Technology of receiving the clone rootstock gf 677 and garnem applied to the guba-khachmaz region у

Abstract

The article reflects the results of experiments on growing rootstones of stone fruits (peach, nectarine, almonds) GF 677 and Garnem in the Guba-Khachmaz region of the Republic of Azerbaijan. Important aspects of all stages of technology are highlighted: selection of the original plant material for micropropagation in vitro; micropropagation proper; growing plants in the open field. The works were carried out according to the micropropagation technique of the Institute of Plant Physiology and Genetics of the Academy of Sciences of Ukraine (1992) with innovations concerning the sterilization of explants and hormonal effects. Thus, an ideal sterilization scheme for the initial material was developed, which used a 15% solution of sodium hypochlorite as a sterilizing substance. Explants were cultured on MS medium supplemented with 1 mg/l BAP and 0.02 mg/l NAA. And for the stage of rhizogenesis, a nutrient medium was used with a concentration of 1/2 of macro- and microsalt concentration in MS, with the addition of 0.5 mg/l IBA. The resulting regenerating plants were planted in the first field of the nursery, which was laid using the technology of two-line growth of rootstocks on a black opaque mulching film. At the same time, the width of the ridges and the distance between the ridges were 50 sm. Also, a suitable irrigation system was provided for the good development of rootstocks in vitro - drip irrigation. The percentage of adapted rootstocks three months after disembarkation was 95%. The use of the technological recommendations developed by us will increase the coefficient and speed of reproduction of rootstocks, improve their quality, which will positively affect the gardening of the region and the republic as a whole.