Study of the growth-promoting activity of plant polysaccharides of Gleditsia triacanthos, Crotalaria alata and Crotalaria sp.

Relevance. In plant growing practice plant growth regulators based on plant substances are widely used. Plant polysaccharides are among the biologically active compounds that can be used as natural raw materials to create new drugs.

Methods. The growth-promoting activity of galactomannans isolated from the seeds of the plants Crotalaria alata, Gleditsia triacanthos and Crotalaria sp. was studied on cotton and wheat crops when they were grown under saline conditions. The range of studied concentrations was in the range from 0.1% to 0.0001%. Presowing treatment was carried out for 18 hours, after which the seeds were germinated in Petri dishes with the addition of 1% NaCl saline solution at a temperature of 25 °C. The biological activity of polysaccharides was evaluated by the linear growth of aboveground and underground organs of seedlings. The results were statistically processed using the Original Pro program.

Results. The studied substances have growth-stimulating activity, which depended on their origin and concentration. So galactomannan obtained from Crotalaria alata, under salinization conditions, had a positive effect on the growth of wheat root at 0.01% concentration, and from Gleditsia triacanthos at lower concentrations, 0.001% and 0.0001%. On the stem growth, the maximum effect was exerted by a substance isolated from Crotalaria alata in 0.01% and 0.001% doses. On cotton, stem growth was activated by the polysaccharide Crotalaria sp. in 0.0001% concentrations, the root is galactomannan Gleditsia triacanthos in 0.01% concentration.

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