The effectiveness of antibiotics in the recovery of microclones rootstocks MaxMa 14, GF 677, Myrobalan 29C from infections of various etiologies

Abstract

The article presents experimental data on the effectiveness of various groups of antibiotics in the rehabilitation of the explants of some rootstocks of stone fruit crops from infections of various etiologies brought from the environment, in violation of the rules of sterility in working with an in vitro culture. The results of the study showed that the morphogenetic response of explants of clonal rootstocks to antibiotics depends on their varietal origin. Thus, a greater number of the introduced explants Myrobalan 29C necrotic (25-100%). The number of normally developed microplants for different rootstocks ranges from 0 to 75. It was found that all studied antibiotic concentrations, except ceftriaxone 10 mg/l and nystatin 100 mg/l, positively affect the viability of MaxMa 14. For rootstocks Myrobalan 29C maximum the number of viable explants (60% of healthy explants) showed the environment with the antibiotic nystatin at a concentration of 200 mg/l. For rootstocks GF 677, the maximum number of well-developed micro shoots was observed on nutrient media with antibiotics tetracycline at a concentration of 100 mg/l and ceftriaxone at a concentration of 200 mg/l (60% of healthy explants in both variants).