First results of assessment potato varieties of Kazakhstan breeding on the availability of resistance genes to potato virus Y with the help of molecular markers

The goal of the research was to investigate potato varieties of Kazakhstan breeding for the presence of DNA markers linked to genes of extreme resistance to potato virus Y (PVY), as well as to determine the level of resistance of potato samples to PVY. The object of the researnh was the biomaterial of eleven potato varieties created in the North-West Scientific and Production Center of Agriculture, Republic of Kazakhstan. Using the polymerase chain reaction, DNA marker RYSC3 (Ry-and gene) was detected in Vid-1, Kostanaiskye novosti and Kurant-1. The RAPD marker PVY38-530 (gene PVY38-530) was detected in the genome of the Alaya zarya-2 and Dunyasha samples. Markers PVY38-530 and Ry186 (Ry-chc gene) were found in the Udovitsky variety, and markers RYSC3 and PVY38-530 were found in the varieties Valery and Tustep. Molecular DNA markers designed to search for the Ry-fsto (GP122-406), Ry-sto (YES3-3A, STM003), and Ny-1 (S1d11) genes were not detected in all eleven studied samples. In the biomaterial varieties Akzhar, Alaya zarya and Vid-2, none of the seven diagnosable markers were detected. To determine the level of samples resistance of to PVY, artificial infection of plants with the virus was carried out in open ground conditions and under controlled conditions of the phytotron. The assessment was performed by visual observation of symptoms on inoculated potato plants, as well as using enzyme-linked immunosorbent assay (ELISA). The varieties Valery, Vid-1 and Kostanaiskye novosti are attributed as the samples with extreme resistance type. The susceptible samples include Akzhar, Alaya zarya, Duniasha and Kurant-1. Alaya zarya-2, Vid-2, Tustep and Udovitsky varieties were assigned as weakly susceptible specimens, on the plants a local necrotic reaction were found accompanied by a weakly positive level of the detected signal in the ELISA. The results can be used in breeding programs for the creation of virus-resistant potato varieties, as well as in basic research on the study of the mechanisms of potato resistance to PVY.

картофель, устойчивость, Y-вирус картофеля, ДНК-маркеры, полимеразная цепная реакция, иммуноферментный анализ, potato, resistance, potato virus X DNA markers, polymerase chain reaction, enzyme-linked immunosorbent assay