Modern approaches to the production of inactivated vaccines against chicken cholera

Introduction. Chicken cholera is one of the most dangerous avian infectious diseases, causing significant economic damage to the industrial poultry production. Chicken cholera usually occurs in septic form, and causes high morbidity and mortality (60–80%), but recently it has become chronic, subclinical and associated. Inactivated emulsion vaccines are used worldwide to prevent chicken cholera and provide high and long-term immunity. However, there is a problem with residual reactogenicity of inactivated vaccines, particularly of the bacterial variants. This problem can be solved by using safer, next-generation adjuvants. The aim of the article is to study the physical and biological properties and determine the optimal inoculation volume and method of administration of inactivated vaccines against chicken cholera , based on different adjuvants.
Materials and methods. Formaldehyde inactivated culture of P. multocida st. 115and a number of adjuvants (“Montanide GEL-02” and oil adjuvants “Montanide ISA 70 VG” and “Montanide ISA 78 VG”) were used for vaccine production. The vaccine samples were tested for sterility, stability and viscosity by conventional methods. Determination of reactogenicity and antigenic activity of the vaccines was carried out on young 30-days old chickensof egg-laying type.
Results. The vaccine sample based on the adjuvant “Montanide ISA 70 VG” containing 1.5 billion P. Multocida microbial cells in a single immunizing dose of 0.3 cm3 was found to be the best among the tested preparations. When assessing the reactogenicity, it was obvious that all samples, regardless of the type of adjuvant, showed more pronounced residual reactogenic properties when injected intramuscularly into the chest muscle than when injected subcutaneously into the middle third of the neck.

1. Miftakhova A.V., Drozdova L.I., Nikitin A.P. Bird pasteurellosis. In digest: Diseases of Birds. Digest of articles. 2020: 148-150. (In Russian)

2. Korochkin R. Pasteurellosis, Pasteurella and related animal diseases. Veterinary business (Minsk). 2022; 1:17-23. (In Russian)

3. Kozhevnikov E.M. Bacteriocarrier, its importance in the ecology of Pasterella multocida and in the control of avian pasteurellosis. AutoAbstract doc. dis. Voronezh, 1975. (In Russian)

4. Rozhdestvenskaya T.N., Pankratov S.V., Ruzina A.V., Novikova O.B. Respiratory syndrome — an open gate for infection. Poultry and poultry products. 2020; 6: 40-42 (DOI: 10.30975/2073-4999-2020-22-6-40-42). (In Russian)

5. Gromov I. N., Zhurov D. O., Barshay E. A. Respiratory avian diseases: pathomorphology and diagnostics. Vitebsk, 2017. (In Russian)

6. Gromov I., Subbotina I., Kotsyuba E. Pathomorphology of acute septic bacterioses and viroses of birds occurring with a predominant lesion of the respiratory organs. Veterinary business (Minsk). 2022; 2:6-14. (In Russian)

7. Pankratov S.V., Sukhinin A.A., Rozhdestvenskaya T.N., Ruzina A.V. Respiratory Syndrome of Birds. Etiology, diagnosis, control and prevention measures. Poultry and poultry products. 2021; 4: 34-36 (DOI: 10.30975/2073-4999-2021-23-4-34-36). (In Russian)

8. Khaisanova V.S., Vasiliev D.A. Study of antibiotic resistance of P. Multocida bacteria. Modern Science: actual problems of theory and practice. Series: Natural and technical sciences. 2021; 5: 46-49. (In Russian)

9. Wentzel J.M., van Vuuren M., Biggs L.J. comparing the minimum inhibitory and mutant prevention concentrations of selected antibiotics against animal isolates of Pasteurella multocida and Salmonella typhimurium. Onderstepoort Journal of Veterinary Research. 2022; Т. 89. № 1.

10. Ahr A.D., Salib F.A.-A., Amin M.M., Soliman Y.A. Multi-drug resistant pasteurella multocida and mannheimia haemolytica strains isolated from different hosts affected by pneumonic pasteurellosis in Egypt. Advances in Animal and Veterinary Sciences. 2021; Т. 9. № 3: 356-364.

11. Saha O., Islam M.R., Rahman M.S., Hoque M.N., Hossain M.A., Sultana M. First report from bangladesh on genetic diversity of multidrug-resistant Pasteurella multocida type b:2 in fowl cholera. Veterinary World. 2021; Т. 14. № 9: 2527-2542.

