Сравнительная эффективность протоколов синхронизации овуляции с прогестероновым имплантатом у тёлок симментальской породы

Актуальность. Целью данной работы являлась синхронизация половой охоты у симментальских телок определенного возраста в хозяйстве, занимающемся продажей стельных нетелей. Для этого проводили введение прогестерона с разными временны́ми интервалами — как у циклических, так и у ациклических животных — с последующей оценкой эффективности примененных протоколов синхронизации.

Методика. В исследовании использовали сто голов симментальских телок. Из общего поголовья методом случайной выборки сформировали пять равных групп. Контрольная группа (n = 20): наблюдение за признаками охоты без какого-либо гормонального воздействия и трехкратное осеменение. Группа 1 (n = 20): на 0-й день — имплантат ГнРГ + прогестерон; на 5-й день — удаление имплантата, введение PGF2α + прогестерон; на 8-й день — ГнРГ + осеменение. Группа 2 (n = 20): 0-й день — имплантат ГнРГ + прогестерон; 5-й день — удаление имплантата, PGF2α + прогестерон; 8-й день — ГнРГ + первое осеменение и 9-й день — повторное осеменение. Группа 3 (n = 20): 0-й день — имплантат ГнРГ + прогестерон; 8-й день — удаление имплантата, PGF2α + прогестерон; 11-й день — ГнРГ + осеменение. Группа 4 (n = 20): 0-й день — имплантат ГнРГ + прогестерон; 8-й день — удаление имплантата, PGF2α + прогестерон; 11-й день — ГнРГ + первое осеменение и 12-й день — ГнРГ + повторное осеменение.

Результаты. Частота наступления беременности в группах составила 30, 65, 80, 30 и 15% соответственно. Самый высокий показатель был зарегистрирован во второй группе. Установлено, что именно эту процедуру следует рекомендовать всем фермерским хозяйствам, заинтересованным в получении стельных тёлок.

1. Eshete T., Demisse T., Yilma T., Tamir B. Repeat Breeding and Its’ Associated Risk Factors in Crossbred Dairy Cattle in Northern Central Highlands of Ethiopia. Veterinary Medicine International. 2023; 2023: 1176924.https://doi.org/10.1155/2023/1176924

2. Xu Z.Z., Burton L.J. Estrus Synchronization of Lactating Dairy Cows with GnRH, Progesterone, and Prostaglandin F2α. Journal of Dairy Science. 2000; 83(3): 471–476.https://doi.org/10.3168/jds.S0022-0302(00)74905-8

3. Kolachi H.A. et al. Fixed-time artificial insemination technology in buffaloes: a review. Frontiers in Veterinary Science. 2025; 12: 1586609.https://doi.org/10.3389/fvets.2025.1586609

4. Coffman E.A., Pinto C.R. A Review on the Use of Prostaglandin F2α for Controlling the Estrous Cycle in Mares. Journal of Equine Veterinary Science. 2016; 40: 34–40.https://doi.org/10.1016/j.jevs.2016.01.008

5. Liehr R.A., Marion G.B., Olson H.H. Effects of prostaglandin on cattle estrous cycles. Journal of Animal Science. 1972; 35(S1).

6. López-Gatius F. Ovarian response to prostaglandin F2α in lactating dairy cows: A clinical update. Journal of Reproduction and Development. 2022; 68(2): 104–109.https://doi.org/10.1262/jrd.2021-119

7. Fortune J.E., Sirois J., Quirk S.M. The growth and differentiation of ovarian follicles during the bovine estrous cycle. Theriogenology. 1988; 29(1): 95–109.https://doi.org/10.1016/0093-691X(88)90034-9

8. Sartori R., Fricke P.M., Ferreira J.C.P., Ginther O.J., Wiltbank M.C. Follicular Deviation and Acquisition of Ovulatory Capacity in Bovine Follicles. Biology of Reproduction. 2001; 65(5): 1403–1409.https://doi.org/10.1095/biolreprod65.5.1403

9. Twagiramungu H., Guilbault L.A., Dufour J.J. Synchronization of ovarian follicular waves with a gonadotropin-releasing hormone agonist to increase the precision of estrus in cattle: A review. Journal of Animal Science. 1995; 73(10): 3141–3151.https://doi.org/10.2527/1995.73103141x

