Optimization of submersible cultivation of mycelium Pleurotus ostreatus in the dynamics of growth in terms of lipid peroxidation

Relevance. The work is related to the improvement of artificial cultivation processes of affordable and convenient (from a biotechnological point of view) producers of Pleurotus ostreatus food biomass. Some stages of mycelium cultivation technology have been optimized, in particular, changes in the conditions of oyster mushroom mycelium cultivation through the use of magnesium carbonate as an additive to the nutrient medium when obtaining seed material for growing mushroom fruit bodies have been proposed. The introduction of the proposed cultivation conditions into the practice of mushroom farming will potentially allow for maximum biomass yield during submerged cultivation of basidiomycetes in liquid nutrient media. 

Methods. The cultivation of mycelium was carried out by the method of submersible cultivation. The protein concentration in the solution was determined by the Bradford method, lipid peroxidation (POL) — by the content of TBK-active products (2-thiobarbituric acid, TBK), the total content of which was expressed in terms of malonic dialdehyde (MDA). The data obtained were processed by multivariate regression using the Principal component method (PCA). 

Results. It has been shown that low concentrations of magnesium carbonate have a positive effect on the growth of mycelium biomass. For the first time, the obtained data on the intensity of lipid peroxidation (POL) and protein concentration in solution were processed by mathematical regression. It was found that the use of magnesium carbonate at a concentration of 1x10^-6 positively affects the growth of mycelium biomass of the fungus Pleurotus ostreatus with an increase in mycelium biomass by 20.5% compared with the control. 

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