Preview

Agrarian science

Advanced search

Properties and application of betalaine dye isolated from regional beet variety

https://doi.org/10.32634/0869-8155-2023-374-9-185-190

Abstract

Relevance. Consumers in todayʼs society are concerned about the safety of using synthetic dyes to color food. The development and use in the food industry of natural dyes compositions based on available plant materials remains relevant. The use of natural dye based on betalains — pigments of beets (Beta vulgaris) — in the production of food products, allows not only to improve the color range of products, but also to enrich them with biologically active compounds. The objects of the study are beetroot pigments betalaines, concentrated natural dye and cream «Glace» with the addition of concentrated betalain dye.

Results. Depending on the experimental conditions, betalains were extracted from plant materials. The organoleptic and physico-chemical parameters of the concentrated natural dye and cream «Glasse» with its addition were studied.

Results. It has been shown that the optimal conditions for isolating betalains from beets are their extraction with 95% ethanol, ultrasonic extraction at 25 ºC, and extraction with an aqueous solution at 60 ºC for 30 min. It has been determined that the most stable color of the pigment is maintained in the range of pH = 3.7–8.2. In the region of pH > 10 there is a sharp change of the color. Betalain extracts at a pH > 10 acquire a rich dark red color. It was shown that the betalain dye obtained by the concentration method is a dark red liquid, which has antiradical activity (1,49 мМ) and contains 11 mg/cm3 of betalains. The best organoleptic characteristics were found in the cream «Glasse» with the addition of 5% concentrated betalain dye.

About the Authors

N. Yu. Chesnokova
Far Eastern Federal University
Russian Federation

Natalia Yuryevna Chesnokova, Doctor of Technical Sciences, Associate Professor

10 Ajax Bay, Russky Island, Vladivostok, 690922



A. A. Kuznetsova
Far Eastern Federal University
Russian Federation

Alla Alekseevna Kuznetsova, Candidate of Technical Sciences, Associate Professor

10 Ajax Bay, Russky Island, Vladivostok, 690922



L. V. Levochkina
Far Eastern Federal University
Russian Federation

Ludmila Vladimirovna Levochkina, Candidate of Technical Sciences, Associate Professor

10 Ajax Bay, Russky Island, Vladivostok, 690922



M. A. Tarabaev
Far Eastern Federal University
Russian Federation

Maksim Andreevich Tarabaev, Вachelor

10 Ajax Bay, Russky Island, Vladivostok, 690922



References

1. Chhikara N., Kushwaha K., Sharma P., Gat Yu., Panghal A. Bioactive compounds of beetroot and utilization in food processing industry: A critical review. Food Chemistry. 2019; 272: 192–200. https://doi.org/10.1016/j.foodchem.2018.08.022

2. Mella C., Vega-Gálvez A., Uribe E., Pasten A., Mejias N., Quispe-Fuentes I. Impact of vacuum drying on drying characteristics and functional properties of beetroot (Beta vulgaris). Applied Food Research. 2022; 2(1): 100120. https://doi.org/10.1016/j.afres.2022.100120

3. Seremet (Ceclu) L. et. al. Development of several hybrid drying methods used to obtain red beetroot powder. Food Chemistry. 2020; 310: 125637. https://doi.org/10.1016/j.foodchem.2019.125637

4. Gandía-Herrero F., García-Carmona F. Biosynthesis of betalains: yellow and violet plant pigments. Trends in Plant Science. 2013; 18(6): 334–343. https://doi.org/10.1016/j.tplants.2013.01.003

5. Polturak G., Aharoni A. «La Vie en Rose»: Biosynthesis, Sources, and Applications of Betalain Pigments. Molecular Plant. 2018; 11(1): 7–22. https://doi.org/10.1016/j.molp.2017.10.008

6. Poltorak G. et. al. Transcriptome and Metabolic Profiling Provides Insights into Betalain Biosynthesis and Evolution in Mirabilis jalapa. Molecular Plant. 2018; 11(1): 189–204. https://doi.org/10.1016/j.molp.2017.12.002

