Development of a polish lacticcontaining probiotic supplement Gallobact-F
https://doi.org/10.32634/0869-8155-2020-340-7-24-28
Abstract
Relevance and methods. The probiotic properties of three strains of bacteria – typical representatives of lactobacilli – have been studied.
Results. As a result, it was revealed that all strains of lactobacilli are resistant to acid and bile, «resistant» to the action of therapeutic doses of antibiotics, and also exhibit high antagonistic properties in relation to E. coli ATCC 25922, P. aeruginosa ATCC 27853 and S. aureus ATCC 25923. At the same time, Lactobacillus intermedius was the leader in active acid formation, Lactobacillus salivarius was distinguished by an average level of adhesive activity and high anti-adhesive properties. In addition, the selected strains of lactobacilli are safe to use. Convinced of the effectiveness of the selected strains of lactobacilli, we have developed a technological process for the production of probiotic supplements Gallobact-F, which includes several stages.
About the Authors
V. V. ShkredovRussian Federation
Vladimir V. Shkredov - post-graduate student of the Department of Biotechnology, Biochemistry and Biophysics
A. G. Koschaev
Russian Federation
Andrey G. Koschaev - Doc. Sci. (Biology), Professor, Vice-Rector for Research
K. N. Murtazaev
Russian Federation
Kurban N. Murtazaev - post-graduate student of the Department of Biotechnology, Biochemistry and Biophysics
Y. A. Yuldashbaev
Russian Federation
Yusup A. Yuldashbaev - Doc. Sci. (Agriculture), Professor, Dean of the faculty of animal science and biology
N. A. Sergeenkova
Russian Federation
Nadezhda A. Sergeenkova - post-graduate student of the Department of animal physiology, ethology and biochemistry
References
1. Alekseev A.L., Kryshtop E.A., Vasilenko A.Yu. Meat qualities of pigs when using probiotics in diets. News of the Nizhnevolzhsky agricultural university complex: science and higher professional education. 2011;3(23):151–156. [in Russian]
2. Baltrashevich A.K., Komarovskaya T.P. Method for determining the sensitivity of anaerobic microorganisms to antibiotics using standard discs. Antibiotics. 1982;(8):32–36. [in Russian]
3. Voitenko O.S., Baranikov V.A., Borilo O.R. Biological preparations in pig breeding. Veterinary pathology. 2013;(3):14–17. [in Russian]
4. Darmov I.V., Marakulin I.V., Pogorelsky I.P., Pozolotina N.V. Isolation and comparative characteristics of strains of lactobacilli – representatives of normal intestinal microflora of pigs. Actual problems of veterinary biology. 2015;2(26):3–10. [in Russian]
5. Gamko L.N., Sidorov I.I., Talyzina T.L. Probiotics in feeding young pigs. Feeding of agricultural animals and fodder production. 2012;(11):33–41. [in Russian]
6. Ganieva, S.R. Growth and development of pigs using the probiotic “Sporovit” in industrial technology: diss. ... сand. sciences: 06.02.10 / Ganieva Sariya Raisovna. Ufa, 2015. 135 p. [in Russian]
7. Dubskaya, E.I. Increasing the efficiency of poultry production based on the use of probiotics. Bulletin of the Orenburg State Agrarian University. 2008;1(17-10):135–136. [in Russian]
8. Kitaevskaya, S.V. Modern trends in selection and identification of probiotic strains of lactic acid bacteria [Electronic resource]. Bulletin of Kazan Technological University. 2012. Р.184–188. URL: https://cyberleninka.ru/article/n/sovremennye-tendentsiiotbora-i-identifikatsii-probioticheskih-shtammov-molochnokislyh-bakteriy. [in Russian]
9. Labinskaya, A.S. Microbiology with the technique of microbiological research. M.: Medicine, 1978. 394 p. [in Russian]
10. Levakhin, V.I. Probiotics in animal husbandry. Bulletin of meat cattle breeding. – 2013;1(79):7–10. [in Russian]
11. Malik, N.I. Probiotics and their influence on the growth and safety of broilers. Bio. – 2002;(3):8. [in Russian]
12. Makhalov A.G., Shulgin S.V. The use of probiotics in the diets of goslings broilers. Feeding of agricultural animals and fodder production. 2012;(12):51–58. [in Russian]
13. MU 2.3.2.2789-10. Guidelines for the sanitary and epidemiological assessment of safety and functional potential of probiotic microorganisms used for food production: Guidelines. Moscow: Federal Center for Hygiene and Epidemiology of Rospotrebnadzor, 2011. 104 p. [in Russian]
14. Nekrasov R.V., Kirilov M.P., Ushakova N.A. The use of new generation probiotics in feeding pigs. Problems of biology of productive animals. 2010;(3):64–79. [in Russian]
15. Pyshmantseva N.A., Chikov A.E., Osepchuk D.V., Kovekhova N.P. On the effectiveness of the earliest possible use of probiotics in chickens of egg breeds. Problems of biology of productive animals. 2011;(1):93–99. [in Russian]
16. Kuzminova E.V., Semenenko M.P., Starikova E.A., Tyapkina E.V. The use of biologically active substances for the normalization of metabolic processes in animals. Bulletin of the Altai State Agrarian University. 2013;11(109):80–83. [in Russian]
17. Chabaev M., Anisova N., Nekrasov R., Grishchenko V. Probiotic complex for calves. Compound feed. 2013;(7):47–48. [in Russian]
18. Smolyanikov, Yu.I., Bely D.S. Probiotic preparation in the rations of cows. Feeding of agricultural animals and fodder production. 2011;(3):43–45. [in Russian]
19. Sokolenko, G.G., Lazarev B.P., Minchenko S.V. Probiotics in rational feeding of animals. Technologies of food and processing industry of the agro-industrial complex – healthy food products. 2015;(1):72–78. [in Russian]
20. Talyzina, T.L., Gamko L.N., Anokhina V.D. Physiological and biochemical substantiation of the effectiveness of using a probiotic supplement for fattening pigs. Bulletin of the Bryansk State Agricultural Academy. 2013;(3):31–33. [in Russian]
21. Tepper, E.Z., Shilnikova V.K., Pereverzeva G.I.; ed. Shilnikova V.K. Laboratory workshop on microbiology. 5th ed., Rev. and add. M.: Drofa, 2004. 256 p. [in Russian]
22. Shenderov, B.A. Probiotics and functional nutrition. M.: Grant, 2001. 288 p. [in Russian]
23. Yarullina, D.R., Fakhrullin R.F. Bacteria of the genus Lactobacillus: general characteristics and methods of working with them: study guide. allowance. Kazan: Kazan University, 2014. 51 p. [in Russian]
24. Yu Y., Amorim C., Marques C., Calhau M. Effects of whey peptide extract on the growth of probiotics and gut microbiota. Journal of Functional Foods. 2016, March. P.507–516.
25. Holzapfel, W.H., Shulinger U. Introduction to preand probiotics. Food Research International. 2002;(35):125–129.
26. Liu, H.F., Ji D.Y., Zhang S.X. Effects of Lactobacillus brevis preparation on growth performance, fecal microflora and serum profile in weaned pigs. Livestock Science. 2015. P.251–254.
Review
For citations:
Shkredov V.V., Koschaev A.G., Murtazaev K.N., Yuldashbaev Y.A., Sergeenkova N.A. Development of a polish lacticcontaining probiotic supplement Gallobact-F. Agrarian science. 2020;(7-8):24-28. (In Russ.) https://doi.org/10.32634/0869-8155-2020-340-7-24-28