10. Thatcher W.W. et al. New clinical uses of GnRH and its analogues in cattle. Animal Reproduction Science. 1993; 33(1–4): 27–49.https://doi.org/10.1016/0378-4320(93)90105-Z

11. Kojima F.N. et al. Development of an estrus synchronization protocol for beef cattle with short-term feeding of melengestrol acetate: 7-11 synch. Journal of Animal Science. 2000; 78(8): 2186–2191.https://doi.org/10.2527/2000.7882186x

12. Wood-Follis S.L., Kojima F.N., Lucy M.C., Smith M.F., Patterson D.J. Estrus synchronization in beef heifers with progestin-based protocols. I. Differences in response based on pubertal status at the initiation of treatment. Theriogenology. 2004; 62(8): 1518–1528.https://doi.org/10.1016/j.theriogenology.2004.02.017

13. do Couto S.R.B., Silenciato L.N., Okada M. dos S.D., e Silva O.R., Ferreira J.E., de Mello M.R.B. Use of Progesterone as a Strategy to Improve Reproductive Efficiency in Cattle. Wang Z. (ed.). Progesterone - Basic Concepts And Emerging New Applications. IntechOpen. 2024.https://doi.org/10.5772/intechopen.1005624

14. Salverson R.R., DeJarnette J.M., Marshall C.E., Wallace R.A. Synchronization of estrus in virgin beef heifers using melengestrol acetate and PGF2α: an efficacy comparison of cloprostenol and dinoprost tromethamine. Theriogenology. 2002; 57(2): 853–858.https://doi.org/10.1016/s0093-691x(01)00692-6

15. Patterson D.J., Kojima F.N., Smith M.F. A review of methods to synchronize estrus in replacement heifers and postpartum beef cows. Journal of Animal Science. 2003; 81(14-S2): E166–E177.https://doi.org/10.2527/2003.8114_suppl_2E166x

16. Lamb G.C., Nix D.W., Stevenson J.S., Corah L.R. Prolonging the MGA-prostaglandin F2α interval from 17 to 19 days in an estrus synchronization system for heifers. Theriogenology. 2000; 53(3): 691–698.https://doi.org/10.1016/S0093-691X(99)00267-8

17. Wood S.L., Lucy M.C., Smith M.F., Patterson D.J. Improved synchrony of estrus and ovulation with the addition of GnRH to a melengestrol acetate-prostaglandin F2α synchronization treatment in beef heifers. Journal of Animal Science. 2001; 79(8): 2210–2216.https://doi.org/10.2527/2001.7982210x

18. Lucy M.C. et al. Efficacy of an intravaginal progesterone insert and an injection of PGF2α for synchronizing estrus and shortening the interval to pregnancy in postpartum beef cows, peripubertal beef heifers, and dairy heifers. Journal of Animal Science. 2001; 79(4): 982–995.https://doi.org/10.2527/2001.794982x

19. Kara U., Bekyürek T. The Effect of Double PGF2α Administrations Applıed to Donor Before Oestrus With Synchronized CIDR on the Number and Qualıty of Obtained Embryos During Embryo Transfer in Cattle. Sağlık Bilimleri Dergisi. 2017; 26(3): 204–210 (in Turkish).

20. Utter S.D., Corah L.R. Influence of dietary energy levels on reproductive function and fertility in yearling beef heifers. Kansas Agricultural Experiment Station Research Reports. 1994; (1).https://doi.org/10.4148/2378-5977.2074

21. Lamb G.C. et al. Synchronization of estrus and artificial insemination in replacement beef heifers using gonadotropinreleasing hormone, prostaglandin F2α, and progesterone. Journal of Animal Science. 2006; 84(11): 3000–3009.https://doi.org/10.2527/jas.2006-220

22. Busch D.C. et al. Comparison of progestin-based estrus synchronization protocols before fixed-time artificial insemination on pregnancy rate in beef heifers. Journal of Animal Science. 2007; 85(8): 1933–1939.https://doi.org/10.2527/jas.2006-845

23. Leitman N.R. et al. Comparison of controlled internal drug release insert-based protocols to synchronize estrus in prepubertal and estrous-cycling beef heifers. Journal of Animal Science. 2009; 87(12): 3976–3982.https://doi.org/10.2527/jas.2009-2250

