Application of near infrared spectroscopy for identification and quantitative determination of amino acids in their crystalline and salt forms in the preparation of animal feed

Methods. For the first time, a comprehensive calibration model has been developed and presented for the rapid determination of basic near-infrared spectroscopy (IR) methods in feed amino acids, with application in the production of animal feed. The research principle is based on the Fourier equation for spectroscopy. In this work, Fourier methods in the Italian region (FTIR, FT-NIRS) were applied. The data obtained from the calibration models were confirmed using high-performance liquid chromatography. FT-NIR predictions agreed well with the chromatography data and had predictive deviation (RPD) values >1.3 in all cases.
Results. The results indicate that FT-NIR spectroscopy can be used as a simple and rapid tool for monitoring amino acids. In the course of the work, experimental confirmation of previously known facts was obtained — the possibility of visual separation of the spectra of not only counterfeit amino acids, but also the possibility of separation by L- and DL-optical isomers. The work shows that the discrepancies between the values obtained by the classical “wet chemistry” method and the values obtained from the constructed calibration models do not exceed the reproducibility limits of arbitration methods. A predictive model was used based on information flow elements using the OPUS/QUANT2 software package for multivariate calibration and construction of calibration models for amino acids. This chemometric analysis proved the fundamental possibility of determining amino acids in the product. It has been shown that the use of information channels opens up opportunities for the use of many chemometrics algorithms, including data preprocessing and the construction of predictive models.

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