vetpressАграрная наукаAgrarian science0869-81552686-701XРедакция журнала "Аграрная наука"10.32634/0869-8155-2025-398-09-141-150vetpress-3837Research ArticleАГРОИНЖЕНЕРИЯ И ПИЩЕВЫЕ ТЕХНОЛОГИИAGROENGINEERING AND FOOD TECHNOLOGIESФерментативный гидролиз сельскохозяйственных растительных материалов с последующим культивированием кормовых дрожжей Candida tropicalis и Saccharomyces cerevisiaeEnzymatic hydrolysis of plant agricultural materials followed by cultivation of fodder yeasts Candida tropicalis and Saccharomyces cerevisiaehttps://orcid.org/0000-0002-6736-8788ПросвирниковД. Б.ProsvirnikovD. B.

Дмитрий Богданович Просвирников, доктор технических наук, профессор, главный научный сотрудник

ул. Карла Маркса, 68, Казань, 420015

Dmitry Bogdanovich Prosvirnikov, Doctor of Technical Sciences, Professor, Chief Researcher

68 Karl Marx Str., Kazan, 420015

prosvirnikov_dmi@mail.ruhttps://orcid.org/0000-0003-1842-5771ТунцевД. В.TuntsevD. V.

Денис Владимирович Тунцев, доктор технических наук, профессор, заведующий кафедрой, ведущий научный сотрудник

ул. Карла Маркса, 68, Казань, 420015

Denis Vladimirovich Tuntsev, Doctor of Technical Sciences, Professor, Head of Department, Leading Researcher

68 Karl Marx Str., Kazan, 420015

tuncev_d@mail.ruВалееваР. Т.ValeevaR. T.

Рауза Тимуровна Валеева, кандидат технических наук, доцент, старший научный сотрудник

ул. Карла Маркса, 68, Казань, 420015

Rauza Timurovna Valeeva, Candidate of Technical Sciences, Associate Professor, Senior Researcher

68 Karl Marx Str., Kazan, 420015

valeevaRT@corp.knrtu.ruИсмагиловаЛ. М.IsmagilovaL. M.

Лилия Масгутовна Исмагилова, кандидат технических наук, доцент, научный сотрудник

ул. Карла Маркса, 68, Казань, 420015

Liliya Masgutovna Ismagilova, Candidate of Technical Sciences, Associate Professor, Researcher

68 Karl Marx Str., Kazan, 420015

IsmagilovaLM@corp.knrtu.ruБродневаА. В.BrodnevaA. V.

Анна Владимировна Броднева, младший научный сотрудник

ул. Карла Маркса, 68, Казань, 420015

Anna Vladimirovna Brodneva, Junior Researcher

68 Karl Marx Str., Kazan, 420015

brodnevaAV@corp.knrtu.ruОдиловаР. М.OdilovaR. M.

Рахима Махмадкаримовна Одилова, лаборант

ул. Карла Маркса, 68, Казань, 420015

Rakhima Makhmadkarimovna Odilova, Laboratory Assistant

68 Karl Marx Str., Kazan, 420015

xkknitu@kstu.ruКазанский национальный исследовательский технологический университетРоссияKazan National Research Technological UniversityRussian Federation20252009202509141150Copyright © Просвирников Д.Б., Тунцев Д.В., Валеева Р.Т., Исмагилова Л.М., Броднева А.В., Одилова Р.М., 20252025Просвирников Д.Б., Тунцев Д.В., Валеева Р.Т., Исмагилова Л.М., Броднева А.В., Одилова Р.М.Prosvirnikov D.B., Tuntsev D.V., Valeeva R.T., Ismagilova L.M., Brodneva A.V., Odilova R.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vetpress.ru/jour/article/view/3837Актуальность

Актуальность. Растительные материалы — перспективный ресурс для производства кор­мового белка. Их переработка с помощью ферментативного гидролиза и культивирования дрожжей позволяет получать микробную биомассу с высокой пищевой ценностью. Это актуальное направление способствует решению проблемы дефицита кормового белка в животноводстве и устойчивому использованию ресурсов в условиях экологических и эко­номических вызовов.

Методы

Методы. Компонентный анализ сырья (лузга подсолнечника, пивная дробина, люпин, све­кловичный жом) проводили по стандартным методикам, сырье измельчали, сушили, под­вергали ферментативному гидролизу с применением ферментных препаратов ГК «Фер­мент» (Республика Беларусь) при оптимальных pH и температуре с контролем выхода са­харов, pH и конверсии. На полученных гидролизатах культивировали дрожжи S. cerevisiae и C. tropicalis и анализировали потенциал использования агроотходов.

Результаты

Результаты. При гидролизе пивной дробины получен максимальный выход сахаров (1,8%), у люпина — 1,7% (два пика выхода сахаров наблюдались в 2–4 и 7–9 ч.), у жома — 0,95% (пик в 4–5 ч.). При культивировании S. cerevisiae и C. tropicalis на гидролизатах пив­ной дробины и люпина дрожжи активнее росли на пивной дробине, достигая после сушки содержания протеина 68,18% и 72,63% а. с. в. и используя преимущественно при росте растворенные в гидролизате белковые фракции исходного сырья. Наиболее перспектив­ны гидролизаты пивной дробины и люпина, оптимальная продолжительность фермента­тивного гидролиза 6–10 ч.

Relevance

Relevance. Agro-food waste is a promising resource for the production of feed protein. Their processing using enzymatic hydrolysis and yeast cultivation allows obtaining microbial biomass with high nutritional value. This relevant area helps to solve the problem of feed protein deficiency in animal husbandry and sustainable use of resources in the context of environmental and economic challenges.

Methods

Methods. Component analysis of raw materials (sunflower husk, brewer’s grain, lupine, beet pulp) was carried out according to standard methods, the raw materials were crushed, dried, subjected to enzymatic hydrolysis using enzyme preparations of the Ferment Group of Companies (Republic of Belarus) at optimal pH and temperature, with control of sugar yield, pH and conversion. Yeasts S. cerevisiae and C. tropicalis were cultivated on the obtained hydrolysates and the potential for using agro-waste was analyzed.

Results

Results. The maximum sugar yield was obtained during the hydrolysis of brewer’s grains (1.8%), for lupine — 1.7% (two peaks of sugar yield were observed at 2–4 and 7–9 hours), for pulp – 0.95% (peak at 4–5 hours). When cultivating S. cerevisiae and C. tropicalis on hydrolysates of brewer’s grains and lupine, the yeast grew more actively on brewer’s grains, reaching a protein content of 68.18% and 72.63% a. d. w. after drying, and using mainly the protein fractions of the original raw material dissolved in the hydrolysate during growth. The most promising hydrolysates are brewer’s grains and lupine, the optimal duration of enzymatic hydrolysis is 6–10 hours.

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The authors declare that there are no conflicts of interest present.