24. Coman S., Berean D.I., Cimpean R., Ciupe S., Coman I., Bogdan L.M. Clinical Modalities for Enhancing Reproductive Efficiency in Buffaloes: A Review and Practical Aspects for Veterinary Practitioners. Animals. 2024; 14(18): 2642.https://doi.org/10.3390/ani14182642

25. Pursley J.R., Silcox R.W., Wiltbank M.C. Effect of Time of Artificial Insemination on Pregnancy Rates, Calving Rates, Pregnancy Loss, and Gender Ratio After Synchronization of Ovulation in Lactating Dairy Cows. Journal of Dairy Science. 1998; 81(8): 2139–2144.https://doi.org/10.3168/jds.s0022-0302(98)75790-x

26. Lamb G.C., Cartmill J.A., Stevenson J.S. Effectiveness of Select Synch (Gonadotropin-Releasing Hormone and Prostaglandin F2α) for Synchronizing Estrus in Replacement Beef Heifers. The Professional Animal Scientist. 2004; 20(1): 27–33.https://doi.org/10.15232/S1080-7446(15)31268-7

27. Bader J.F. et al. A comparison of progestin-based protocols to synchronize ovulation and facilitate fixed-time artificial insemination in postpartum beef cows. Journal of Animal Science. 2005; 83(1): 136–143.https://doi.org/10.2527/2005.831136x

28. Day M.L., Grum D.E. Breeding Strategies to Optimize Reproductive Efficiency in Beef Herds. Veterinary Clinics of North America: Food Animal Practice. 2005; 21(2): 367–381.https://doi.org/10.1016/j.cvfa.2005.02.003

29. Kasimanickam R., Harting Q., Hanson R., Boler R. Estrus and ovulation synchronization strategies in beef cattle. Clinical Theriogenology. 2025; 17(Therapeutics in Theriogenology): 9–23.https://doi.org/10.58292/CT.v17.11650

30. Amer, P. R., Lowman, B. G., & Simm, G. Economic values for reproduction traits in beef suckler herds based on a calving distribution model. Livestock Production Science, 1996; 46(2), 85–96. https://doi.org/10.1016/0301-6226(96)00016-4

31. Cavalieri J., Kinder J.E., De’ath G., Fitzpatrick L.A. Effect of 48 h treatment with 17β-oestradiol or progesterone on follicular wave emergence and synchrony of ovulation in Bos indicus cows when administered at the end of a period of progesterone treatment. Animal Reproduction Science. 1997; 46(3–4): 187–201.https://doi.org/10.1016/s0378-4320(96)01620-x

32. Arköse C.Ç., Uslu B.A. Comparison of the Results Synchronization with Progesterone Implants and Fixed Time Artificial Insemination in Dairy Cows. Brazilian Archives of Biology and Technology. 2022; 65: e22210243.https://doi.org/10.1590/1678-4324-2022210243

33. Stevenson, J. S., Pursley, J. R., Garverick, H. A., Fricke, P. M., Kesler, D. J., Ottobre, J. S., & Wiltbank, M. C. Treatment of cycling and noncycling lactating dairy cows with progesterone during Ovsynch. Journal of Dairy Science, 2006; 89(7), 2567–2578. https://doi.org/10.3168/jds.S0022-0302(06)72333-5

34. Martínez M.F., Kastelic J.P., Adams G.P., Janzen E., McCartney D.H., Mapletoft R.J. Estrus synchronization and pregnancy rates in beef cattle given CIDR-B, prostaglandin and estradiol, or GnRH. The Canadian Veterinary Journal. 2000; 41(10): 786–790.

35. Dinç D.A. İneklerde reprodüktif verimliği artırma programları. Veteriner Hekimler Derneği Dergisi. 2006; 77(2): 50–64 (in Turkish).

