Analysis of spread of porcine circovirus type 3 detected in Russia
https://doi.org/10.32634/0869-8155-2020-342-10-28-30
Abstract
Porcine circovirus type 3 (PCV-3) — a recently detected member of Circoviridae family. The virus has been identified as a possible infectious agent. Clinical signs vary from stillbirth and fetus mummification, encephalitis and myocarditis in perinatal period to periarteritis in fattening period. The PCV-3 widespread has been demonstrated in major pork development countries. Establishing of the association between presence of this pathogen and disease development makes PCV3 diagnostic studies in Russia necessary. The main goal of this investigation was to detect PCV3 DNA in blood serum, saliva and bronchioalveolar lavage and pathological material in sows and piglets of different age groups at two units at an industrial swine farm. For PCV-3 detection we used PCR method.
Results of this investigation revealed that PCV3 was detected in all types of biomaterial. At the unit 1 PCV3 DNA has been detected in saliva (highes ratio of infected animals was 60% in 21- and 60–70 day-old piglets), serum (100% in 150–160-day-old piglets) and bronchioalveolar fluid (40% in 90–100-day-old piglets). It was found that at the unit 2 level of piglets PCV-3 shedding was higher (50–100%) than at the unit 1 (0–60%). Moreover, PCV3 DNA has been detected in 70% of pathological material obtained from aborted sows and in 80% of their amniotic fluid.
About the Authors
S. A. Raev
Federal State Budget Institution “Federal Scientific Center VIEV”
Russian Federation
Ph.D., Leading Researcher
109428, Ryazanskiy prospect 24-1, Moscow, Russia
Russian Federation
Ph.D., Leading Researcher
109428, Ryazanskiy prospect 24-1, Moscow, Russia
A. G. Yuzhakov
Federal State Budget Institution “Federal Scientific Center VIEV”
Russian Federation
Russian Federation
Candidate of Biological Sciences, Senior Researcher
109428, Ryazanskiy prospect 24-1, Moscow, Russia
V. V. Stafford
Federal State Budget Institution “Federal Scientific Center VIEV”
Russian Federation
Ph.D., Senior Researcher
109428, Ryazanskiy prospect 24-1, Moscow, Russia
Russian Federation
Ph.D., Senior Researcher
109428, Ryazanskiy prospect 24-1, Moscow, Russia
A. D. Zaberezhny
Federal State Budget Institution “Federal Scientific Center VIEV”
Russian Federation
Russian Federation
Doctor of Biological Sciences, Deputy Director for Science
109428, Ryazanskiy prospect 24-1, Moscow, Russia
T. I. Aliper
Federal State Budget Institution “Federal Scientific Center VIEV”
Russian Federation
Russian Federation
Doctor of Biological Sciences, Head of the Laboratory
109428, Ryazanskiy prospect 24-1, Moscow, Russia
References
1. Сучкова С.А., Лукашенко Е.В., Ермилов И.В. Цирковирус свиней 3-го типа: результаты скрининга в России. Свиноводство. 2019; 2: 69-70.
2. Орлянкин Б.Г., Раев С.А, Алипер Т.И. Новый цирковирус свиней: распространение и роль в патологии. Свиноводство. 2017; 5: 64-65.
3. Arruda B., Piñeyro P., Derscheid R. PCV3-associated disease in the United States swine herd. Emerg Microbes Infect. 2019; 8(1):684-698.
4. Collins P.J., McKillen J., Allan G. Porcine circovirus type 3 in the UK. Veterinary Record. 2017$ 181: 599.
5. Jiang H., Wang D., Wang J. Induction of Porcine Dermatitis and Nephropathy Syndrome in Piglets by Infection with Porcine Circovirus Type 3. J Virol. 2019; 93(4): 02045-18.
6. Kedkovid R., Woonwong Y., Arunorat J., et al. Porcine circovirus type 3 (PCV3) infection in grower pigs from a Thai farm suffering from porcine respiratory disease complex (PRDC). Veterinary Microbiology. 2018; 215: 71–76.
7. Kwon T., Yoo S.J., Park С.К., et al. Prevalence of novel porcine circovirus 3 in Korean pig populations. Veterinary Microbiology. 2017; 207:178-180.
8. Mora-Díaz J., Piñeyro P., Shen H., Schwartz K., Vannucci F., Li G., Arruda B., Giménez-Lirola L. Isolation of PCV3 from Perinatal and Reproductive Cases of PCV3-Associated Disease and In Vivo Characterization of PCV3 Replication in CD/CD Growing Pigs. Viruses. 2020 Feb 16;12(2):219.
9. Palinski R., Pineyro P., Shang P., et al. A novel porcine circovirus distantly related to known circoviruses is associated with porcine dermatitis and nephropathy syndrome and reproductive failure. Journal of Virology. 2016; 91(1): 01879-16.
10. Phan T. G., Giannitti F., Rossow S., et al. Detection of a novel circovirus PCV3 in pigs with cardiac and multi-systemic inflammation. Virology Journal. 2016; 13(1): 184.
11. Stadejek T, Wozniak A, Miłek D, et al. First detection of porcine circovirus type 3 on commercial pig farms in Poland. Transbound Emerg Dis. 2017; 64(5): 1350-1353.
12. Saporiti V, Martorell S, Cruz TF, et al. Frequency of Detection and Phylogenetic Analysis of Porcine circovirus3 (PCV-3) in Healthy Primiparous and Multiparous Sows and Their Mummified Fetuses and Stillborn. Pathogens. 2020;9(7):533.
13. Tochetto C., Lima D.A., Varela A.P.M., et al. Full-Genome Sequence of Porcine Circovirus type 3 recovered from serum of sows with stillbirths in Brazil. Transbound Emerg Dis. 2017; 65(1): 5–9.
14. Ye, X., Berg, M., Fossum, C. et al. Detection and genetic characterisation of porcine circovirus 3 from pigs in Sweden. Virus Genes. 2018; 54(3): 466-469.
15. Yuazhakov A.G., Raev S.A., Alekseev K.P., et al. First detection and full genome sequence of porcine circovirus type 3 in Russia. Virus Genes. 2018; 54 (4): 608-611.
16. Zheng S., Wu X., Zhang L., et al. The occurrence of porcine circovirus 3 without clinical infection signs in Shandong Province. Transbound Emerg Dis. 2017; 64(5): 1337–1441.
Review
For citations:
Raev S.A., Yuzhakov A.G., Stafford V.V., Zaberezhny A.D., Aliper T.I. Analysis of spread of porcine circovirus type 3 detected in Russia. Agrarian science. 2020;(10):28-30. (In Russ.) https://doi.org/10.32634/0869-8155-2020-342-10-28-30
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