Evaluation of the gene pool of winter bread wheat of the CIMMYT collection by the presence of the gene for resistance to leaf rust Lr10

Abstract

Relevance and methods. Due to the need for food security, the country needs selfsufficiency in grain, especially winter soft wheat. To obtain high grain yields, varieties of soft winter wheat that are resistant to major leaf diseases are required. Thus, the task of finding resistance genes for following to pyramiding them in one genotype  is urgent. This study aimed to assess the gene pool of soft winter wheat from the CIMMYT collection (Mexico) for the presence of the gene for resistance to leaf rust Lr10. The work used: CTAB-method (DNA extraction); PCR method (according to the developed protocols for determining resistance genes to the diseases of winter wheat); method of electrophoresis on agarose gels.

Results. The presence of a functional allele of the Lr10 gene was established in 76 samples of winter bread wheat out of 411 studied (sets 20th IWWYT-SA, 21th IWWYT-IRR, 25th FAWWON-IRR, 25th FAWWON-SA). We identified 15 samples with atypical alleles of the Lr10 gene. In 320 samples of winter wheat, there was no amplification of DNA fragments with the molecular marker Lrk10-D. It may indicate for samples genotype a significant variability of the microsatellite sequence, for which the molecular marker was made. The 76 samples of soft winter wheat from the CIMMYT collection with a functional allele of the Lr10 gene (such as 213, 214, 217, 220, 224, 227 et al.) identified as a result of studies are proposed to be used in breeding programs for pyramiding with other genes for resistance to leaf rust.