Molecular assessment of genetic homogeneity of flax lines using SSR-analysis

The problem of genetic purity of agricultural crop varieties is one of the key issues in the field of agricultural biology and its importance in the agricultural sector of the economy is constantly increasing. The uniformity of the genotypic and phenotypic characteristics of plants within the variety makes it possible to preserve its unique properties from generation to generation. However, due to the influence of many factors from natural mutagenesis, gene drift, over-pollination and violations of seed production technologies, the purity of the variety can decrease significantly. The introduction of molecular genetic labeling technologies into seed breeding practice will contribute to an effective and rapid assessment of the genetic purity of varieties.

The purpose of this work is to evaluate the genetic uniformity of flax lines using various SSR marker complexes.

The objects of the study were 4 flax lines, each represented by 16 samples. Genomic DNA was isolated from plant leaves using a modified CTAB-method. SSR-marker complexes of domestic and foreign development were used in the work. After PCR was performed with each line of SSR-markers, a fragmentary analysis was performed, based on which the genetic uniformity of the studied flax lines was evaluated and compared. Data analysis showed that all the lines studied were heterogeneous in composition to varying degrees, with the M-326 line being the most variable and the P-268 line being the least variable. Both marker complexes used showed high information content and complementarity of the data obtained. Based on the results of the research, the most promising SSR-markers have been identified, the further use of which will ensure the best results and accuracy in data interpretation. 

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