Improving the sperm of interspecific hybrids of the genus Ovis using biotechnological approaches
https://doi.org/10.32634/0869-8155-2020-343-11-33-35
Abstract
Relevance and methods. The efficiency of obtaining and reproduction of hybrid animals is closely related to their reproductive performance during interspecific hybridization. Hybrid males often have low fertility. In this regard, it is necessary to carry out the procedure for the preparation of semen for further use. The aim of the research was to study the effectiveness of using the flotation method (swim-up) to improve the quality of the sperm from hybrid males. The object of the study was the sperm from hybrids of argali with Romanov breed ewes (1/8 argali x 7/8 Romanov breed, n = 15).
Results. A comparative analysis of the qualitative and quantitative indicators of freshly obtained, frozen-thawed and processed by the swim-up method was carried out. The sperm freeze-thaw cycle was accompanied by an increase in the sperm DNA fragmentation index and an increase in the proportion of immotile spermatozoa by 10% and 37%, respectively, compared with freshly obtained semen. The number of sperm with abnormal morphology doubled. Using the procedure «swim-up» possible to increase the content of the progressive motile sperm by 2.1 times compared with that established for the frozen-thawed sperm (p ≤ 0,01). The obtained results indicated the effectiveness of using the “swim-up” procedure to improve the quality of the semen from interspecific hybrid males.
About the Authors
Baylar S. Iolchiev
Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst
Russian Federation
Russian Federation
Baylar S. Iolchiev, Leading Researcher, Doctor of Biological Sciences
Dubrovitsy 60, Podolsk Municipal District, Moscow Region, 142132
Ludmila A. Volkova
Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst
Russian Federation
Russian Federation
Lyudmila A. Volkova, senior researcher, candidate of biological sciences
Dubrovitsy 60, Podolsk Municipal District, Moscow Region, 142132
Natalia A. Volkova
Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst
Russian Federation
Russian Federation
Natalya A. Volkova, head of laboratory, doctor of biological sciences
Dubrovitsy 60, Podolsk Municipal District, Moscow Region, 142132
References
1. Luo J., Wang W., Sun S. Research advances in reproduction for dairy goats. Asian-Australasian Journal of Animal Sciences (AJAS). 2019;32(8):1284–1295.
2. Aibazov A.M.M., Aksyonova P.V., Seitov M.S. Modern biotechnical methods of directed reproduction of small ruminants. Bulletin of the Orenburg State Agrarian University. 2013;4(42):241–242. (In Russ.)
3. Aibazov A.M.M., Aksenova P.V., Kovalenko D.V. Biotechnological methods and techniques for intensifying the reproduction of sheep and goats. Sheep, goats, woolen business. 2013;(2):35. (In Russ.)
4. Guminskaya E.Yu., Babaeva S.S. Methods for assessing the biological usefulness of spermatozoa of bull-producers. Vestnik MDPU name I. P. Shamyakin. 2010;2(27):20–24. (In Russ.)
5. Boyko E.V., Koropets L.A., Kuzebny S.V. The relationship between the quantitative, qualitative and physiological parameters of the semen of bulls-producers of the Holstein breed. Zootechnical science of Belarus. 2015;50 (1):10–15. (In Russ.)
6. Novikov M.V., Shumilina N.N. Reproductive abilities and ways to increase the fertility of chinchilla (Chinchilla laniger Molina). Vavilov Journal of Genetics and Breeding. 2015;9(3):292–295. (In Russ.)
7. Tadzhieva A. V., Sulima N. N. Using the CASA method in assessing the quality of semen in bulls. RUDN Bulletin. Series: Agronomy and Livestock. 2015:(4):89–93. (In Russ.)
8. David I., KohnkeP., LagriffoulG. Mass sperm motility is associated with fertility in sheep. Animal Reproduction Science. 2015;(161):75–81.
9. Pourlis, A.F. A review of morphological characteristics relating to the production and reproduction of fat-tailed sheep breeds. Trop Anim Health Prod. 2011;(43):1267–1287.
10. Mohammadzadeh S., Hoseini S.A., Kadivar A. Сравнительное изучение семени баранов романовской породы и породы Lori Bakhtiari. Сельскохозяйственная биология. 2018;(2):318–325.
11. Benia A.R., Saadi M.A., Ait-Amrane A., Belhamiti T.B., Selles S.M.A.,Kaidi R. Effect of season and age on main characteristics of sperm production in the Ouled-Djellal rams. Livestock Research for Rural Development. 2018;(30):67–79.
12. Volpes A., Sammartano F., Rizzari S., Gullo, Marino A., Allegra A. The pellet swim-up is the best technique for sperm pre Separation during in vitro fertilization procedures. J Assist Reprod Genet. 2016;33(6):765–777.
Review
For citations:
Iolchiev B.S., Volkova L.A., Volkova N.A. Improving the sperm of interspecific hybrids of the genus Ovis using biotechnological approaches. Agrarian science. 2020;(11-12):33-35. (In Russ.) https://doi.org/10.32634/0869-8155-2020-343-11-33-35
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