая жидкость (РЖ), химус и кал бычков казахской белоголовой породы.</p></sec><sec><title>Результаты</title><p>Результаты. Анализ результатов показал, что внесение протеаз (грибной и бактериальной) способствует лучшей усвояемости аминокислот в организме бычков. Большую активность проявила протеаза бактериальная (II группа), что подтверждается данными увеличения усвояемости лизина (на 12,5%), фенилаланина (на 12,3%), тирозина (на 9,2%), серина (на 8,9%), треонина (на 8,8%), аланина (на 8,1%), изолейцина и глицина (на 7,6%), валина (на 5,9%), пролина (на 2,3%) и аргинина (на 2,9%) относительно контрольной группы.</p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Relevance</title><p>Relevance. Nutrients in feed affect animal maintenance, growth and reproduction. Deficiencies of essential nutrients in feed are usually compensated by additives. Among such additives, proteases are widely used. Proteases facilitate more efficient breakdown of feed proteins into amino acids that are then easily digested by the body, which is especially important for young animals that need sufficient amino acids to support intensive growth. Optimising amino acid digestion by proteases can lead to improved feed utilisation efficiency, increased weight gains and better overall health of steers. In addition, it may reduce dependence on expensive protein supplements in the diet.</p><p>The aim of the study was to study the peculiarities of amino acid absorption by bullheads when protease is included in the diet.</p></sec><sec><title>Materials and methods</title><p>Materials and methods. Objects of the study: rumen fluid (RF), chyme and faeces of steers of the Kazakh white-headed breed.</p></sec><sec><title>Results</title><p>Results. Analysis of the results showed that the introduction of proteases (fungal and bacterial) promotes better absorption of amino acids in the body of bulls. Bacterial protease (group II) showed the greatest activity, which is confirmed by the data of increase in assimilation of lysine (by 12,5%), phenylalanine (by 12,3%), tyrosine (by 9,2%), serine (by 8,9%), threonine (by 8,8%), alanine (by 8,1%), isoleucine and glycine (by 7,6%), valine (by 5,9%), proline (by 2,3%) and arginine (by 2,9%), relative to the control group.</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>крупный рогатый скот</kwd><kwd>экзоферменты</kwd><kwd>кормление</kwd><kwd>протеаза</kwd><kwd>аминокислоты</kwd><kwd>химус</kwd></kwd-group><kwd-group xml:lang="en"><kwd>cattle</kwd><kwd>exoenzymes</kwd><kwd>feeding</kwd><kwd>protease</kwd><kwd>amino acids</kwd><kwd>chyme</kwd></kwd-group><funding-group xml:lang="ru"><funding-statement>Исследование выполнено за счет средства гранта Российского научного фонда № 23-16-00061. https://rscf.ru/project/23-26-00061/</funding-statement></funding-group><funding-group xml:lang="en"><funding-statement>The research was carried out at the expense of the grant of the Russian Science Foundation No. 23-16-00061. https://rscf.ru/project/23-26-00061/</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Lukuyu B., Gachuiri C.K., Lukuyu M.N., Lusweti C., Mwendia S. 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