Monitoring of indicators of chromatin status in quails ovarian follicles granulosa cells of different directions of productivity

Relevance. Identification and analysis of the reasons for reducing egg productivity is an urgent problem in quail breeding. The aim of this study is to assess the destructive processes in the somatic cells in ovarian follicles (the level of apoptosis and pycnosis) of mature quail layers of different productivity in connection with egg production and quality indicators of eggs.
Methods. In experiments, the level of apoptosis and pyknosis in granulosa cells of ovarian follicles of 3–8 mm females of Estonian, Manchurian, English white, tuxedo, English black and Pharaoh breeds was evaluated. The quails were kept in individual cages in monopreed pairs with a muted 14-hour light day. The age of the females was 160–180 days. The level of pycnotic cells was assessed by the Tarkowski cytological method, apoptotic processes were analyzed by staining with fluorochromes, propidium iodide and acridine orange (10 mcg/mlPI/AO).
Results. chromatin. The results of the study of chromatin destruction using PI/AO showed a high average percentage of apoptotic cells in almost all breeds except thep < 0.05). The maximum proportion of dead granulosa cells was tested in Tuxedo and English Black layers (52% and 43% respectively). At the same time, the English white breed of quails at the age of 183 days has one of the best indicators of egg production and the proportion of yolk in relation to egg weight, which indicates the possibility of prolonging the period of their use as laying hens.

1. Dulina A.S. The quailing as a perspective poultry industry in the Astrakhan region. Socio-economic and environmental aspects of the development of the Caspian region: Proceedings of the International Scientific and Practical Conference, Elista, May 28–30, 2019. Elista: Kalmyk State University named after B.B. Gorodovikov. 2019; 138–141. eLIBRARY ID: 41153726 (In Russian).

2. Zykov S.A. Modern trends in poultry development. Efficient animal husbandry (Effektivnoye zhivotnovodstvo). 2019; 4(152): 51–54. eLIBRARY ID: 39323601 (In Russian).

3. Herzen M.A., Korsheva I.A. Efficiency of growing quails for meat. Achievements of science and education (Dostizheniya nauki i obrazovaniya). 2018; 8(30): 48–49. (In Russian).

4. Rekhletskaya E.K., Dymkov A.B. Eggs morphological composition of japanese, pharaoh and texas white quails. Izvestia Gorsky State Agrarian University. 2019; 56(2): 66–71. eLIBRARY ID: 38470237 (In Russian).

5. Wu Y. et al. EGFR promotes the proliferation of quail follicular granulosa cells through the MAPK/extracellular signal-regulated kinase (ERK) signaling pathway. Cell Cycle. 2019; 18(20): 2742–2756. DOI: 10.1080/15384101.2019.1656952

6. German N.Yu., Vetokh A.N., Volkova N.A. Morphometric indicators of eggs from quails with different productivity types. Improving the competitiveness of animal husbandry and the tasks of staffing: materials of the XXVI International Scientific and Practical Conference. 2020; 441–445. eLIBRARY ID: 44001269 (In Russian).

7. Tarkowski A. An air-drying method for chromosomal preparation from mouse eggs. Cytogenetic and Genome Research. 1966; 1: 394–400. DOI:10.1159/000129914

8. Johnson A.L. Granulosa cell apoptosis: conservation of cell signaling in an avian ovarian model system. Biol. Signals Recept. 2000; 9(2): 96–101. DOI: 10.1159/000014628

9. Hocking P.M., Gilbert A.B., Walker M., Waddington D. Ovarian follicular structure of White Leghorns fed ad libitum and dwarf and normal broiler breeders fed ad libitum or restricted until point of lay. British poultry science. 1987; 28(3): 493–506. DOI: 10.1080/00071668708416983

10. Konstantinova I.S., Bulatova E.N., Usenko V.I. Fundamentals of cytology, general histology and embryology of animals: study guide. St. Petersburg: Publishing house «Lan». 2020; 240. eLIBRARY ID: 50018783 (In Russian).

11. Kitamura A., Yoshimura Y., Okamoto T. Changes in the populations of mitotic and apoptotic cells in white follicles during atresia in hens. Poultry science. 2022; 81(3): 408–413. DOI: 10.1093/ps/81.3.408

12. Yoshimura Y., Okamoto T., Tamura T. Ultrastructural changes of oocyte and follicular wall during oocyte maturation in the Japanese quail (Coturnix coturnix japonica). Journal of Reproduction and Fertility. 1993; 97(1): 189–196. DOI: https://doi.org/10.1530/jrf.0.0970189