Development of monoclonal antibodies - based test systems for detection of potato viruses X and M

Abstract

Viral diseases cause serious damage to the potato, significantly reducing crop yields. Infection of a plant with a single virus leads to a yield loss of approximately 30%, and the simultaneous influence of two or more viruses can lead to almost complete loss of yield or even death of plants. The undoubted problem is a decrease in the immunity of infected plants and their increased tendency to infect fungal and microbial pathogens. In this regard, the control of infection of seed material is important. The presented work is part of a study on the development of a multiparameter potato virus detection system. Representative panels of monoclonal antibodies that recognize potato viruses X and M have been obtained. The possibility of using monoclonal antibodies to detect virus particles by immunoblotting has been shown. On the basis of the obtained antibodies, test systems were developed for the quantitative determination of potato viruses X and M with a linear range of PVX measurement of 0.4 - 50 ng /ml and PVM 6 - 400 ng /ml.