Dynamics of extraction of minerals from the feed substrate in vitro with including low molecular weight additives into rumen reaction medium

The ban on the use of antibiotics for therapeutic purposes encourages the search for highly effective alternatives, one of which is phytobiotics. However, before their large-scale implementation into practice, it is necessary to conduct a detailed assessment of the impact on various aspects of life.

The aim of the work was to study the dynamics of the elemental profile of the ruminant rumen in vitro when vanillin, transcoric aldehyde, dihydroxyquercetin and 7-hydroxycoumarin were introduced into feed.

The study was conducted on a single-quadrupole inductively coupled plasma mass spectrometer Agilent 7900 ICP-MS (Agilent, USA). The analysis of elements such as Cr, Fe and Zn was carried out in the helium mode using a collision cell. During the work, it was found that vanillin in concentrations from 1,225 × 10^-4 to 4,900 × 10^-4 mol/l reduces the accumulation of copper, cobalt and lead, stimulating the extraction of manganese, iron, aluminum and barium. Quercetin and cinnamic aldehyde, on the contrary, inhibit the extraction of almost all the studied elements. Coumarin, on the other hand, contributes more to the extraction of minerals from the cellulose matrix of plant components of the feed, with the exception of copper, cobalt and zinc. At the same time, the best effect is detected by a combination of quercetin and vanillin at concentrations of 2,450 × 10^-4 and 1,225 × 10^-4 mol/l, respectively. All the studied substances demonstrate the potential for the correction of hypo- and hyperelementoses of various types.

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