Use of pgpr bacteria for protection sugar beet from root rot

Abstract

In the recent years, the use of soil microorganisms has generated great interests in crop production by reducing the damage of diseases. Actualite.The application of PGPR bacteria makes possible to improve the quantity and quality of plant s harvest and to reduce chemical pressure on the environment. The aim of investigations - study of the ability of the soil saprophytic diazotrophs bacteria (PGPR) reducing the infectious background of root rot diseases. deposited in the National Collection of nonpathogenic microorganisms of Moldova - Peudomonas aureofaciens CNMN-PsB-05, Pseudomonas fluorescens CNMN-PsB-04, Pseudomonas putida-PsB-06, Bacillus subtilis CNMN-bB-08. Materials. The agents pathogens from the beet tuber were isolated on potato-glucose agar and were identification as Fusarium oxysporum and Fusarium gibbosum, They reduce the shoots of beet seeds for 60-75%. The ability for antagonism strains Peudomonas aureofaciens CNMN-PsB-05, Pseudomonas fluorescensCNMN-PsB-04, Pseudomonas putida - PsB- 06, Bacillus subtilis CNmN-BB-08, deposited in the National Collection of nonpathogenic microorganisms of Moldova was defined in laboratory tests. Results. The suspension of bacterial strains with titre 108 CFU/ml show the antagonism in vitro toward the fitopathogens in time cultivation combine in Petri plates: the radius of delay growth Fusarium spp. were establish. Traitment of beet seeds, inoculate with pathogens, in time sowing with bacterials suspensions decreased the level of infection from agents pathogens of root rot, increased the shoots of beet seeds for 20-30%, the weight of tuber in 2.2 4,0 one and improved production of plants.