Regeneration of plant winter garlic in vitro after colchicine treatment

Abstract

Relevance. The genetic diversity of winter garlic depends entirely on the natural selection of the best and highest-yielding clones. In unfavorable abiotic factors, physiological and biochemical properties allow polyploidy plants to have greater tolerance and viability than diploid species. Obtaining the source material based on polyploidy in winter garlic (Allium sativum L.) is one of the important alternative approaches to genetic improvement of this crop.

Methods. From air garlic bulbs, plants were grown through in vitro culture. Pre-air bulbs were treated with 0.1% colchicine solution for 1 hour, 2 hours, 4 hours. Shoots and roots were regenerated by cultivating explants for 1/2 MS, with 3 mg / l 6-BAP and 0.1 mg/l indole-3-acetic acid and cultured in light with an intensity of 5–8 ths. LC and a photoperiod of 18 hours. Treatment of air bulbs with 0.1% colchicine solution made it possible to obtain winter garlic plants using in vitro culture.

Results. In the end, we were not able to get flowering forms from garlic using the polyploidization method. But, thanks to the cultivation of winter garlic in vitro, it became possible to accelerate the selection process by obtaining one generation per year “from clove to clove”. When working on obtaining polyploidy forms, more than one variety should be used, since the reaction of varieties to colchicine treatment is ambiguous, as is their reaction to growing conditions and survival. The most responsive for obtaining plants in vitro culture was the Yubileyny Gribovsky variety.