The article represents a hybridological analysis with the aim of studying the character of inheritance and identification of new allelic blocks of the components of gliadin-encoding loci in F2 hybrid grains obtained from interspecific crosses of durum wheat sample v. hordeiforme GR 8670 and a sample of a tetraploid wheat species T. dicoccum v. farum (Azerbaijan). The research is based on the hybrid grains F2 obtained from the crossing of local durum wheat GR 8670 v. hordeiforme (2n = 4x = 28) with a local sample of a cultivated spelled gingerbread (T. dicoccum v. farum, 2n = 4x = 28) with the Au Au BB genomic structure. The allelic blocks of the gliadin components have been identified in the electrophoregram of the gliadin storage proteins in 96 grains of the hybrid F2 generation by polymorphism and the frequency of occurrence of these alleles in the hybrid generation. The study of the hereditary nature of the alleles of gliadin-coding loci of storage proteins of F2 hybrids in the maternal form v. hordeiforme GR 8670 durum wheat made it possible to identify new allelic blocks Gld 6A9 and Gld 6A22. New allelic blocks of the Gld 6B19 and Gld 6B20 components were identified by the EF-analysis of the storage proteins of gliadin tetraploid wheat T. dicoccum v. farum (father form).