7. Gandía-Herrero F., Escribano J., García-Carmona F. Biological Activities of Plant Pigments Betalains. Critical Reviews in Food Science and Nutrition. 2016; 56(6): 937–945. https://doi.org/10.1080/10408398.2012.740103

8. Savitsky T., Wichkowski W. The effect of boiling and fermentation on betalain profiles and antioxidant capacity of red beet products. Food Chemistry. 2018; 259: 292–303. https://doi.org/10.1016/j.foodchem.2018.03.143

9. Figiel A. Drying kinetics and quality of beetroots dehydrated by combination of convective and vacuum-microwave methods. Journal of Food Engineering. 2010; 98(4): 461–470. https://doi.org/10.1016/j.jfoodeng.2010.01.029

10. Kazimierczak R. et. al. Beetroot (Beta vulgaris L.) and naturally fermented beetroot juices from organic and conventional production: metabolomics, antioxidant levels and anticancer activity. Journal of the Science of Food and Agriculture. 2014; 94(13): 2618–2629..https://doi.org/10.1002/jsfa.6722

11. Ustinova Yu.V., Ermolaeva E.O., Shevchenko T.V., Popov A.M., Plotnikov K.B. Properties and applications of natural betalain dyes. Technologies of the Food and Processing Industry of the Agro-Industrial Complex — Healthy Food Products. 2021; (4): 72–79 (In Russian). https://doi.org/10.24412/2311-6447-2021-4-72-79

12. Calva-Estrada S.J., Jiménez-Fernández M., Lugo-Cervantes E. Betalains and their applications in food: The current state of processing, stability and future opportunities in the industry. Food Chemistry: Molecular Sciences. 2022; 4: 100089. https://doi.org/10.1016/j.fochms.2022.100089

13. Buijnsters M., Bicanic D., Chirtoc M., Nicoli M.C., Min-Kuo Y. Evaluation of Antioxidative Activity of Some Antioxidants by Means of a Combined. Optothermal Window and a DPPH Free Radical Colorimetry. Analytical Sciences/Supplements. 2001; 17(S): s544–s546. https://doi.org/10.14891/ANALSCISP.17ICPP.0.S544.0

14. Kamiloglu S., Pasli A.A., Ozcelik B., Van Camp J., Capanoglu E. Colour retention, anthocyanin stability and antioxidant capacity in black carrot (Daucus carota) jan and marmalades: Effect of processing storage conditions and in vitro gastrointestinal digestion. Journal of Functional Foods. 2015; 13; 1–10. https://doi.org/10.1016/j.jff.2014.12.021

15. Jampani C., Raghavarao K.S.M.S. Process integration for purification and concentration of red cabbage (Brassica oleracea L.) anthocyanins Separation and Purification Technology. 2015; 141: 10–16. https://doi.org/10.1016/j.seppur.2014.11.024

16. Valdés A., Vidal L., Beltrán A., Canals A., Garrigós M.C. Microwave-Assisted Extraction of Phenolic Compounds from Almond Skin Byproducts (Prunus amygdalus): A Multivariate Analysis Approach. Journal of Agricultural and Food Chemistry. 2015; 63(22): 5395–5402. https://doi.org/10.1021/acs.jafc.5b01011

17. Minzanova S.T. et. al. Phytogenous betalaine pigments: isolation, structure and chemical properties. Butlerov Communications. 2010; 19(2): 1–11. (In Russian). https://www.elibrary.ru/ncylcr

18. Mikołajczyk-Bator K., Pawlak S. The effect of the thermal treatment on antioxidant capacity and pigment contents in separated betalain fractions. Acta Scientiarum Polonorum Technologia Alimentaria. 2016; 15(3): 257–265. https://doi.org/10.17306/J.AFS.2016.3.25


Review

For citations:


Chesnokova N.Yu., Kuznetsova A.A., Levochkina L.V., Tarabaev M.A. Properties and application of betalaine dye isolated from regional beet variety. Agrarian science. 2023;(9):185-190. (In Russ.) https://doi.org/10.32634/0869-8155-2023-374-9-185-190

Views: 521


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 0869-8155 (Print)
ISSN 2686-701X (Online)
X