12. Naqvi S.A.R., Kanwal L., Saeed M., Atta-ul-Haq, Shah S.M.A., Nisar J., Akram M., Nisar Z. Antimicrobial and antihypercholesterolemic activities of pulicaria gnaphalodes Dose-Response. 2020; Т. 18. № 1.

13. Biryuchenkova M.V., Timina A.M., Shcherbakov A.V. Gene diagnostics of diseases associated with Pasteurella multocida. In col.: Achievements of young scientists in veterinary practice. Vladimir, 2016: 192-198. (In Russian)

14. Van Dijk J.G.B., Iverson S.A., Gilchrist H.G., Buttler E.I., Forbes M.R., Hennin H.L., Harms N.J., Soos C., Love O.P., Lesceu S., Foster J.T. Herd immunity drives the epidemic fadeout of avian cholera in arctic-nesting seabirds. Scientific Reports. 2021; Т. 11. № 1: 1046.

15. Hoelzer, K. Bielke L., Blake D.P. Vaccines as alternatives to antibiotics for food producing an-imals. Part 2: new approaches and potential solutions. Veterinary Research. 2018; Vol. 49, № 1: 70.

16. Smallman T.R., Williams G.C., Harper M., Boyce J.D. Genome-wide investigation of Pasteurella multocida identifies the stringent response as a negative regulator of hyaluronic acid capsule production. Microbiology Spectrum. 2022; Т. 10. № 2.

17. Malik E.V., Malik N.I., Guleichik I.A., Chupakhina N.A., Malenkova L.A., Samokhvalova N.S. Analysis of problems with identification of Pasteurella multocida cultures by capsular groups and production of toxins , In the book: Infectious diseases in the modern world: evolution, current and future threats. Proceedings of the XIII Annual All-Russian Congress on Infectious Diseases named after academician V.I. Pokrovsky; IV All-Russian Scientific and Practical Conference; VI AllRussian Symposium. Moscow, 2021: 105. (In Russian)

18. Malik E.V., Malenkova L.A., Malik N.I., Rusanov I.A., Prasolova O.V. Phenotyping of strains and isolates of P. multocida by capsular groups in hyaluronidase and acriflavin tests. In the book: Topical issues of biology, biotechnology, veterinary medicine, animal science, commodity science and processing of raw materials of animal and vegetable origin. Moscow, 2021: 49-50. (In Russian)

19. Semina A.N. Study of the Pasteurella multocida genome for specific identification in the poultry industry. Efficient animal husbandry. 2020; 4 (161): 142-143. (In Russian)

20. Borisenkova A.N. The role of somatic and capsular antigens of P. multocida in the immunological specificity of vaccines. Veterinary medicine. 1978; 5: 40-42. (In Russian)

21. B. W. Calneck, H. J. Barnes, C. W. Bearda, L. R. McDougald, and I. M. Seifa, Diseases of poultry and farm birds. М., 2011. (In Russian)

22. Yang X., Wu Q., Zhang J. Prevalence, bacterial load, and antimicrobial resistance of Sal-monella serovars isolated from retail meat and meat products in China. Frontiers in Microbiology. 2019; 10: 2121.

23. Шемельков Е.В., Алипер Т.И., Куликова Т.С., Кунаков К.Ю., Булгаков А.Д., Верховский О.А. Подбор адъюванта для субъединичной маркированной вакцины против классической чумы свиней Ветеринария. 2022; 4: 32-40. (In Russian)

24. Rozhdestvenskaya T. N., Pankratov S. V., Sapegina E. V., Tomina E. V. Testing of new SEPPIC adjuvants for the production of vaccines against avian hemophiliasis. Veterinary medicine, zootechnics and biotechnology. 2020; 11: 23-27 (DOI: 10.26155/vet.zoo.bio.202011004). (In Russian)

25. Nikitin G., Pankratov S., Sukhinin A., Prikhod’ko E., Rozhdestvenskaya T., Ruzina A.Adjuvants for inactivated vaccine against avibacterium paragallinarum. FASEB Journal. 2022; Т. 36. — № S1. (DOI: 10.1096/fasebj.2022.36.S1.R3128)

26. Lakin G.F. Biometrics // M.: «High School». — 1990. — 352 с. (In Russian)