Lonicera caerulea L.: Сравнительное метаболомное исследование полифенольного состава методом тандемной масс-спектрометрии четырех сортов дальневосточной жимолости

Актуальность. В статье приведено сравнительное метаболомное исследование ягод жимолости синей (L. caerulea), сортообразцы Волхова, Томичка, Голубое веретено, Амфора, представленные в коллекции Дальневосточной опытной станции ВИР.

Методы. Для определения комплекса вторичных метаболитов в экстрактах ягод жимолости синей использовали метод тандемной масс-спектрометрии. Для получения высококонцентрированных экстрактов применяли метод фракционной мацерации. В экстрактах ягод L. caerulea методом тандемной масс-спектрометрии достоверно идентифицировано 75 соединений полифенольной группы.

Результаты. По результатам качественной идентификации компонентного состава экстрактов ягод жимолости синей построена диаграмма Венна, показывающая сходство и различие по полифенольным группам различных сортообразцов L. caerulea. Ягоды L. caerulea содержат много биологически активных веществ, в частности имеют большое количество соединений с антиоксидантной активностью. Выделенный полифенольный комплекс включал в себя соединения следующих групп: флавоны, флавонолы, флаван-3-олы, флаваноны, антоцианы, стильбены, кумарины, фенольные кислоты. Впервые в экстрактах ягод L. caerulea идентифицировано 30 химических соединений полифенольной группы (флавоны пентагидроксидиметоксифлавон, цирсилиол, яцеозидин, софораизофлавон А, дигидрокситетраметоксифлавон, хризоэриол-О-гексозид, формононетин-7-О-глюкозид, флаван-3-олы эпиафзелеxин, эпикатехин галлат, стильбены пиносильвин, ресвератрол, дигидроресвератрол, кумарины фраксетин, умбеллиферон и др.), не упоминавшихся ранее в исследованиях по L. caerulea.

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