The ability of Bacillus mojavensis PS17 to grow and synthesize exogenous enzymes at low temperatures
https://doi.org/10.32634/0869-8155-2022-362-9-143-146
Abstract
Relevance. Microorganisms growing at low temperatures play a key role in the biochemical cycles in cold ecosystems. These microorganisms secrete enzymes with a wide range of activity at low temperature, which can be used in various fields of the biotechnology industry.
Methods. To study the psychrotolerant ability of Bacillus mojavensis PS17 bacteria, a bacterial suspension prepared from a nocturnal culture was used. The ability of Bacillus mojavensis PS17 to grow at low temperatures was studied by kinetic measurement of optical density (OD) at a wavelength (l) of 595 nm. For this purpose, bacterial suspension of Bacillus mojavensis PS17 was inoculated into a basal medium and incubated at various low temperatures (5, 8, and 12 ± 1 °C) for 12 hours. The growth curve was measured every hour using a spectrophotometer. The activity of exogenous enzymes was determined by inoculation and incubation at a temperature of 4 ± 1 °C a Bacillus mojavensis PS17 bacterial suspension on a basal medium amended with 1% of different substrates such as milk powder, Tween-80 and sodium carboxymethylcellulose.
Results. The results showed that Bacillus mojavensis PS17 can grow at low temperatures. Evaluation of the activity of exogenous enzymes showed that the isolated enzymes of the studied strain do not lose their properties in conditions of low temperatures. The studied psychrotolerant properties of Bacillus mojavensis PS17 bacteria can be used in various manufacturing biotechnology such as food, textile and pharmaceutical.
Keywords
psychrotolerant bacteria,
exogenous enzymes activity,
phytase,
protease,
amylase,
cellulase,
Bacillus mojavensis PS17
Supporting agencies: The study was carried out with the financial support of the Ministry of Education and Science of the Russian Federation: agreement No. 075-15-2021-1395 dated October 25, 2022, unique contract identifier: RF----1930.61321X0001, project number: 15.IP.21. 0020.
About the Authors
R. G.C. Diabankana
Federal Research Center "Kazan Scientific Center of the Russian Academy of Sciences";
Center for Agroecological Research, Kazan State Agrarian University
Russian Federation
Russian Federation
associated researcher, laboratory of Molecular Genetic and Microbiological Methods, 2/31 Lobachevsky str., Kazan, 2/31. ул. Лобачевского, Казань, 420111;
associated researcher, 65, Karl Marx str., Kazan, 420111
A. N. Chernov
Tatar Research Institute of Agrochemistry and Soil Science
Russian Federation
Russian Federation
doctor of Biological Sciences, Head of the Department of Animal Husbandry and Veterinary Medicine, Chief Researcher, 2/31, Lobachevsky str., Kazan
Sh. Z. Validov
Federal Research Center "Kazan Scientific Center of the Russian Academy of Sciences"
Russian Federation
Russian Federation
doctor of Biological Sciences, Head of the laboratory of Molecular Genetics and Microbiological Methods, 2/31, Lobachevsky str., Kazan, 420111
D. M. Afordoanyi
Federal Research Center "Kazan Scientific Center of the Russian Academy of Sciences";
Tatar Research Institute of Agrochemistry and Soil Science
Russian Federation
Russian Federation
candidate of Biological Sciences, researcher, 2/31 Lobachevsky str., Kazan, 420111
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Review
For citations:
Diabankana R.G., Chernov A.N., Validov Sh.Z., Afordoanyi D.M. The ability of Bacillus mojavensis PS17 to grow and synthesize exogenous enzymes at low temperatures. Agrarian science. 2022;(9):143-146. (In Russ.) https://doi.org/10.32634/0869-8155-2022-362-9-143-146
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