Selection of Salmonella phage strains for the creation of a therapeutic and prophylactic drug against salmonellosis in piglets
https://doi.org/10.32634/0869-8155-2023-369-4-51-56
Abstract
Relevance. Bacteriophage preparations are a promising tool for the prevention and treatment of zoonoses. The effectiveness of the use of phage preparations at different stages of food production has been proven: in the cultivation of animals, in the processing of their slaughter products and in the sanitation of finished products from the causative agent of salmonellosis. The creation of an effective agent capable of protecting piglets from salmonellosis during a period of high risk of infection during weaning is an urgent task.
Methods. To study the properties of bacteriophages, methods were used to determine: lytic activity (according to Appelman and Grazia), the spectrum of their lytic action, the range of specificity, the frequency of formation of phage-resistant forms, bacterial strains selected for their reproduction. The selection of phage strains that are promising for the manufacture of a preparation based on them for the treatment and prevention of pig salmonellosis was carried out according to the criteria recommended for the selection of industrial strains of bacteriophages.
Results. A collection of 24 virulent Salmonella phages was formed. The study of their biological properties made it possible to select 4 strains of bacteriophages promising for the manufacture on their basis of a drug against swine salmonellosis.
About the Authors
S. V. Lenev
All-Russian State Center for Quality and Standardization of Medicines for Animals and Feed
Russian Federation
Russian Federation
Sergey Vasilevich Lenev, Candidate of Biology Sciences, Leading Researcher
5 Zvenigorodskoe highway, Moscow, 123022
M. K. Pirozhkov
All-Russian State Center for Quality and Standardization of Medicines for Animals and Feed
Russian Federation
Russian Federation
Mikhail Konstantinovich Pirozhkov, Doctor of Veterinary Sciences, Leading Researcher
5 Zvenigorodskoe highway, Moscow, 123022
A. V. Motorygin
All-Russian State Center for Quality and Standardization of Medicines for Animals and Feed
Russian Federation
Russian Federation
Anton Valerievich Motorygin, Candidate of Veterinary Sciences, chef laboratory
5 Zvenigorodskoe highway, Moscow, 123022
A. A. Galiakbarova
All-Russian State Center for Quality and Standardization of Medicines for Animals and Feed
Russian Federation
Russian Federation
Alsu Anvarovna Galiakbarova, Researcher
5 Zvenigorodskoe highway, Moscow, 123022
A. S. Klenov
All-Russian State Center for Quality and Standardization of Medicines for Animals and Feed
Russian Federation
Russian Federation
Alexey Sergeevich Klenov, Junior Researcher
5 Zvenigorodskoe highway, Moscow, 123022
N. S. Abrosimova
All-Russian State Center for Quality and Standardization of Medicines for Animals and Feed
Russian Federation
Russian Federation
Nadezhda Sergeevna Abrosimova, Researcher
5 Zvenigorodskoe highway, Moscow, 123022
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Review
For citations:
Lenev S.V., Pirozhkov M.K., Motorygin A.V., Galiakbarova A.A., Klenov A.S., Abrosimova N.S. Selection of Salmonella phage strains for the creation of a therapeutic and prophylactic drug against salmonellosis in piglets. Agrarian science. 2023;(4):51-56. (In Russ.) https://doi.org/10.32634/0869-8155-2023-369-4-51-56
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