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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vetpress</journal-id><journal-title-group><journal-title xml:lang="ru">Аграрная наука</journal-title><trans-title-group xml:lang="en"><trans-title>Agrarian science</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0869-8155</issn><issn pub-type="epub">2686-701X</issn><publisher><publisher-name>Редакция журнала "Аграрная наука"</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.32634/0869-8155-2025-399-10-197-202</article-id><article-id custom-type="elpub" pub-id-type="custom">vetpress-3876</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ЦИФРОВИЗАЦИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>DIGITALIZATION</subject></subj-group></article-categories><title-group><article-title>Цифровой двойник и предиктивное управление синтезом диизопропилового эфира в виброожиженном слое</article-title><trans-title-group xml:lang="en"><trans-title>Digital twin and predictive control of diisopropyl ether synthesis in a vibrofluidized bed</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Введенская</surname><given-names>А. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Vvedenskaya</surname><given-names>A. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Анна Васильевна Введенская - исследователь,</p><p>ул. Чапаевская, 89, Самара, 443099</p></bio><bio xml:lang="en"><p>Anna Vasilyevna Vvedenskaya - Researcher,</p><p>89 Chapaevskaya Str., Samara, 443099</p></bio><email xlink:type="simple">wasily10@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Глубокова</surname><given-names>М. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Glubokova</surname><given-names>M. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Мария Николаевна Глубокова - кандидат фармацевтических наук, доцент кафедрыобщей, бионеорганической и биоорганической химии,</p><p>ул. Чапаевская, 89, Самара, 443099</p></bio><bio xml:lang="en"><p>Maria Nikolaevna Glubokova - Candidate of Medical Sciences, Associate Professor,Department of General, Bioinorganic and Bioorganic Chemistry,</p><p>89 Chapaevskaya Str., Samara, 443099</p></bio><email xlink:type="simple">glubokova@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Самарский государственный медицинский университет Министерства здравоохранения Российской Федерации</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Samara State Medical University of the Ministry of Health Russian Federation</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2025</year></pub-date><pub-date pub-type="epub"><day>02</day><month>11</month><year>2025</year></pub-date><volume>0</volume><issue>10</issue><fpage>197</fpage><lpage>202</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Введенская А.В., Глубокова М.Н., 2025</copyright-statement><copyright-year>2025</copyright-year><copyright-holder xml:lang="ru">Введенская А.В., Глубокова М.Н.</copyright-holder><copyright-holder xml:lang="en">Vvedenskaya A.V., Glubokova M.N.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.vetpress.ru/jour/article/view/3876">https://www.vetpress.ru/jour/article/view/3876</self-uri><abstract><p>Представлена концепция использования ферментов семейства поли(АДФ-рибоза) полимераз (ПАРП) как инновационных биомаркеров для оценки репродуктивного потенциала петухов-производителей в промышленном птицеводстве. Разработана методология комплексного анализа активности ПАРП-1, ПАРП-2 и ПАРП-9 в сперматозоидах с целью повышения эффективности селекционного отбора. Экспериментально установлено, что концентрация интактных форм полимераз коррелирует с морфофункциональными характеристиками половых клеток (r = 0,73–0,81), тогда как уровень расщепленных форм обратно связан с жизнеспособностью сперматозоидов. Исследованы 248 петухов породы род-айланд в период 2023–2024 гг., показано превышение активности ПАРП в высококачественных образцах спермы на 60–62% по сравнению с низкокачественными. Разработана прогностическая модель с объяснительной способностью R2 = 0,71, позволяющая прогнозировать фертильный потенциал производителей с точностью 87%. Внедрение ПАРП-диагностики обеспечивает повышение точности селекционного отбора на 23–27%, снижение непродуктивных затрат на содержание низкокачественных самцов и ускорение создания конкурентоспособных отечественных линий птицы. Экономическая эффективность составляет 18,5 тыс. руб/га дополнительного дохода при окупаемости инвестиций 4,2 года.</p></abstract><trans-abstract xml:lang="en"><p>The concept of using enzymes from the poly(ADP-ribose) polymerase (PARP) family as innovative biomarkers for assessing the reproductive potential of breeder roosters in industrial poultry farming is presented. A methodology for the comprehensive analysis of PARP-1, PARP-2, and PARP-9 activity in spermatozoa has been developed to enhance the efficiency of breeding selection. It has been experimentally established that the concentration of intact forms of the polymerases correlates with the morphofunctional characteristics of germ cells (r = 0.73–0.81), while the level of cleaved forms is inversely related to sperm viability. A study of 248 Rhode Island Red roosters during 2023–2024 showed that PARP activity in highquality semen samples was 60-62% higher compared to low-quality samples. A predictive model with an explanatory power of R² = 0.71 was developed, allowing for the prediction of the fertile potential of breeders with an accuracy of 87%. The implementation of PARP-based diagnostics ensures a 23–27% increase in the accuracy of breeding selection, a reduction in unproductive costs associated with maintaining low-quality males, and an acceleration in the development of competitive domestic poultry lines. The economic efficiency amounts to 18.5 thousand RUB/ha of additional income with a return on investment of 4.2 years.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>цифровой двойник</kwd><kwd>предиктивное управление</kwd><kwd>виброожиженный слой</kwd><kwd>диизопропиловый эфир</kwd><kwd>каталитический синтез</kwd></kwd-group><kwd-group xml:lang="en"><kwd>digital twin</kwd><kwd>predictive control</kwd><kwd>vibrofluidized bed</kwd><kwd>diisopropyl ether</kwd><kwd>catalytic synthesis</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Saha P., Bruning H., Wagner T.V., Rĳnaarts H.H.M. Removal of organic compounds from cooling tower blowdown by electrochemical oxidation: Role of electrodes and operational parameters. 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