vetpressАграрная наукаAgrarian science0869-81552686-701XРедакция журнала "Аграрная наука"10.32634/0869-8155-2025-401-12-93-98vetpress-3940Research ArticleЗООТЕХНИЯZOOTECHNICSВлияние различных концентраций инсулиноподобного фактора роста 1 на in vitro созревание ооцитов и последующее развитие партеногенетических эмбрионов у крупного рогатого скотаThe effect of different concentrations of insulin-like growth factor-1 on in vitro maturation of oocytes and subsequent development of parthenogenetic embryos in cattlehttps://orcid.org/0000-0003-0198-9757СингинаГ. Н.SinginaG. N.

Галина Николаевна Сингина, кандидат биологических наук, заведующая лабораторией эмбриональных технологий 

Дубровицы, 60, г. о. Подольск, Московская обл., 142132 

Galina Nikolaevna Singina, Candidate of Biological Sciences, Head of the Laboratory of Embryonic Technologies 

60 Dubrovitsy, Podolsk Municipal District, Moscow Region, 142132 

g_singina@mail.ruhttps://orcid.org/0000-0002-9642-2384ШедоваЕ. Н.ShedovaE. N.

Екатерина Николаевна Шедова, научный сотрудник 

Дубровицы, 60, г. о. Подольск, Московская обл., 142132 

Ekaterina Nikolaevna Shedova, Research Associate 

60 Dubrovitsy, Podolsk Municipal District, Moscow Region, 142132 

shedvek@yandex.ruhttps://orcid.org/0000-0002-1284-1486ЛопуховА. В.LopukhovA. V.

Александр Викторович Лопухов, научный сотрудник 

Дубровицы, 60, г. о. Подольск, Московская обл., 142132 

Alexandеr Viktorovich Lopukhov, Research Associate 

60 Dubrovitsy, Podolsk Municipal District, Moscow Region, 142132 

vubi_myaso@mail.ruФедеральный исследовательский центр животноводства — ВИЖ им. академика Л.К. ЭрнстаРоссияL.K. Ernst Federal Research Center for Animal HusbandryRussian Federation2025131220250129398Copyright © Сингина Г.Н., Шедова Е.Н., Лопухов А.В., 20252025Сингина Г.Н., Шедова Е.Н., Лопухов А.В.Singina G.N., Shedova E.N., Lopukhov A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vetpress.ru/jour/article/view/3940Актуальность

Актуальность. Инсулиноподобный фактор роста 1 (insulin-like growth factor-1, IGF-1) вовлечен in vivo в регуляцию мейоза, в связи с чем активно изучается как потенциальный регулятор качества ооцитов с целью повышения эффективности биотехнологий, направленных на получение эмбрионов in vitro. В настоящей работе исследовали влияние IGF-1 в культуре ооцитов коров с точки зрения их созревания in vitro и эмбрионального развития после искусственной активации, а также качества полученных эмбрионов.

Методы

Методы. Выделенные из овариальных фолликулов ооциты культивировали в отсутствие (контроль) и присутствии различных концентраций (5, 10, 20, 40 и 80 нг/мл) IGF-1. Затем созревшие ооциты искусственно активировали и культивировали в течение 7 дней для эмбрионального развития.

Результаты

Результаты. IGF-1 не повлиял на созревание ооцитов, а также на их дробление после партеногенетической активации и число ядер в 7-дневных эмбрионах. В то же время обнаружен зависящий от концентрации эффект IGF-1 на развитие ооцитов до стадии бластоцисты. В контроле выход бластоцист составлял 17,3±1,6%. Присутствие в среде созревания IGF-1 в концентрациях 5, 10 и 20 нг/мл повышало этот показатель, соответственно, до 29,9±4,5, 30,7±3,6 и 29,6±2,3% (p < 0,05). Следовательно, в этих концентрациях IGF-1 может быть рекомендован для in vitro созревания ооцитов коров. 

Relevance

Relevance. Insulin-like growth factor-1 (IGF-1) is involved in the regulation of meiosis in vivo and is therefore actively investigated as a potential regulator of oocyte quality aimed at improving the efficiency of in vitro embryo production biotechnologies. In the present study, we examined the effect of IGF-1 during bovine oocyte culture in terms of their in vitro maturation, embryonic development after artificial activation, and the quality of the resulting embryos.

Materials and methods

Materials and methods. Oocytes isolated from ovarian follicles were cultured either in the absence (control) or presence of various concentrations of IGF-1 (5, 10, 20, 40, and 80 ng/ml). Matured oocytes were then artificially activated and cultured for 7 days to support embryonic development.

Results

Results. IGF-1 had no effect on oocyte maturation, cleavage, or the number of nuclei in blastocyst. At the same time, a concentration-dependent effect of IGF-1 on the development of oocytes to the blastocyst stage was observed. In the control group, the blastocyst yield was 17.3±1.6%. The presence of IGF-1 in the maturation medium at concentrations of 5, 10, and 20 ng/ml increased this rate to 29.9±4.5, 30.7±3.6, and 29.6±2.3%, respectively (p < 0.05). Thus, at these concentrations IGF-1 can be recommended for in vitro maturation of bovine oocytes. 

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The authors declare that there are no conflicts of interest present.