Formation of a system of genetic certification of linseed

Relevance. The study of the genetic diversity of linseed and the clear identification of cultivars of this crop is an important part of breeding work. DNA marking, including the use of microsatellite SSR markers, is considered to be the most effective method for analyzing genetic polymorphism and improving the efficiency of selection. The purpose of this work was to study the polymorphism of a number of modern cultivars of linseed and develop their genetic passport.

Materials and methods. The object of research was 22 varieties of oilseed flax included in the state register, of various breeding origin. The genetic analysis was performed by PCR using a line of 11 SSR markers developed and synthesized by “Syntol” LLC, with subsequent detection of products on a genetic analyzer.

Results. In the study sample, 46 alleles were identified. Each sample contained a unique set of alleles. The use of alphanumeric code for SSR markers allowed the formation of genetic passports for each studied cultivar. This will allow accurate genotyping of morphologically difficult to distinguish samples, which shows the possibility of certification of all flax cultivars included in the State register of breeding achievements of the Russian Federation. Cluster analysis with the construction of a dendrogram of genetic similarity revealed differences in the studied samples. Cultivars of the studied samples were divided into three separate groups, two of which are characterized by origin and relationships between them, the third group of cultivars are the results of selection work using samples of different geographical origin.

1. Food and Agriculture Organization of the United Nations. [Electronic resource] - Electron. text data. - Access mode: http://www.fao.org/faostat/en/#data/QC, Free admission. - (date of access: 13.04.2020)

2. Andronik E.L., Maslinskaya M.E., Ivanova E.V The role of the oil flax gene pool in solving urgent problems of breeding, plant growing and improving the quality of life. Agricultural magazine. 2014. P.4. (In Russ.)

3. Cullis C.A. Genetics and genomics of linum. Plant genetics and genomics: crops and models, 2019;(23).

4. Egorov S.V, Porkhuntsova O.A. Variation of oil flax genotypes according to the criteria of internal polymorphism. Bulletin of the Belarusian State Agricultural Academy. 2019;(3):108-113. (In Russ.)

5. Porokhovinova E.A., Shelenga TV., Matveeva TV. Polymorphism of genes controlling the low content of linolenic acid in lines of the VIR flax genetic collection. Environmental genetics. 2019;17(2):5-19. (In Russ.)

6. Uschapovsky I.V., Lemesh V.A., Bogdanova M.V., Guzenko E.V. Features of breeding and prospects for the application of molecular genetic methods in genetic selection studies of flax (Linum usitatissimum L.). Agricultural biology. 2016;51(5):602-616. (In Russ.)

7. Wu J. et al. Development of novel SSR markers for flax (Linum usitatissimum L.) using reduced-representation genome sequencing. Frontiers in plant science. 2017;(7):2018.

8. Bazanov T.A., Ushchapovsky I.V., Lemesh V.A., Bogdanova M.V., Lagunovskaya E.V. Genetic polymorphism of modern fiber flax (Linum usitatissimum L.) varieties of Russian selection using SSR markers. Works on applied botany, genetics and selection. 2019;180(4):81-87. (In Russ.)DOI: 10.30901/2227-8834-20194-81-87

9. Nei M. Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences. USA. 1973. Р3321-3323.

10. Saitou N., Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 1987;4(4):406-425.

11. Roslenkonoplya. Characteristics of varieties of oil flax. [Electronic resource] - Electron. text data. - Access mode: https://www.rosflaxhemp.ru/fakti-i-cifri/spravochnie-materiali.html/id/10, Admission isfree. - (date of access: 07/14/2020) (In Russ.)

12. Agnews. Varieties and hybrids. [Electronic resource] -Electron. text data. - Access mode: https://agro-bursa.ru/gazeta/sorta-gibridy/archive/, Admission is free. - (date of access: 07/14/2020) (In Russ.)