36. Fricke P.M. Scanning the Future—Ultrasonography as a Reproductive Management Tool for Dairy Cattle. Journal of Dairy Science. 2002; 85(8): 1918–1926.https://doi.org/10.3168/jds.S0022-0302(02)74268-9

37. Stevenson J.S., Tiffany S.M. Resynchronizing Estrus and Ovulation After Not-Pregnant Diagnosis and Various Ovarian States Including Cysts. Journal of Dairy Science. 2004; 87(11): 3658–3664.https://doi.org/10.3168/jds.S0022-0302(04)73504-3

38. Vasconcelos J.L.M., Silcox R.W., Rosa G.J., Pursley J.R., Wiltbank M.C. Synchronization rate, size of the ovulatory follicle, and pregnancy rate after synchronization of ovulation beginning on different days of the estrous cycle in lactating dairy cows. Theriogenology. 1999; 52(6): 1067–1078.https://doi.org/10.1016/S0093-691X(99)00195-8

39. Doležel R., Čech S., Zajíc J., Havlíček V. Oestrus Synchronization by PGF2α and GnRH in Intervals according to Stage of Follicular Development at Time of Initial Treatment in Cows. Acta Veterinaria Brno. 2002; 71(1): 101–108.https://doi.org/10.2754/avb200271010101

40. Pursley J.R., Wiltbank M.C., Stevenson J.S., Ottobre J.S., Garverick H.A., Anderson L.L. Pregnancy Rates Per Artificial Insemination for Cows and Heifers Inseminated at a Synchronized Ovulation or Synchronized Estrus. Journal of Dairy Science. 1997; 80(2): 295–300.https://doi.org/10.3168/jds.S0022-0302(97)75937-X

41. Stevenson J.S., Kobayashi Y., Thompson K.E. Reproductive Performance of Dairy Cows in Various Programmed Breeding Systems Including OvSynch and Combinations of GonadotropinReleasing Hormone and Prostaglandin F2a. Journal of Dairy Science. 1999; 82(3): 506–515.https://doi.org/10.3168/jds.S0022-0302(99)75261-6

42. Nak Y. et al. Siklik ve Asiklik Anöstruslu Sütçü Düvelerde Ovsynch veya PRID + PGF2 + PMSG Uygulamalarının Reproduktif Performans Üzerine Etkileri. Uludağ Üniversitesi Veteriner Fakültesi Dergisi. 2005; 24(1–4): 21–26.

43. Thatcher W.W., Bartolome J.A., Sozzi A., Silvestre F., Santos J.E.P. Manipulation of Ovarian Function for the Reproductive Management of Dairy Cows. Veterinary Research Communications. 2004; 28(S1): 111–119.https://doi.org/10.1023/b:verc.0000045388.07807.3e

44. Wiltbank M.C., Souza A.H., Carvalho P.D., Bender R.W., Nascimento A.B. Improving fertility to timed artificial insemination by manipulation of circulating progesterone concentrations in lactating dairy cattle. Reproduction, Fertility, and Development. 2011; 24(1): 238–243.https://doi.org/10.1071/RD11913

45. Bisinotto R.S., Ribeiro E.S., Santos J.E.P. Synchronisation of ovulation for management of reproduction in dairy cows. Animal. 2014; 8(S1): 151–159.https://doi.org/10.1017/S1751731114000858

46. Martínez M.F., Kastelic J.P., Adams G.P., Cook B., Olson W.O., Mapletoft R.J. The use of progestins in regimens for fixed-time artificial insemination in beef cattle. Theriogenology. 2002; 57(3): 1049–1059.https://doi.org/10.1016/s0093-691x(01)00682-3

47. Rabiee A.R., Lean I.J., Stevenson M.A. Efficacy of Ovsynch Program on Reproductive Performance in Dairy Cattle: A Meta-Analysis. Journal of Dairy Science. 2005; 88(8): 2754–2770.https://doi.org/10.3168/jds.S0022-0302(05)72955-6

48. Bülbül B., Ataman M.B. Investigation of Effect on Fertility of Different Oestrus Synchronization Methods in Cows in Field Conditions. Eurasian Journal of Veterinary Sciences. 2005; 21(3–4): 15–22 (in Turkish).

49. Pursley J.R., Mee M.O., Wiltbank M.C. Synchronization of ovulation in dairy cows using PGF2α and GnRH. Theriogenology. 1995; 44(7): 915–923.https://doi.org/10.1016/0093-691x(95)00279-h

50. Khalil A.A.Y. Fertility response of lactating dairy cows subjected to three different breeding programs under subtropical conditions. Beni-Suef University Journal of Basic and Applied Sciences. 2019; 8: 6.https://doi.org/10.1186/s43088-019-0008-x