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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vetpress</journal-id><journal-title-group><journal-title xml:lang="ru">Аграрная наука</journal-title><trans-title-group xml:lang="en"><trans-title>Agrarian science</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0869-8155</issn><issn pub-type="epub">2686-701X</issn><publisher><publisher-name>Редакция журнала "Аграрная наука"</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.32634/0869-8155-2025-400-11-76-82</article-id><article-id custom-type="elpub" pub-id-type="custom">vetpress-3896</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ЗООТЕХНИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>ZOOTECHNICS</subject></subj-group></article-categories><title-group><article-title>Влияние различных временных режимов проведения сеансов OPU на развитие овариальных фолликулов у тёлок сычёвской породы</article-title><trans-title-group xml:lang="en"><trans-title>The influence of different time modes of OPU sessions on the development of ovarian follicles in heifers of the Sychev breed</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0002-2861-1834</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Белокурова</surname><given-names>С. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Belokurova</surname><given-names>S. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p> София Сергеевна Белокурова, аспирант</p><p> пос. Дубровицы, 60, г. о. Подольск, Московская обл., 14213 </p></bio><bio xml:lang="en"><p> Sofia Sergeevna Belokurova, Graduate Student</p><p> 60 Dubrovitsy settlement, Podolsk, Moscow region, 142132 </p></bio><email xlink:type="simple">sofialabs@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-6511-5341</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Чинаров</surname><given-names>Р. Ю.</given-names></name><name name-style="western" xml:lang="en"><surname>Chinarov</surname><given-names>R. Yu.</given-names></name></name-alternatives><bio xml:lang="ru"><p> Роман Юрьевич Чинаров, кандидат ветеринарных наук, старший научный сотрудник</p><p> пос. Дубровицы, 60, г. о. Подольск, Московская обл., 14213 </p></bio><bio xml:lang="en"><p> Roman Yurievich Chinarov, Candidate of Veterinary Sciences, Senior Researcher </p><p> 60 Dubrovitsy settlement, Podolsk, Moscow region, 142132 </p></bio><email xlink:type="simple">roman_chinarov@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-4744-7873</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Луканина</surname><given-names>В. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Lukanina</surname><given-names>V. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p> Виктория Александровна Луканина, кандидат биологических наук, старший научный сотрудник</p><p> пос. Дубровицы, 60, г. о. Подольск, Московская обл., 14213 </p></bio><bio xml:lang="en"><p> Viktoria Alexandrovna Lukanina, Candidate of Biological Sciences, Senior Researcher </p><p> 60 Dubrovitsy settlement, Podolsk, Moscow region, 142132 </p></bio><email xlink:type="simple">kristybatle@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-0198-9757</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Сингина</surname><given-names>Г. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Singina</surname><given-names>G. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p> Галина Николаевна Сингина, кандидат биологических наук, ведущий научный сотрудник</p><p> пос. Дубровицы, 60, г. о. Подольск, Московская обл., 14213 </p></bio><bio xml:lang="en"><p> Galina Nikolaevna Singina, Candidate of Biological Sciences, Leading Researcher</p><p> 60 Dubrovitsy settlement, Podolsk, Moscow region, 142132 </p></bio><email xlink:type="simple">g_singina@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Федеральный исследовательский центр животноводства — ВИЖ им. академика Л.К. Эрнста</institution><country>Россия</country></aff><aff xml:lang="en"><institution>L.K. Ernst Federal Research Center for Animal Husbandry</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2025</year></pub-date><pub-date pub-type="epub"><day>19</day><month>11</month><year>2025</year></pub-date><volume>0</volume><issue>11</issue><fpage>76</fpage><lpage>82</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Белокурова С.С., Чинаров Р.Ю., Луканина В.А., Сингина Г.Н., 2025</copyright-statement><copyright-year>2025</copyright-year><copyright-holder xml:lang="ru">Белокурова С.