A new antimicrobial food peptide: characteristics, properties and effectiveness evaluation
https://doi.org/10.32634/0869-8155-2024-381-4-132-137
Abstract
Promising candidates as antibacterial drugs are antimicrobial peptides (AMP) present in cow colostrum. The aim of the research is to isolate, theoretically substantiate and confirm the biological activity of a new AMP from pepsin hydrolysate of cow colostrum with the amino acid sequence EKLAKNKLARGLKRK. According to the Protein NCBI database, the isolated AMP is identified as AW655195.1 105840 MARC 1BOV/ Bos taurus (cattle). When identifying AMP from the database of antimicrobial peptides APD, it was not found. When identifying AMP using the DRAMP database, the object under study is missing, but its characteristics belong to the antimicrobial class. The total hydrophobic ratio of the peptide determined by APD is 33%. The total net charge is +6, the molecular weight is 1753.151 Da. Protein binding potential (Boman index) at 3.44 kcal/mol. The selected AMP is close in characteristics to the known ones included in the APD database. AMP belongs to the cationic hydrophobic α-helical, which suggests its antimicrobial activity. As a result of in vitro experiments, the antimicrobial and antitumor activity of the peptide has been proven.
About the Authors
E. A. Ulitina
Ural State Agrarian University
Russian Federation
Russian Federation
Elizaveta Andreevna Ulitina, Postgraduate Student
Sh. S. Valieva
Ural State Agrarian University
Russian Federation
Russian Federation
Sholpan Sergeevna Valieva, Postgraduate Student
42 Karl Liebknecht Str., Yekaterinburg, 620075
S. L. Tikhonov
Ural State Agrarian University; Ural State Forestry Engineering University
Russian Federation
Russian Federation
Sergey Leonidovich Tikhonov, Doctor of Technical Sciences, Professor
42 Karl Liebknecht Str., Yekaterinburg, 620075
37 Siberian Tract, Yekaterinburg, 620100
N. V. Tikhonova
Ural State Agrarian University
Russian Federation
Russian Federation
Natalia Valeryevna Tikhonova, Doctor of Technical Sciences, Professor
42 Karl Liebknecht Str., Yekaterinburg, 620075
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Review
For citations:
Ulitina E.A., Valieva Sh.S., Tikhonov S.L., Tikhonova N.V. A new antimicrobial food peptide: characteristics, properties and effectiveness evaluation. Agrarian science. 2024;(4):132-137. (In Russ.) https://doi.org/10.32634/0869-8155-2024-381-4-132-137
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