Comparative analysis of classical laboratory methods for isolation of representatives of the genus Phytophthora from soil and plants

The article describes laboratory methods for isolating members of the Phytophthora genus from soil and plant tissues (bio baits, inoculation of medium, flotation, etc.). Currently, of great interest is the issue of preventing the development of root rot of fruit and berry crops, among the causes of which are the activity of soil micromycetes. The experimental material was used to analyze the possibility of using classical methods of identifying late blight pathogens (Phytophthora oomycetes) in the soil and plant elements (fruit plantations) in routine examinations. It has been established that the bio bait method has a certain advantage over the seeding method. Using bio baits, it is possible to analyze a large volume of soil, in contrast to the method of planting on solid nutrient medium, where a small amount of soil can be placed in the volume of a Petri dish. But the flotation method allows you to get results faster than the bait method. We suggest using an integrated approach for solving phytopathological problems when examining fruit and berry crops, combining the method of flotation and / or bait with apple slices and planting on specialized nutrient media (tomato or carrot agar).

фитофтора, корневые гнили, диагностика, почва, растение, флотация, метод приманок, питательная среда, томатный агар, плодовые культуры, Phytophthora, root rot, diagnostics, soil, flotation, bait method, inoculation of medium, tomato agar, fruit