С., Чинаров Р.Ю., Луканина В.А., Сингина Г.Н.</copyright-holder><copyright-holder xml:lang="en">Belokurova S.S., Chinarov R.Y., Lukanina V.A., Singina G.N.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.vetpress.ru/jour/article/view/3896">https://www.vetpress.ru/jour/article/view/3896</self-uri><abstract><p>С учетом влияния большого количества факторов на результативность трансвагинальной УЗИ-ассистированной аспирации овариальных фолликулов (OPU), среди которых породные и индивидуальные особенности животных, необходим подбор оптимальных режимов проведения OPU для разных пород крупного рогатого скота. Стоит отметить, что особенно актуальным является изучение параметров, влияющих на проведение технологии OPU/IVP, в рамках создания криобанков эмбрионов отечественных пород крупного рогатого скота, так как они имеют ограниченную численность. Данное исследование было проведено в ФГБНУ ФИЦ ВИЖ им. Л.К. Эрнста на тёлках сычёвской породы, признанной генофондной.Цель работы — изучение влияния кратности проведения сеансов OPU (1 или 2 раза в неделю) на паттерн развития овариальных фолликулов во взаимосвязи с количеством и качеством извлекаемых ооцитов.В ходе исследования было установлено, что при уменьшении интервала между сеансами OPU c 7 до 3–4 суток происходило увеличение (на 73,8%) числа аспирированных фолликулов среднего размера с одновременным уменьшением числа и доли дегенерированных ооцитов. Так, в группе, подвергающейся OPU дважды в неделю, доля пригодных ооцитов составляла 62,8% против 53,3% в группе, в которой сеансы проводили один раз в неделю. При этом доля аспирированных фолликулов от общего числа УЗИ-видимых фолликулов между группами не различалась (78,6% против 78,4%). С учетом полученных результатов оптимальным режимом проведения сеансов OPU на тёлках сычёвской породы было принято считать проведение процедуры два раза в неделю.</p></abstract><trans-abstract xml:lang="en"><p>Since the effectiveness of OPU is influenced by a large number of factors, including the breed and individual characteristics of animals, it is necessary to select the optimal OPU modes for different breeds of cattle. It is worth noting that it is particularly relevant to study the factors affecting the effectiveness of OPU/IVP in the framework of creating cryobanks of embryos of domestic cattle breeds, since they have a limited number. This study was conducted at the L.K. Ernst Federal Research Center for Animal Husbandry on heifers of the Sychev breed recognized by the gene pool.The aim of the work was to study the effect of the frequency of OPU sessions (1 or 2 times a week) on the pattern of ovarian follicle development in relation to the quantity and quality of extracted oocytes.During the study, it was found that with a decrease in the interval between OPU sessions from 7 to 3–4 days, there was an increase in the number of medium-sized aspirated follicles (by 73.8%) with a simultaneous decrease in the number and proportion of degenerated oocytes. Thus, in the group undergoing OPU twice a week, the proportion of usable oocytes was 62.8% versus 53.3% in the group in which the sessions were performed once a week. At the same time, the proportion of aspirated follicles from the total number of ultrasound-visible follicles did not differ between the groups (78.6% versus 78.4%). Taking into account the results obtained, the optimal mode of conducting OPU sessions on Sychev heifers was considered to be twice a week.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>вспомогательные репродуктивные технологии</kwd><kwd>OPU</kwd><kwd>ычевская порода</kwd><kwd>УЗИ</kwd><kwd>ооцит-кумулюсные комплексы</kwd><kwd>размер фолликулов</kwd><kwd>тёлки-доноры</kwd></kwd-group><kwd-group xml:lang="en"><kwd>assisted reproductive technologies</kwd><kwd>OPU</kwd><kwd>Sychev breed</kwd><kwd>ultrasound</kwd><kwd>cumulusoocyte complexes</kwd><kwd>follicle size</kwd><kwd>donor-heifers</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Исследование выполнено при поддержке Минобрнауки России (соглашение № 075-02-2025-1585).</funding-statement><funding-statement xml:lang="en">This research was funded by the Ministry of Science and Higher Education of Russia (аgreement No. 075-02-2025-1585).</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Pieterse M.C, Kappen K.A., Kruip T.A.M., Taverne M.A.M. 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